Access the full text.
Sign up today, get DeepDyve free for 14 days.
Heinrich Cuypers, Adelheid Viebrock-Sambale, W. Zumft (1992)
NosR, a membrane-bound regulatory component necessary for expression of nitrous oxide reductase in denitrifying Pseudomonas stutzeriJournal of Bacteriology, 174
S. Spiro, J. Guest (1990)
FNR and its role in oxygen-regulated gene expression in Escherichia coli.FEMS microbiology reviews, 6 4
Nielsen Nielsen, Engelbrecht Engelbrecht, Brunak Brunak, Vonheijne Vonheijne (1997)
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sitesProtein Eng, 10
Anthonius Boer, J. Oost, W. Reijnders, H. Westerhoff, A. Stouthamer, R. Spanning (1996)
Mutational analysis of the nor gene cluster which encodes nitric-oxide reductase from Paracoccus denitrificans.European journal of biochemistry, 242 3
P. Holloway, W. McCormick, R. Watson, Y. Chan (1996)
Identification and analysis of the dissimilatory nitrous oxide reduction genes, nosRZDFY, of Rhizobium melilotiJournal of Bacteriology, 178
H. Arai, Y. Igarashi, T. Kodama (1994)
Structure and ANR-dependent transcription of the nir genes for denitrification from Pseudomonas aeruginosa.Bioscience, biotechnology, and biochemistry, 58 7
Y. Chan, W. McCormick, R. Watson (1997)
A new nos gene downstream from nosDFY is essential for dissimilatory reduction of nitrous oxide by Rhizobium (Sinorhizobium) meliloti.Microbiology, 143 ( Pt 8)
R. Spanning, C. Wansell, N. Harms, L. Oltmann, A. Stouthamer (1990)
Mutagenesis of the gene encoding cytochrome c550 of Paracoccus denitrificans and analysis of the resultant physiological effectsJournal of Bacteriology, 172
R. Simon, U. Priefer, A. Pühler (1983)
Vector Plasmids for in-Vivo and in-Vitro Manipulations of Gram-Negative Bacteria
Matchová Matchová, Kucera Kucera, Janiczek Janiczek, Van Spanning Van Spanning, Oltmann Oltmann (1993)
The existence of an alternative electron transfer pathway to the periplasmic nitrite reductase (cytochrome cd 1) in Paracoccus denitrificansArch Microbiol, 159
I. Tosques, J. Shi, J. Shapleigh (1996)
Cloning and characterization of nnrR, whose product is required for the expression of proteins involved in nitric oxide metabolism in Rhodobacter sphaeroides 2.4.3Journal of Bacteriology, 178
G. Ditta, S. Stanfield, D. Corbin, D. Helinski (1980)
Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.Proceedings of the National Academy of Sciences of the United States of America, 77 12
Rob Van, C. Wansell, W. Reijnders, N. Harms, J. Ras, L. Oltmann, A. Stouthamer (1991)
A method for introduction of unmarked mutations in the genome of Paracoccus denitrificans: construction of strains with multiple mutations in the genes encoding periplasmic cytochromes c550, c551i, and c553iJournal of Bacteriology, 173
H. Boyer, D. Roulland-Dussoix (1969)
A complementation analysis of the restriction and modification of DNA in Escherichia coli.Journal of molecular biology, 41 3
Bell Bell, Richardson Richardson, Ferguson Ferguson (1990)
Periplasmic and membrane‐bound respiratory nitrate reductases in Thiosphaera pantotropha . The periplasmic enzyme catalyzes the first step in aerobic denitrificationFEBS Lett, 265
Zimmer Zimmer, Danneberg Danneberg, Bothe Bothe (1985)
Amperometric method for determining nitrous oxide in denitrification and in nitrogenase‐catalyzed nitrous oxide reductionCurr Microbiol, 12
S. Altschul, W. Gish, W. Miller, E. Myers, D. Lipman (1990)
Basic local alignment search tool.Journal of molecular biology, 215 3
A. Kwiatkowski, J. Shapleigh (1996)
Requirement of Nitric Oxide for Induction of Genes Whose Products Are Involved in Nitric Oxide Metabolism in Rhodobacter sphaeroides 2.4.3*The Journal of Biological Chemistry, 271
R. Spanning, E. Houben, W. Reijnders, S. Spiro, H. Westerhoff, N. Saunders (1999)
Nitric Oxide Is a Signal for NNR-Mediated Transcription Activation in Paracoccus denitrificansJournal of Bacteriology, 181
B. Berks, S. Ferguson, James Moir, James Moir, David Richardson (1995)
Enzymes and associated electron transport systems that catalyse the respiratory reduction of nitrogen oxides and oxyanions.Biochimica et biophysica acta, 1232 3
A. Kwiatkowski, W. Laratta, A. Toffanin, J. Shapleigh (1997)
Analysis of the role of the nnrR gene product in the response of Rhodobacter sphaeroides 2.4.1 to exogenous nitric oxideJournal of Bacteriology, 179
M. Frohman (1995)
[42] – Rapid Amplification of Complementary DNA Ends for Generation of Full-Length Complementary DNAs: Thermal RACE
De Boer De Boer, Reijnders Reijnders, Kuenen Kuenen, Stouthamer Stouthamer, Van Spanning Van Spanning (1994)
Isolation, sequencing and mutational analysis of a gene cluster involved in nitrite reduction in Paracoccus denitrificansAntonie Van Leeuwenhoek, 66
F. Boogerd, H. Verseveld, A. Stouthamer (1980)
Electron transport to nitrous oxide in Paracoccus denitrificansFEBS Letters, 113
L. Bell, D. Richardson, S. Ferguson (1990)
Periplasmic and membrane‐bound respiratory nitrate reductases in Thiosphaera pantotrophaFEBS Letters, 265
(1995)
Prediction of helical transmembrane segments at 95% accuracy
R. Spanning, A. Boer, W. Reijnders, H. Westerhoff, A. Stouthamer, J. Oost (1997)
FnrP and NNR of Paracoccus denitrificans are both members of the FNR family of transcriptional activators but have distinct roles in respiratory adaptation in response to oxygen limitationMolecular Microbiology, 23
H. Aiba, S. Adhya, B. Crombrugghe (1981)
Evidence for two functional gal promoters in intact Escherichia coli cells.The Journal of biological chemistry, 256 22
H. Arai, Y. Igarashi, T. Kodama (1995)
Expression of the nir and nor genes for denitrification of Pseudomonas aeruginosa requires a novel CRP/FNR‐related transcriptional regulator, DNR, in addition to ANRFEBS Letters, 371
De Vries De Vries, Harms Harms, Hoogendijk Hoogendijk, Stouthamer Stouthamer (1989)
Isolation and characterization of Paracoccus denitrificans mutants with increased conjugation frequencies and pleiotropic loss of a n(GATC)n DNA‐modifying propertyArch Microbiol, 152
Henrik Nielsen, J. Engelbrecht, S. Brunak (1997)
SHORT COMMUNICATION Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites
Schmehl Schmehl, Jahn Jahn, Meyer zu Vilsendorf Meyer zu Vilsendorf, Hennecke Hennecke, Masepohl Masepohl, Schuppler Schuppler (1993)
Identification of a new class of nitrogen fixation genes in Rhodobacter capsulatus : a putative membrane complex involved in electron transport to nitrogenaseMol Gen Genet, 241
E. Gerhus, P. Steinrücke, Bernd Ludwig (1990)
Paracoccus denitrificans cytochrome c1 gene replacement mutantsJournal of Bacteriology, 172
Jeffrey Miller (1972)
Experiments in molecular genetics
(1993)
Current Protocols in Molecular Biology
A. Leeuwenhoek
The FNR family of transcriptional regulators
Zumft Zumft, Dreusch Dreusch, Lochelt Lochelt, Cuypers Cuypers, Friedrich Friedrich, Schneider Schneider (1992)
Derived amino acid sequences of the nosZ gene from Alcaligenes eutrophus, Pseudomonas aeruginosa and Pseudomonas stutzeri reveal potential copper‐binding residuesEur J Biochem, 208
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual
W. Zumft, A. Dreusch, S. Löchelt, H. Cuypers, B. Friedrich, B. Schneider (1992)
Derived amino acid sequences of the nosZ gene (respiratory N2O reductase) from Alcaligenes eutrophus, Pseudomonas aeruginosa and Pseudomonas stutzeri reveal potential copper-binding residues. Implications for the CuA site of N2O reductase and cytochrome-c oxidase.European journal of biochemistry, 208 1
W. Zumft (1997)
Cell biology and molecular basis of denitrification.Microbiology and molecular biology reviews : MMBR, 61 4
M Frohman (1993)
Rapid amplification of complementary DNA ends for generation of full-length complementary DNAs: thermal RACE.Methods in enzymology, 218
R. Spanning, A. Boer, W. Reijnders, S. Spiro, H. Westerhoff, A. Stouthamer, J. Oost (1995)
Nitrite and nitric oxide reduction in Paracoccus denitrificans is under the control of NNR, a regulatory protein that belongs to the FNR family of transcriptional activatorsFEBS Letters, 360
Kessler Kessler, De Lorenzo De Lorenzo, Timmis Timmis (1992)
A general system to integrate lacZ fusions into the chromosomes of Gram‐negative eubacteria; regulation of the Pm promoter of the TOL plasmid studied with all the controlling elements in monocopyMol Gen Genet, 233
Y. Lam, D. Nicholas (1969)
A nitrite reductase with cytochrome oxidase activity from Micrococcus denitrificans.Biochimica et biophysica acta, 180 3
R. Spanning, A. Boer, D. Slotboom, W. Reijnders, A. Stouthamer (1995)
Isolation and characterization of a novel insertion sequence element, IS1248, in Paracoccus denitrificans.Plasmid, 34 1
B. Rost, C. Sander (1994)
Combining evolutionary information and neural networks to predict protein secondary structureProteins: Structure, 19
F. Hoeren, B. Berks, S. Ferguson, J. McCarthy (1993)
Sequence and expression of the gene encoding the respiratory nitrous-oxide reductase from Paracoccus denitrificans. New and conserved structural and regulatory motifs.European journal of biochemistry, 218 1
B. Rost, C. Sander, R. Casadio, P. Fariselli (1995)
Transmembrane helices predicted at 95% accuracyProtein Science, 4
Plumbridge Plumbridge, Kolb Kolb (1995)
Nag repressor–operator interactions: protein–DNA contacts cover more than two turns of the DNA helix (published erratum appears in J Mol Biol 1995 253: 219–220)J Mol Biol, 249
H. Lawford, J. Cox, P. Garland, B. Haddock (1976)
Electron transport in aerobically grown Paracoccus denitrificans: Kinetic characterization of the membrane‐bound cytochromes and the stoichiometry of respiration‐driven proton translocationFEBS Letters, 64
Jacqueline Plumbridge, Annie Kolb (1995)
Nag repressor-operator interactions: protein-DNA contacts cover more than two turns of the DNA helix.Journal of molecular biology, 249 5
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
J. Chang, J. Morris (1962)
Studies on the utilization of nitrate by Micrococcus denitrificans.Journal of general microbiology, 29
P. Thomas, D. Ryan, W. Levin (1976)
An improved staining procedure for the detection of the peroxidase activity of cytochrome P-450 on sodium dodecyl sulfate polyacrylamide gels.Analytical biochemistry, 75 1
The nirIX gene cluster of Paracoccus denitrificans is located between the nir and nor gene clusters encoding nitrite and nitric oxide reductases respectively. The NirI sequence corresponds to that of a membrane‐bound protein with six transmembrane helices, a large periplasmic domain and cysteine‐rich cytoplasmic domains that resemble the binding sites of (4Fe‐4S) clusters in many ferredoxin‐like proteins. NirX is soluble and apparently located in the periplasm, as judged by the predicted signal sequence. NirI and NirX are homologues of NosR and NosX, proteins involved in regulation of the expression of the nos gene cluster encoding nitrous oxide reductase in Pseudomonas stutzeri and Sinorhizobium meliloti. Analysis of a NirI‐deficient mutant strain revealed that NirI is involved in transcription activation of the nir gene cluster in response to oxygen limitation and the presence of N‐oxides. The NirX‐deficient mutant transiently accumulated nitrite in the growth medium, but it had a final growth yield similar to that of the wild type. Transcription of the nirIX gene cluster itself was controlled by NNR, a member of the family of FNR‐like transcriptional activators. An NNR binding sequence is located in the middle of the intergenic region between the nirI and nirS genes with its centre located at position −41.5 relative to the transcription start sites of both genes. Attempts to complement the NirI mutation via cloning of the nirIX gene cluster on a broad‐host‐range vector were unsuccessful, the ability to express nitrite reductase being restored only when the nirIX gene cluster was reintegrated into the chromosome of the NirI‐deficient mutant via homologous recombination in such a way that the wild‐type nirI gene was present directly upstream of the nir operon.
Molecular Microbiology – Wiley
Published: Oct 1, 1999
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.