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antly fasttwitch fibers (28). The s were cubated a shakg cubator at 35°C 2 ml Krebs-Henseleit bicarbonate buffer (KHB) (24) that contaed 0.1% bove serum album, 32 mM mannitol, 8 mM , and various additions stoppered Erlenmeyer flasks with a gas phase either 95% O,-5% CO, or 95% N,-5% CO, (). To keep osmolarity constant throughout an experent, sufficient mannitol was cluded the medium to keep the sum the concentrations or 3-MG plus mannitol at 40 mM. Specific formation regardg the additions to the cubation medium is provided for each experent. contraction. The âs distal tendon was attached to a vertical Lucite rod contag two platum electrodes (12). The proxal tendon was clipped to a jewellerâs cha that connected it to a force transducer (Grass model FTOC), and restg tension was adjusted to 0.5 g. The mounted was mersed 20 ml KHB mataed at 35°C and contuously oxygenated with 95% O,-5% CO,. The s were stulated wit.h supramaxal square-wave pulses 0.2-ms duration with a Grass S48 stulator. The tetanic contractions were elicited stulatg at 100 Hz for 10 s at a rate 1 contraction/m for 10 m. When s were stulated after cubation hypoxic medium, they were allowed
Journal of Applied Physiology – The American Physiological Society
Published: Apr 1, 1991
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