Access the full text.
Sign up today, get DeepDyve free for 14 days.
H. Kuang, S. Woo, B. Meyers, E. Nevo, R. Michelmore (2004)
Multiple Genetic Processes Result in Heterogeneous Rates of Evolution within the Major Cluster Disease Resistance Genes in Lettucew⃞The Plant Cell Online, 16
EB Mains, CE Leighty, CO Johnston (1926)
Inheritance of resistance to leaf rust Puccinia triticina Erikss., in crosses of common wheat, Triticum vulgare VillJ Agric Res, 32
E. Bossolini, T. Wicker, P. Knobel, B. Keller (2007)
Comparison of orthologous loci from small grass genomes Brachypodium and rice: implications for wheat genomics and grass genome annotation.The Plant journal : for cell and molecular biology, 49 4
J. Kolmer (2005)
Tracking wheat rust on a continental scale.Current opinion in plant biology, 8 4
M. Jordan (2000)
Green fluorescent protein as a visual marker for wheat transformationPlant Cell Reports, 19
S. Nilmalgoda, S. Cloutier, A. Walichnowski (2003)
Construction and characterization of a bacterial artificial chromosome (BAC) library of hexaploid wheat (Triticum aestivum L.) and validation of genome coverage using locus-specific primers.Genome, 46 5
Liuling Yan, A. Loukoianov, G. Tranquilli, M. Helguera, T. Fahima, J. Dubcovsky (2003)
Positional cloning of the wheat vernalization gene VRN1Proceedings of the National Academy of Sciences of the United States of America, 100
Liuling Yan, A. Loukoianov, A. Blechl, G. Tranquilli, W. Ramakrishna, P. SanMiguel, J. Bennetzen, V. Echenique, J. Dubcovsky (2004)
The Wheat VRN2 Gene Is a Flowering Repressor Down-Regulated by VernalizationScience, 303
M. Luo, Carolyn Thomas, Frank You, J. Hsiao, Ouyang Shu, C. Buell, M. Malandro, P. McGuire, Olin Anderson, J. Dvorak (2003)
High-throughput fingerprinting of bacterial artificial chromosomes using the snapshot labeling kit and sizing of restriction fragments by capillary electrophoresis.Genomics, 82 3
C. Feuillet, S. Travella, N. Stein, L. Albar, Aurélie Nublat, B. Keller (2003)
Map-based isolation of the leaf rust disease resistance gene Lr10 from the hexaploid wheat (Triticum aestivum L.) genomeProceedings of the National Academy of Sciences of the United States of America, 100
L. Iyer, S. Kumpatla, M. Chandrasekharan, T. Hall (2000)
Transgene silencing in monocotsPlant Molecular Biology, 43
J. Bai, L. Pennill, Jianchang Ning, Se Lee, J. Ramalingam, C. Webb, Bingyu Zhao, Qing Sun, J. Nelson, J. Leach, S. Hulbert (2002)
Diversity in nucleotide binding site-leucine-rich repeat genes in cereals.Genome research, 12 12
Hong-Bin Zhang, Xinping Zhao, Xiaoling Ding, A. Paterson, R. Wing (1995)
Preparation of megabase‐size DNA from plant nucleiPlant Journal, 7
J. Kolmer (2001)
Physiologic Specialization of Puccinia triticina in Canada in 1998.Plant disease, 85 2
ER Ausemus, JB Harrington, WW Worzella (1946)
A summary of genetic studies in hexaploid and tetraploid wheatsJ Am Soc Agron, 38
Steven Brooks, Li Huang, Marie Herbel, B. Gill, G. Brown-Guedira, J. Fellers (2006)
Structural variation and evolution of a defense-gene cluster in natural populations of Aegilops tauschiiTheoretical and Applied Genetics, 112
H. Ling, J. Qiu, R. Singh, B. Keller (2004)
Identification and genetic characterization of an Aegilops tauschii ortholog of the wheat leaf rust disease resistance gene Lr1Theoretical and Applied Genetics, 109
A. Lupas (1996)
Coiled coils: new structures and new functions.Trends in biochemical sciences, 21 10
P. Dyck, D. Samborski (1968)
GENETICS OF RESISTANCE TO LEAF RUST IN THE COMMON WHEAT VARIETIES WEBSTER, LOROS, BREVIT, CARINA, MALAKOF AND CENTENARIOCanadian journal of genetics and cytology, 10
G. Martin, A. Bogdanove, G. Sessa (2003)
Understanding the functions of plant disease resistance proteins.Annual review of plant biology, 54
A. Christensen, P. Quail (1996)
Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plantsTransgenic Research, 5
P. Lehmann (2002)
Structure and evolution of plant disease resistance genes.Journal of applied genetics, 43 4
B. Keller, C. Feuillet, N. Yahiaoui (2005)
Map-based isolation of disease resistance genes from bread wheat: cloning in a supersize genome.Genetical research, 85 2
Steve Holzberg, P. Brosio, C. Gross, G. Pogue (2002)
Barley stripe mosaic virus-induced gene silencing in a monocot plant.The Plant journal : for cell and molecular biology, 30 3
H. Vaucheret, C. Beclin, T. Elmayan, F. Feuerbach, C. Godon, J. Morel, P. Mourrain, J. Palauqui, S. Vernhettes (1998)
Transgene-induced gene silencing in plants.The Plant journal : for cell and molecular biology, 16 6
S. Scofield, Li Huang, Amanda Brandt, B. Gill (2005)
Development of a Virus-Induced Gene-Silencing System for Hexaploid Wheat and Its Use in Functional Analysis of the Lr21-Mediated Leaf Rust Resistance Pathway1Plant Physiology, 138
A. Lupas, M. Dyke, J. Stock (1991)
Predicting coiled coils from protein sequencesScience, 252
D. Mcvey (1989)
Verification of infection-type data for identification of genes for resistance to leaf rust in some hard red spring wheatCrop Science, 29
B. Meyers, K. Shen, P. Rohani, B. Gaut, R. Michelmore (1998)
Receptor-like Genes in the Major Resistance Locus of Lettuce Are Subject to Divergent SelectionPlant Cell, 10
Qianhua Shen, Y. Saijo, S. Mauch, C. Biskup, S. Bieri, B. Keller, H. Seki, Bekir Ülker, I. Somssich, P. Schulze-Lefert (2007)
Nuclear Activity of MLA Immune Receptors Links Isolate-Specific and Basal Disease-Resistance ResponsesScience, 315
Maike Tam, J. Oseph, N. Mol, M. Ooter, Biocentrum Amsterdam (1997)
THE SILENCE OF GENES IN TRANSGENIC PLANTSAnnals of Botany, 79
C. Feuillet, M. Messmer, G. Schachermayr, B. Keller (1995)
Genetic and physical characterization of theLR1 leaf rust resistance locus in wheat (Triticum aestivum L.)Molecular and General Genetics MGG, 248
C. Webb, J. Fellers (2006)
Cereal rust fungi genomics and the pursuit of virulence and avirulence factors.FEMS microbiology letters, 264 1
L. Madsen, N. Collins, M. Rakwalska, G. Backes, N. Sandal, Lene Krusell, Lene Krusell, J. Jensen, E. Waterman, A. Jahoor, M. Ayliffe, A. Pryor, P. Langridge, P. Schulze-Lefert, J. Stougaard (2003)
Barley disease resistance gene analogs of the NBS-LRR class: identification and mappingMolecular Genetics and Genomics, 269
AC Majestic, AC Splendor, J. Kolmer, J. Liu (2002)
Inheritance of leaf rust resistance in the wheat cultivars AC Majestic, AC Splendor, and AC KarmaCanadian Journal of Plant Pathology, 24
D. Laurie, M. Bennett (1986)
Wheat × maize hybridizationCanadian journal of genetics and cytology, 28
B. Meyers, S. Kaushik, Raja Nandety (2005)
Evolving disease resistance genes.Current opinion in plant biology, 8 2
J. Kolmer, P. Dyck (1994)
Gene expression in the Triticum aestivum-Puccinia recondita f.sp. tritici gene-for-gene systemPhytopathology, 84
H. Flor (1956)
The Complementary Genic Systems in Flax and Flax RustAdvances in Genetics, 8
Li Huang, Steven Brooks, Wanlong Li, J. Fellers, H. Trick, B. Gill (2003)
Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of bread wheat.Genetics, 164 2
Tessa Burch-Smith, Jeffrey Anderson, Jeffrey Anderson, G. Martin, G. Martin, S. Dinesh-Kumar (2004)
Applications and advantages of virus-induced gene silencing for gene function studies in plants.The Plant journal : for cell and molecular biology, 39 5
G. Schachermayr, M. Messmer, C. Feuillet, H. Winzeler, M. Winzeler, B. Keller (1995)
Identification of molecular markers linked to the Agropyron elongatum-derived leaf rust resistance gene Lr24 in wheatTheoretical and Applied Genetics, 90
A. Graner, H. Siedler, A. Jahoor, Reinhold Herrmann, Gerhard Wenzel (1990)
Assessment of the degree and the type of restriction fragment length polymorphism in barley (Hordeum vulgare)Theoretical and Applied Genetics, 80
D. Long, J. Kolmer (1989)
A North American system of nomenclature for Puccinia recondita f. sp. triticiPhytopathology, 79
T. Zhou, Y. Wang, Jian-Qun Chen, H. Araki, Zhiqiang Jing, K. Jiang, J. Shen, Dacheng Tian (2004)
Genome-wide identification of NBS genes in japonica rice reveals significant expansion of divergent non-TIR NBS-LRR genesMolecular Genetics and Genomics, 271
N. Stein, C. Feuillet, T. Wicker, E. Schlagenhauf, B. Keller (2000)
Subgenome chromosome walking in wheat: a 450-kb physical contig in Triticum monococcum L. spans the Lr10 resistance locus in hexaploid wheat (Triticum aestivum L.).Proceedings of the National Academy of Sciences of the United States of America, 97 24
R. Mcintosh, E. Baker, C. Driscoll (1965)
Cytogenetical Studies in Wheat I. Monosomic Analysis of Leaf Rust Resistance in the Cultivars Uruguay and TransferAustralian Journal of Biological Sciences, 18
F. Gallego, C. Feuillet, M. Messmer, A. Penger, A. Graner, M. Yano, Takuji Sasaki, B. Keller (1998)
Comparative mapping of the two wheat leaf rust resistance loci Lr1 and Lr10 in rice and barley.Genome, 41 3
C. Arrecubieta, E. García, R. Lopez (1995)
Sequence and transcriptional analysis of a DNA region involved in the production of capsular polysaccharide in Streptococcus pneumoniae type 3.Gene, 167 1-2
S. Hulbert, C. Webb, Shavannor Smith, Qing Sun (2001)
Resistance gene complexes: evolution and utilization.Annual review of phytopathology, 39
W. Chiu, Y. Niwa, Weike Zeng, T. Hirano, Hirokazu Kobayashi, J. Sheen (1996)
Engineered GFP as a vital reporter in plantsCurrent Biology, 6
J. Kolmer (1996)
Genetics of resistance to wheat leaf rust.Annual review of phytopathology, 34
DJ Samborski (1973)
Leaf rust of wheat in Canada in (1972)Can Plant Dis Surv, 52
D. Somers, P. Isaac, K. Edwards (2004)
A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.)Theoretical and Applied Genetics, 109
J. Thompson, D. Higgins, T. Gibson (1994)
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.Nucleic acids research, 22 22
J. Dangl, Jonathan Jones (2001)
Plant pathogens and integrated defence responses to infectionNature, 411
S. Altschul, Thomas Madden, A. Schäffer, Jinghui Zhang, Zheng Zhang, W. Miller, D. Lipman (1997)
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.Nucleic acids research, 25 17
D. Leister (2004)
Tandem and segmental gene duplication and recombination in the evolution of plant disease resistance gene.Trends in genetics : TIG, 20 3
B. Meyers, A. Kozik, A. Griego, H. Kuang, R. Michelmore (2003)
Genome-Wide Analysis of NBS-LRR–Encoding Genes in Arabidopsis Online version contains Web-only data. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.009308.The Plant Cell Online, 15
D. Xie, K. Devos, G. Moore, M. Gale (1993)
RFLP-based genetic maps of the homoeologous group 5 chromosomes of bread wheat (Triticum aestivum L.)Theoretical and Applied Genetics, 87
E. Biezen, J. Jones (1998)
Plant disease-resistance proteins and the gene-for-gene concept.Trends in biochemical sciences, 23 12
E. Lander, E. Lander, P. Green, Jeff Abrahamson, A. Barlow, M. Daly, S. Lincoln, Lee Newburg (1987)
MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations.Genomics, 1 2
PL Dyck, DJ Samborski, JW Martens (1985)
Inheritance of resistance to leaf rust and stem rust in the wheat cultivar GlenleaCan J Plant Pathol, 7
M. Graham, L. Marek, R. Shoemaker (2002)
Organization, expression and evolution of a disease resistance gene cluster in soybean.Genetics, 162 4
J. Liu, J. Kolmer (1998)
Genetics of Stem Rust Resistance in Wheat Cvs. Pasqua and AC Taber.Phytopathology, 88 2
BC Meyers, A Kozik, A Griego (2003)
Genome-wide analysis of NBS-LRR-encoding genes in ArabidopsisPlant Cell, 15
H. Ling, Yuxing Zhu, Beat Keller (2003)
High-resolution mapping of the leaf rust disease resistance gene Lr1 in wheat and characterization of BAC clones from the Lr1 locusTheoretical and Applied Genetics, 106
E. Sonnhammer, R. Durbin (1995)
A dot-matrix program with dynamic threshold control suited for genomic DNA and protein sequence analysis.Gene, 167 1-2
N. Yahiaoui, P. Srichumpa, R. Dudler, B. Keller (2004)
Genome analysis at different ploidy levels allows cloning of the powdery mildew resistance gene Pm3b from hexaploid wheat.The Plant journal : for cell and molecular biology, 37 4
M. Stam, J. Mol, J. Kooter (1997)
Review Article: The Silence of Genes in Transgenic PlantsAnnals of Botany, 79
N. Radovanović, S. Cloutier (2003)
Gene-assisted selection for high molecular weight glutenin subunits in wheat doubled haploid breeding programsMolecular Breeding, 12
In hexaploid wheat, leaf rust resistance gene Lr1 is located at the distal end of the long arm of chromosome 5D. To clone this gene, an F1-derived doubled haploid population and a recombinant inbred line population from a cross between the susceptible cultivar AC Karma and the resistant line 87E03-S2B1 were phenotyped for resistance to Puccinia triticina race 1-1 BBB that carries the avirulence gene Avr1. A high-resolution genetic map of the Lr1 locus was constructed using microsatellite, resistance gene analog (RGA), BAC end (BE), and low pass (LP) markers. A physical map of the locus was constructed by screening a hexaploid wheat BAC library from cultivar Glenlea that is known to have Lr1. The locus comprised three RGAs from a gene family related to RFLP marker Xpsr567. Markers specific to each paralog were developed. Lr1 segregated with RGA567-5 while recombinants were observed for the other two RGAs. Transformation of the susceptible cultivar Fielder with RGA567-5 demonstrated that it corresponds to the Lr1 resistance gene. In addition, the candidate gene was also confirmed by virus-induced gene silencing. Twenty T 1 lines from resistant transgenic line T 0-938 segregated for resistance, partial resistance and susceptibility to Avr1 corresponding to a 1:2:1 ratio for a single hemizygous insertion. Transgene presence and expression correlated with the phenotype. The resistance phenotype expressed by Lr1 seemed therefore to be dependant on the zygosity status. T 3-938 sister lines with and without the transgene were further tested with 16 virulent and avirulent rust isolates. Rust reactions were all as expected for Lr1 thereby providing additional evidence toward the Lr1 identity of RGA567-5. Sequence analysis of Lr1 indicated that it is not related to the previously isolated Lr10 and Lr21 genes and unlike these genes, it is part of a large gene family.
Plant Molecular Biology – Springer Journals
Published: Jul 5, 2007
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.