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Glucocorticoids Increase Catecholamine Synthesis and Storage in PC 12 Pheochromocytoma Cell Cultures

Glucocorticoids Increase Catecholamine Synthesis and Storage in PC 12 Pheochromocytoma Cell Cultures Abstract: Glucocorticoids, cholera toxin and high plating density all increase the activity of tyrosine 3‐monooxygenase (TH) in cultured PC12 pheochromocytoma cells. Glucocorticoids increase enzyme activity in cells treated with cholera toxin and in cells grown at high plating density. Glucocorticoids also increase the content of stored catecholamines in the cells. In cells cultured under routine conditions, glucocorticoids primarily increase the stores of dopamine. The addition of ascorbate to the culture medium increases the storage of norepinephrine in both steroid‐treated and untreated cells. Incubation of the cells in media containing 56 nM K+ causes the release of the same percentage of stored dopamine from steroid‐treated as from untreated cells. Steroid‐treated cells contain more dopamine than do untreated cells and therefore, in response to high K+, the steroid‐treated cells secrete more dopamine than do untreated cells. We conclude that the activity of tyrosine 3‐monooxygenase in PC12 cells can be regulated by several distinct mechanisms; that glucocorticoids cause a coordinate increase in TH activity and in catecholamine storage; that steroids increase the storage of catecholamines in a releasable pool; and that the steroid‐induced increase in catecholamine storage may result in increased secretion of catecholamines from steroid‐treated cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Glucocorticoids Increase Catecholamine Synthesis and Storage in PC 12 Pheochromocytoma Cell Cultures

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References (33)

Publisher
Wiley
Copyright
Copyright © 1983 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1983.tb11291.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: Glucocorticoids, cholera toxin and high plating density all increase the activity of tyrosine 3‐monooxygenase (TH) in cultured PC12 pheochromocytoma cells. Glucocorticoids increase enzyme activity in cells treated with cholera toxin and in cells grown at high plating density. Glucocorticoids also increase the content of stored catecholamines in the cells. In cells cultured under routine conditions, glucocorticoids primarily increase the stores of dopamine. The addition of ascorbate to the culture medium increases the storage of norepinephrine in both steroid‐treated and untreated cells. Incubation of the cells in media containing 56 nM K+ causes the release of the same percentage of stored dopamine from steroid‐treated as from untreated cells. Steroid‐treated cells contain more dopamine than do untreated cells and therefore, in response to high K+, the steroid‐treated cells secrete more dopamine than do untreated cells. We conclude that the activity of tyrosine 3‐monooxygenase in PC12 cells can be regulated by several distinct mechanisms; that glucocorticoids cause a coordinate increase in TH activity and in catecholamine storage; that steroids increase the storage of catecholamines in a releasable pool; and that the steroid‐induced increase in catecholamine storage may result in increased secretion of catecholamines from steroid‐treated cells.

Journal

Journal of Neurochemistry – Wiley

Published: Feb 1, 1983

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