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Abstract Interstitial flow is an important component of the microcirculation and interstitial environment, yet its effects on cell organization and tissue architecture are poorly understood, in part due to the lack of in vitro models. To examine the effects of interstitial flow on cell morphology and matrix remodeling, we developed a tissue culture model that physically supports soft tissue cultures and allows microscopic visualization of cells within the three-dimensional matrix. In addition, pressure-flow relationships can be continuously monitored to evaluate the bulk hydraulic resistance as an indicator of changes in the overall matrix integrity. We observed that cells such as human dermal fibroblasts aligned perpendicular to the direction of interstitial flow. In contrast, fibroblasts in static three-dimensional controls remained randomly oriented, whereas cells subjected to fluid shear as a two-dimensional monolayer regressed. Also, the dynamic measurements of hydraulic conductivity suggest reorganization toward a steady state. These primary findings help establish the importance of interstitial flow on the biology of tissue organization and interstitial fluid balance. cell culture shear stress mechanical stress hydraulic conductivity Footnotes This work was supported by the Whitaker Foundation and the National Science Foundation. Address for reprint requests and other correspondence: M. A. Swartz, Dept. of Biomedical Engineering, Northwestern Univ., 2145 Sheridan Rd., Evanston, IL 60208-3107 (E-mail: m-swartz2@northwestern.edu ). The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “ advertisement ” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. First published January 16, 2003;10.1152/ajpheart.01008.2002 Copyright © 2003 the American Physiological Society
AJP - Heart and Circulatory Physiology – The American Physiological Society
Published: May 1, 2003
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