Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Molecular detection and genotyping of human noroviruses in influent and effluent water at a wastewater treatment plant in Japan

Molecular detection and genotyping of human noroviruses in influent and effluent water at a... Aims: To investigate the prevalence, seasonality and genotype distribution of human noroviruses (NoVs) in wastewater in Japan. Methods and Results: Influent and effluent water samples were collected monthly for a year from a wastewater treatment plant and examined for the presence of genogroups I and II (GI and GII) NoVs. Using real‐time reverse transcription (RT)‐PCR assays, 12 (100%) influent and six (50%) effluent samples were positive for both GI and GII NoV genomes, with a higher prevalence in winter. A total of 152 different NoV strains, comprising 84 GI and 68 GII strains, were identified using seminested RT‐PCR assays followed by cloning and sequence analysis. These strains were classified into nine GI genotypes (GI/1, 2, 4, 5, 8, 9, 11, 12 and 14) and 13 GII genotypes (GII/1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 15 and 16), showing considerable genetic diversity. Conclusions: Based on the partial capsid gene sequences, we identified a great number of NoV strains belonging to many genotypes, demonstrating that genetically diverse NoV strains are co‐circulating in aquatic environments and human populations. Significance and Impact of the Study: Our results clearly demonstrate the seasonal trend and genetic diversity of NoVs in wastewater. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Microbiology Wiley

Molecular detection and genotyping of human noroviruses in influent and effluent water at a wastewater treatment plant in Japan

Loading next page...
 
/lp/wiley/molecular-detection-and-genotyping-of-human-noroviruses-in-influent-dwy4yr8ecp

References (30)

Publisher
Wiley
Copyright
© 2012 The Authors. Journal of Applied Microbiology © 2012 The Society for Applied Microbiology
ISSN
1364-5072
eISSN
1365-2672
DOI
10.1111/j.1365-2672.2012.05231.x
pmid
22221550
Publisher site
See Article on Publisher Site

Abstract

Aims: To investigate the prevalence, seasonality and genotype distribution of human noroviruses (NoVs) in wastewater in Japan. Methods and Results: Influent and effluent water samples were collected monthly for a year from a wastewater treatment plant and examined for the presence of genogroups I and II (GI and GII) NoVs. Using real‐time reverse transcription (RT)‐PCR assays, 12 (100%) influent and six (50%) effluent samples were positive for both GI and GII NoV genomes, with a higher prevalence in winter. A total of 152 different NoV strains, comprising 84 GI and 68 GII strains, were identified using seminested RT‐PCR assays followed by cloning and sequence analysis. These strains were classified into nine GI genotypes (GI/1, 2, 4, 5, 8, 9, 11, 12 and 14) and 13 GII genotypes (GII/1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 15 and 16), showing considerable genetic diversity. Conclusions: Based on the partial capsid gene sequences, we identified a great number of NoV strains belonging to many genotypes, demonstrating that genetically diverse NoV strains are co‐circulating in aquatic environments and human populations. Significance and Impact of the Study: Our results clearly demonstrate the seasonal trend and genetic diversity of NoVs in wastewater.

Journal

Journal of Applied MicrobiologyWiley

Published: Mar 1, 2012

There are no references for this article.