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cDNA cloning and expression of a gene for 3‐ketoacyl‐CoA thiolase in pumpkin cotyledons.Plant and Cell Physiology, 31
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Targeting of human catalase to peroxisomes is dependent upon a novel COOH-terminal peroxisomal targeting sequenceThe Journal of Cell Biology, 134
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Rat liver catalase is sorted to peroxisomes by its C-terminal tripeptide Ala-Asn-Leu, not by the internal Ser-Lys-Leu motif.European journal of cell biology, 71 3
(2003)
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Structure and regulated expression of genes encoding fructose biphosphate aldolase in Trypanosoma brucei.The EMBO Journal, 4
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M.S. Lee, R.T. Mullen, R.N. Trelease (1997)
Oilseed isocitrate lyases lacking their essential type 1 peroxisomal targeting signal are piggybacked to glyoxysomes.J. Cell. Biol., 9
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J.A. McNew, J.M. Goodman (1994)
An oligomeric protein is imported into peroxisomes in vivo.Trends Biol. Sci., 127
Y. Elgersma, C.W. Van Roermund, R.J. Wanders, H.F. Tabak (1995)
Peroxisomal and mitochondrial carnitine acetyltransferases of Saccharomyces cerevisiae are encoded by a single gene.J. Biol. Chem., 14
Bernhard Wimmer, Friedrich Lottspeich, Van Klei, Marten Veenhuis, C. Gietl (1997)
The glyoxysomal and plastid molecular chaperones (70-kDa heat shock protein) of watermelon cotyledons are encoded by a single gene.Proceedings of the National Academy of Sciences of the United States of America, 94 25
Y. Elgersma, H.F. Tabak (1996)
Proteins involved in peroxisome biogenesis and functioning.EMBO J., 1286
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Identification of the peroxisomal targeting signal for cottonseed catalase.The Plant journal : for cell and molecular biology, 12 2
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Characterization of the signal peptide at the amino terminus of the rat peroxisomal 3-ketoacyl-CoA thiolase precursor.The Journal of biological chemistry, 269 8
E. Middelkoop, A. Strijland, J. Tager (1991)
Does aminotriazole inhibit import of catalase into peroxisomes by retarding unfolding?FEBS Letters, 279
A. Kermode (1996)
Mechanisms of Intracellular Protein Transport and Targeting in Plant CellsCritical Reviews in Plant Sciences, 15
Christine Gietl (1990)
Glyoxysomal malate dehydrogenase from watermelon is synthesized with an amino-terminal transit peptide.Proceedings of the National Academy of Sciences of the United States of America, 87
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Import of DHFR hybrid protein into glycosomes in vivo is not inhibited by the folate‐analogue aminopterin.J. Biol. Chem., 132
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Amino‐terminal presequence of the precursor of peroxisomal 3‐ketoacyl‐CoA thiolase is a cleavable signal peptide for peroxisomal targeting.Plant Mol. Biol., 181
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Mutational analysis of the N-terminal topogenic signal of watermelon glyoxysomal malate dehydrogenase using the heterologous host Hansenula polymorpha.Proceedings of the National Academy of Sciences of the United States of America, 91
J. Horng, R. Behari, L. Burke, A. Baker (1995)
Investigation of the energy requirement and targeting signal for the import of glycolate oxidase into glyoxysomes.European journal of biochemistry, 230 1
T. Kurihara, M. Ueda, N.J. Kanayama, Y. Teranishi, A. Tanaka (1992)
Peroxisomal acetoacetyl‐CoA thiolase of an n‐alkane‐utilizing yeast, Candida tropicalis.J. Cell Biol., 210
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Targeting and processing of a chimeric protein with the N‐terminal presequence of the precursor to glyoxysomal citrate synthase.Plant Mol. Biol., 8
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Import of a DHFR hybrid protein into glycosomes in vivo is not inhibited by the folate-analogue aminopterinThe Journal of Cell Biology, 132
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Identification of PAHX, a Refsum disease gene.J. Cell Biol., 17
A. Kato, T. Yoshikawa, M. Hayashi, M. Kondo, I. Nishimura, M. Nishimura (1998)
Glyoxysomal malate dehydrogenase in pumpkin: Cloning of a cDNA and functional analysis of its presequence.Crit. Rev. Plant Sci., 9
Alison Motley, Michael Lumb, P. Oatey, Patricia Jennings, P. Zoysa, Ronald Anders, H. Tabak, C. Danpure (1995)
Mammalian alanine/glyoxylate aminotransferase 1 is imported into peroxisomes via the PTS1 translocation pathway. Increased degeneracy and context specificity of the mammalian PTS1 motif and implications for the peroxisome-to-mitochondrion mistargeting of AGT in primary hyperoxaluria type 1The Journal of Cell Biology, 131
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Conservative amino acid substitutions of the C-terminal tripeptide (Ala-Arg-Met) on cottonseed isocitrate lyase preserve import in vivo into mammalian cell peroxisomes.European journal of cell biology, 65 2
R.T. Mullen, M.S. Lee, R.N. Trelease (1997b)
Identification of the peroxisomal targeting signal for cottonseed catalase.Mol. Cell Biol., 12
T. Osumi, T. Tsukamoto, S. Hata, S. Yokota, S. Miura, Y. Fujiki, M. Hijikata, S. Miyazawa, T. Hashimoto (1991)
Amino-terminal presequence of the precursor of peroxisomal 3-ketoacyl-CoA thiolase is a cleavable signal peptide for peroxisomal targeting.Biochemical and biophysical research communications, 181 3
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Molecular Characterization of a Glyoxysomal Long Chain Acyl-CoA Oxidase That Is Synthesized as a Precursor of Higher Molecular Mass in Pumpkin*The Journal of Biological Chemistry, 273
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Purification and biosynthesis of cottonseed (Gossypium hirsutum L.) catalase.The Biochemical journal, 251 1
C. Kruse, H. Kindl (1983)
Malate synthase: aggregation, deaggregation, and binding of phospholipids.Archives of biochemistry and biophysics, 223 2
R. Rachubinski, S. Subramani (1995)
How proteins penetrate peroxisomesCell, 83
J.M. Leiper, P.B. Oatey, C.J. Danpure (1996)
Inhibition of alanine: glyoxylate aminotransferase 1 dimerization is a prerequisite for its peroxisome‐to‐mitochondrion mistargeting in primary hyperoxaluria type 1.J. Mol. Biol., 135
Fritz Kragler, Alfred Langeder, Jana Raupachova, M. Binder, A. Hartig (1993)
Two independent peroxisomal targeting signals in catalase A of Saccharomyces cerevisiaeThe Journal of Cell Biology, 120
D.J. Kim, S.M. Smith (1994)
Expression of a single gene encoding microbody NAD‐malate dehydrogenase during glyoxysome and peroxisome development in cucumber.J. Cell Biol., 26
G. Berninger, R. Schmidtchen, G. Casel, Rautenstrauss Knörr, W.‐H. Kunau, E. Schweizer (1993)
Structure and metabolic control of the Yarrowia lipolytica peroxisomal 3‐oxoacyl‐CoA‐thiolase gene.FEBS Lett., 216
R. Mullen, M. Lee, C. Flynn, R. Trelease (1997)
Diverse Amino Acid Residues Function within the Type 1 Peroxisomal Targeting Signal (Implications for the Role of Accessory Residues Upstream of the Type 1 Peroxisomal Targeting Signal), 115
Judith Blattner, Heinz Dörsam, Christine Clayton (1995)
Function of N‐terminal import signals in trypanosome microbodiesFEBS Letters, 360
John Glover, David Andrews, S. Subramani, R. Rachubinski (1994)
Mutagenesis of the amino targeting signal of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase reveals conserved amino acids required for import into peroxisomes in vivo.The Journal of biological chemistry, 269 10
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A full‐length cDNA encoding 3‐ketoacyl‐CoA thiolase from Brassica napusBiochem. Biophys. Res. Comm., 110
S. Nye, R. Scarpulla (1990)
In vivo expression and mitochondrial targeting of yeast apoiso-1-cytochrome c fusion proteinsMolecular and Cellular Biology, 10
D. Brickner, L. Olsen (1998)
Nucleotide triphosphates are required for the transport of glycolate oxidase into peroxisomes.Plant physiology, 116 1
C. Gietl, N. Faber, K. van der Klei, J. Ida, M. Veenhuis (1994)
Mutational analysis of the N‐terminal topogenic signal of watermelon glyoxysomal malate dehydrogenase using the heterologous host Hansenula polymorpha. ProceedingsNatl Acad. Sci. USA, 91
R. Preisig‐Muller, H. Kindl (1993)
Thiolase mRNA translated in vitro yields a peptide with a putative N‐terminal presequence.Cell, 22
J. McNew, J. Goodman (1994)
An oligomeric protein is imported into peroxisomes in vivoThe Journal of Cell Biology, 127
B. Swinkels, Stephen Gouldl, A. Bodnar, R. Rachubinski, Suresh Subramanil (1991)
A novel, cleavable peroxisomal targeting signal at the amino‐terminus of the rat 3‐ketoacyl‐CoA thiolase.The EMBO Journal, 10
J Goodman, C Scott, P Donahue, J Atherton (1984)
Alcohol oxidase assembles post-translationally into the peroxisome of Candida boidinii.The Journal of biological chemistry, 259 13
A.R. Kermode (1996)
Mechanisms of intracellular protein transport and targeting in plant cells.Plant Mol. Biol., 15
J. Leiper, P. Oatey, C. Danpure (1996)
Inhibition of alanine:glyoxylate aminotransferase 1 dimerization is a prerequisite for its peroxisome-to-mitochondrion mistargeting in primary hyperoxaluria type 1The Journal of Cell Biology, 135
P.G. Bruinenberg, M. Evers, H.R. Waterham, J. Kuipers, A.C. Arnberg, G. Ab (1989)
Cloning and sequencing of the peroxisomal amine oxidase gene from Hansenula polymorpha.EMBO J., 1008
Michael Passreiter, M. Anton, D. Lay, R. Frank, C. Harter, F. Wieland, K. Gorgas, W. Just (1998)
Peroxisome Biogenesis: Involvement of ARF and CoatomerThe Journal of Cell Biology, 141
In this study of the type 2 peroxisomal targeting signal (PTS2) pathway, we examined the apparent discontinuity and conservation of residues within the PTS2 nonapeptide and demonstrated that this topogenic signal is capable of directing heteromultimeric protein import in plant cells. Based on cumulative data showing that at least 26 unique, putative PTS2 nonapeptides occur within 12 diverse peroxisomal‐destined proteins, the current (‐R/K‐L/V/I‐X5‐H/Q‐L/A‐) as well as the original (‐R‐L‐X5‐H/Q‐L‐) PTS2 motif appear to be oversimplified. To assess the functionality of residues within the motif, rat liver thiolase (rthio) and various chimeric chloramphenicol acetyltransferase (CAT) proteins were expressed transiently in suspension‐cultured tobacco ( Nicotiana tabacum L.) cv Bright Yellow cells (BY‐2), and their subcellular location was determined by immunofluorescence microscopy. Hemagglutinin (HA)‐epitope‐tagged‐CAT subunits, lacking a PTS2 (CAT‐HA), were ‘piggybacked’ into glyoxysomes by PTS2‐bearing CAT subunits (rthio‐CAT), whereas signal‐depleted CAT‐HA subunits that were modified to prevent oligomerization did not import into glyoxysomes. These results provided direct evidence that signal‐depleted subunits imported into peroxisomes were targeted to the organelle as oligomers (heteromers) by a PTS2. Mutational analysis of residues within PTS2 nonapeptides revealed that a number of amino acid substitutions were capable of maintaining targeting function. Furthermore, functionality of residues within the PTS2 nonapeptide did not appear to require a context‐specific environment conferred by adjacent residues. These results collectively suggest that the functional PTS2 is not solely defined as a sequence‐specific motif, i.e. ‐R/K‐X6‐H/Q‐A/L/F‐, but defined also by its structural motif that is dependent upon the physiochemical properties of residues within the nonapeptide.
The Plant Journal – Wiley
Published: Dec 1, 1998
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