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Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen Acceptor

Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen Acceptor Ann. din. Biochem. 6 (1969) 24 P. TRINDER Biochemistry Department, CentralLaboratory, Royal Infirmary, Sunderland In the reactions the volume to 11. with water. Keeps indefinitely. Colour reagent. 7S mI. 4% NlltiHPO.; 215 mI. Glucose + glucose oxidase + O, ..... Gluconic Acid water, 5 mI. Fermcozyme 653 AM glucose oxidase +H.O. and Hughes Ltd., Brentwood, Essex: (Hughes HzO + Peroxidase + Oxygen acceptor (Colourless) Fermco Laboratories, PO Box 5110, Chicago, Ill.) ..... H.O + Oxidised acceptor (Coloured) 5 ml. 0.1 % peroxidase R.Z. 0.6; add 300 mg. and 100 mg. 4-amino phenazone. sodium azide the oxygen acceptors originally used were 0 tolidine, Keeps at least 8 weeks at 4°C. and o-dianisidine. It has since been benzidine Procedure Add 0.1 ml. blood to 2.9 ml. protein established that these three substances are carcino­ precipitant. Centrifuge and transfer 1 ml, clear gens and many alternative oxygen acceptors have supernatant to a t in. tube labelled U. Place 1 ml. Any dye showing oxidation-reduc­ been suggested. protein precipitant in a tube labelled B. Place 1 ml. tion properties or any dye formed by oxidation, standard, comprising a mixture of 0.1 rnl, glucose such as those used in colour photography, are solution (200 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annals of Clinical Biochemistry: An International Journal of Biochemistry and Laboratory Medicine SAGE

Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen Acceptor

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Publisher
SAGE
Copyright
© 1969 Association for Clinical Biochemistry
ISSN
0004-5632
eISSN
1758-1001
DOI
10.1177/000456326900600108
Publisher site
See Article on Publisher Site

Abstract

Ann. din. Biochem. 6 (1969) 24 P. TRINDER Biochemistry Department, CentralLaboratory, Royal Infirmary, Sunderland In the reactions the volume to 11. with water. Keeps indefinitely. Colour reagent. 7S mI. 4% NlltiHPO.; 215 mI. Glucose + glucose oxidase + O, ..... Gluconic Acid water, 5 mI. Fermcozyme 653 AM glucose oxidase +H.O. and Hughes Ltd., Brentwood, Essex: (Hughes HzO + Peroxidase + Oxygen acceptor (Colourless) Fermco Laboratories, PO Box 5110, Chicago, Ill.) ..... H.O + Oxidised acceptor (Coloured) 5 ml. 0.1 % peroxidase R.Z. 0.6; add 300 mg. and 100 mg. 4-amino phenazone. sodium azide the oxygen acceptors originally used were 0 tolidine, Keeps at least 8 weeks at 4°C. and o-dianisidine. It has since been benzidine Procedure Add 0.1 ml. blood to 2.9 ml. protein established that these three substances are carcino­ precipitant. Centrifuge and transfer 1 ml, clear gens and many alternative oxygen acceptors have supernatant to a t in. tube labelled U. Place 1 ml. Any dye showing oxidation-reduc­ been suggested. protein precipitant in a tube labelled B. Place 1 ml. tion properties or any dye formed by oxidation, standard, comprising a mixture of 0.1 rnl, glucose such as those used in colour photography, are solution (200

Journal

Annals of Clinical Biochemistry: An International Journal of Biochemistry and Laboratory Medicine – SAGE

Published: Jan 1, 1969

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