Access the full text.
Sign up today, get DeepDyve free for 14 days.
R. Heim, D.C. Prasher, R.Y. Tsien (1994)
Wavelength mutations and posttranslational autoxidation of green fluorescent protein. ProceedingsNatl Acad. Sci. USA, 91
I. Hohl, D. Robinson, M. Chrispeels, G. Hinz (1996)
Transport of storage proteins to the vacuole is mediated by vesicles without a clathrin coat.Journal of cell science, 109 ( Pt 10)
G. Hewitt (1996)
Some genetic consequences of ice ages, and their role in divergence and speciationBiological Journal of The Linnean Society, 58
L. Gómez, M. Chrispeels (1993)
Tonoplast and Soluble Vacuolar Proteins Are Targeted by Different Mechanisms.The Plant cell, 5
Kenji Matsuoka, T. Higuchi, M. Maeshima, K. Nakamura (1997)
A Vacuolar-Type H+-ATPase in a Nonvacuolar Organelle Is Required for the Sorting of Soluble Vacuolar Protein Precursors in Tobacco Cells.The Plant cell, 9
M. Ehara, T. Noguchi, K. Ueda (1996)
Uptake of Neutral Red by the Vacuoles of a Green Alga, Micrasterias pinnatifidaPlant and Cell Physiology, 37
J.I. Nagy, P. Maliga (1976)
Callus induction and plant regeneration from mesophyll protoplasts ofNicotiana Sylvestris. Z. Pflanzenphysiol., 78
James Dombrowski, Martin Schroeder, Sebastian Bednarek, Natasha Raikhel (1993)
Determination of the functional elements within the vacuolar targeting signal of barley lectin.The Plant cell, 5
R. Heim, D. Prasher, R. Tsien (1994)
Wavelength mutations and posttranslational autoxidation of green fluorescent protein.Proceedings of the National Academy of Sciences of the United States of America, 91 26
J.‐M. Neuhaus, J.C. Rogers (1998)
Sorting of proteins to vacuoles in plant cells.Plant Mol. Biol.
J. Neuhaus, L. Sticher, Frederick Meins, Thomas Boller (1991)
A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole.Proceedings of the National Academy of Sciences of the United States of America, 88 22
E. Freydl, F. Meins, T. Boller, J.‐M. Neuhaus (1995)
Kinetics of prolyl hydroxylation, intracellular transport and C‐terminal processing of the tobacco vacuolar chitinase.Planta, 147
I. Negrutiu, R.D. Shillito, I. Potrykus, G. Biasini, F. Sala (1987)
Hybrid genes in the analysis of transformation conditions. I. Setting up a simple method for direct gene transfer in plant protoplasts.Plant Mol. Biol., 8
J. Neuhaus, M. Pietrzak, T. Boller (1994)
Mutation analysis of the C-terminal vacuolar targeting peptide of tobacco chitinase: low specificity of the sorting system, and gradual transition between intracellular retention and secretion into the extracellular space.The Plant journal : for cell and molecular biology, 5 1
Ken Matsuoka, Diane Bassham, N. Raikhel, Kenzo Nakamura (1995)
Different sensitivity to wortmannin of two vacuolar sorting signals indicates the presence of distinct sorting machineries in tobacco cellsThe Journal of Cell Biology, 130
S. Grabski, Adriaan de, Feijter, Melvin Schindler (1993)
Endoplasmic Reticulum Forms a Dynamic Continuum for Lipid Diffusion between Contiguous Soybean Root Cells.The Plant cell, 5
N. Paris, C. Stanley, Russell Jones, J. Rogers (1996)
Plant Cells Contain Two Functionally Distinct Vacuolar CompartmentsCell, 85
J. Haseloff, K. Siemering, D. Prasher, Sarah Hodge (1997)
Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly.Proceedings of the National Academy of Sciences of the United States of America, 94 6
J.‐M. Neuhaus, L. Sticher, F. Meins, T. Boller (1991)
A short C‐terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole. ProceedingsNatl Acad. Sci. USA, 88
B. Holwerda, H. Padgett, John Rogers (1992)
Proaleurain vacuolar targeting is mediated by short contiguous peptide interactions.The Plant cell, 4
J. Sambrook, E.F. Fritsch, T. Maniatis (1989)
Molecular Cloning: a Laboratory ManualPlant Physiol.
M. Schroeder, O. Borkhsenious, K. Matsuoka, K. Nakamura, N. Raikhel (1993)
Colocalization of Barley Lectin and Sporamin in Vacuoles of Transgenic Tobacco Plants, 101
T. Okita, J. Rogers (1996)
COMPARTMENTATION OF PROTEINS IN THE ENDOMEMBRANE SYSTEM OF PLANT CELLS.Annual review of plant physiology and plant molecular biology, 47
K. Nakamura, K. Matsuoka, F. Mukumoto, N. Watanabe (1993)
Processing and transport to the vacuole of a precursor to sweet potato sporamin in transformed tobacco cell line BY-2Journal of Experimental Botany, 44
P. Boevink, S. Cruz, C. Hawes, N. Harris, K. Oparka (1996)
Virus‐mediated delivery of the green fluorescent protein to the endoplasmic reticulum of plant cellsPlant Journal, 10
J. Haseloff, R.K. Siemering, D.C. Prasher, S. Hodge (1997)
Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly. ProceedingsNatl Acad. Sci. USA, 94
J. Nagy, P. Maliga (1976)
Callus Induction and Plant Regeneration from Mesophyll Protoplasts of Nicotiana sylvestrisZeitschrift für Pflanzenphysiologie, 78
K. Siemering, R. Golbik, R. Sever, J. Haseloff (1996)
Mutations that suppress the thermosensitivity of green fluorescent proteinCurrent Biology, 6
The green fluorescent protein (GFP) from Aequorea victoria can be detected in living plant cells after transient transformation of protoplasts. Expression of the GFP can be used to monitor protein trafficking in a mixed cell population and also to study the different function and importance of organelles in different cell types. We developed a vacuolar form of GFP that was obtained by replacing the C‐terminal endoplasmic reticulum (ER)‐retention motif of mGFP5‐ER by the vacuolar targeting peptide of tobacco chitinase A. The vacuolar GFP was transported and accumulated in the vacuole as expected. However, we found two patterns of GFP accumulation after prolonged incubation (18–24 h) depending on the cell type. Most chloroplast‐rich protoplasts had a fluorescent large central vacuole. In contrast, most chloroplast‐poor protoplasts accumulated the GFP in one smaller vacuole but not in the large central vacuole, which was visible under a light microscope in the same cell. This differential accumulation reflected the existence of two different vacuolar compartments as described recently by immunolocalization of several vacuolar markers. We were able to characterize the vacuolar compartment to which GFP is specifically targeted as non‐acidic, since it did not accumulate neutral red while acidic vacuoles did not accumulate GFP.
The Plant Journal – Wiley
Published: Aug 1, 1998
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.