Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/wiley/crn-1-a-caenorhabditis-elegans-fen-1-homologue-cooperates-with-cps-6-cjVPsYRxxq
References (36)
-
Jianhua Zhang, Ming Xu (2002)
Apoptotic DNA fragmentation and tissue homeostasis.Trends in cell biology, 12 2
-
N. Munakata (1989)
[Genetics of Caenorhabditis elegans].Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 34 4
-
Lily Li, Xu Luo, Xiaodong Wang (2001)
Endonuclease G is an apoptotic DNase when released from mitochondriaNature, 412
-
Jay Parrish, Lily Li, K.-P. Klotz, Duncan Ledwich, Xiaodong Wang, D. Xue (2001)
Mitochondrial endonuclease G is important for apoptosis in C. elegansNature, 412
-
Xiaochen Wang, Chonglin Yang, J. Chai, Yigong Shi, D. Xue (2002)
Mechanisms of AIF-Mediated Apoptotic DNA Degradation in Caenorhabditis elegansScience, 298
-
C. Savage, M. Hamelin, J. Culotti, Alan Coulson, D. Albertson, M. Chalfie (1989)
mec-7 is a beta-tubulin gene required for the production of 15-protofilament microtubules in Caenorhabditis elegans.Genes & development, 3 6
-
Stephen Goff (1999)
Introduction of H. Robert HorvitzCancer Research, 59
-
D. Tishkoff, N. Filosi, Gretchen Gaida, R. Kolodner (1997)
A Novel Mutation Avoidance Mechanism Dependent on S. cerevisiae RAD27 Is Distinct from DNA Mismatch RepairCell, 88
-
S. Goff (1999)
Genetic control of programmed cell death in the nematode Caenorhabditis elegans.Cancer research, 59 7 Suppl
-
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual -
Jay Parrish, Helen Metters, Lin Chen, D. Xue (2000)
Demonstration of the in vivo interaction of key cell death regulators by structure-based design of second-site suppressors.Proceedings of the National Academy of Sciences of the United States of America, 97 22
-
M. Enari, H. Sakahira, H. Yokoyama, K. Okawa, A. Iwamatsu, S. Nagata (1998)
A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICADNature, 391
-
B. Shen, J. Nolan, L. Sklar, Min-Seon Park (1996)
Essential Amino Acids for Substrate Binding and Catalysis of Human Flap Endonuclease 1 (*)The Journal of Biological Chemistry, 271
-
J. Nolan, B. Shen, M. Park, L. Sklar (1996)
Kinetic analysis of human flap endonuclease-1 by flow cytometry.Biochemistry, 35 36
-
Christelle Gally, J. Bessereau (2003)
[C. elegans: of neurons and genes].Medecine sciences : M/S, 19 6-7
-
John Harrington, Michael Lieber (1994)
The characterization of a mammalian DNA structure‐specific endonuclease.The EMBO Journal, 13
-
E. Vallen, F. Cross (1995)
Mutations in RAD27 define a potential link between G1 cyclins and DNA replicationMolecular and Cellular Biology, 15
-
M. Lieber (1997)
The FEN‐1 family of structure‐specific nucleases in eukaryotic dna replication, recombination and repairBioEssays, 19
-
D. Hosfield, C. Mol, B. Shen, J. Tainer (1998)
Structure of the DNA Repair and Replication Endonuclease and Exonuclease FEN-1 Coupling DNA and PCNA Binding to FEN-1 ActivityCell, 95
-
X. Liu, Hua Zou, C. Slaughter, Xiaodong Wang (1997)
DFF, a Heterodimeric Protein That Functions Downstream of Caspase-3 to Trigger DNA Fragmentation during ApoptosisCell, 89
-
G. Stanfield, H. Horvitz (2000)
The ced-8 gene controls the timing of programmed cell deaths in C. elegans.Molecular cell, 5 3
-
Jay Parrish, D. Xue (2003)
Functional genomic analysis of apoptotic DNA degradation in C. elegans.Molecular cell, 11 4
-
Andrew Smith (1998)
Genome sequence of the nematode C-elegans: A platform for investigating biologyScience, 282
-
R. Bambara, R. Murante, L. Henricksen (1997)
Enzymes and Reactions at the Eukaryotic DNA Replication Fork*The Journal of Biological Chemistry, 272
-
Y. Shibata, Takashi Nakamura (2002)
Defective Flap Endonuclease 1 Activity in Mammalian Cells Is Associated with Impaired DNA Repair and Prolonged S Phase Delay*The Journal of Biological Chemistry, 277
-
Kyung-Hwa Kim, S. Biade, Y. Matsumoto (1998)
Involvement of Flap Endonuclease 1 in Base Excision DNA Repair*The Journal of Biological Chemistry, 273
-
A. Wyllie (1980)
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activationNature, 284
-
Dorian McIlroy, Masato Tanaka, H. Sakahira, H. Fukuyama, Misao Suzuki, Ken Yamamura, Yoshiyuki Ohsawa, Yasuo Uchiyama, Shigekazu Nagata (2000)
An auxiliary mode of apoptotic DNA fragmentation provided by phagocytes.Genes & development, 14 5
-
Xiangyang Li, Jun Li, J. Harrington, M. Lieber, P. Burgers (1995)
Lagging Strand DNA Synthesis at the Eukaryotic Replication Fork Involves Binding and Stimulation of FEN-1 by Proliferating Cell Nuclear Antigen (*)The Journal of Biological Chemistry, 270
-
Xuesong Liu, Peng Li, Peng Li, P. Widłak, P. Widłak, Hua Zou, Xu Luo, W. Garrard, Xiaodong Wang (1998)
The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis.Proceedings of the National Academy of Sciences of the United States of America, 95 15
-
M. Reagan, Christopher Pittenger, W. Siede, E. Friedberg (1995)
Characterization of a mutant strain of Saccharomyces cerevisiae with a deletion of the RAD27 gene, a structural homolog of the RAD2 nucleotide excision repair geneJournal of Bacteriology, 177
-
C. Mello, J. Kramer, D. Stinchcomb, V. Ambros (1991)
Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.The EMBO Journal, 10
-
CRN-1 and CPS-6 promote apoptotic DNA degradation -
P. Widłak, Lily Li, Xiaodong Wang, W. Garrard (2001)
Action of Recombinant Human Apoptotic Endonuclease G on Naked DNA and Chromatin SubstratesThe Journal of Biological Chemistry, 276
-
E. Vallen, F. Cross (1995)
Mutations in RAD27 Define a Potential Link between G 1 Cyclins and DNA Replication -
Yi-Chun Wu, G. Stanfield, H. Horvitz (2000)
NUC-1, a caenorhabditis elegans DNase II homolog, functions in an intermediate step of DNA degradation during apoptosis.Genes & development, 14 5
- Publisher
- Wiley
- Copyright
- Copyright © 2013 Wiley Periodicals, Inc
- ISSN
- 0261-4189
- eISSN
- 1460-2075
- DOI
- 10.1093/emboj/cdg320
- pmid
- 12840007
- Publisher site
- See Article on Publisher Site
Abstract
Oligonucleosomal fragmentation of chromosomes in dying cells is a hallmark of apoptosis. Little is known about how it is executed or what cellular components are involved. We show that crn‐1, a Caenorhabditis elegans homologue of human flap endonuclease‐1 (FEN‐1) that is normally involved in DNA replication and repair, is also important for apoptosis. Reduction of crn‐1 activity by RNA interference resulted in cell death phenotypes similar to those displayed by a mutant lacking the mitochondrial endonuclease CPS‐6/endonuclease G. CRN‐1 localizes to nuclei and can associate and cooperate with CPS‐6 to promote stepwise DNA fragmentation, utilizing the endonuclease activity of CPS‐6 and both the 5′–3′ exonuclease activity and a previously uncharacterized gap‐dependent endonuclease activity of CRN‐1. Our results suggest that CRN‐1/FEN‐1 may play a critical role in switching the state of cells from DNA replication/repair to DNA degradation during apoptosis.
Journal
The EMBO Journal – Wiley
Published: Jan 1, 2003
Keywords: ; ; ; ;