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S. Chen, R. Kou, C. Wu, Pei-Chi Wang, F. Su (2001)
Mass mortality associated with a Sphaerospora‐like myxosporidean infestation in juvenile cobia, Rachycentron canadum (L.), marine cage cultured in TaiwanJournal of Fish Diseases, 24
M. Imajoh, Takuya Ikawa, S. Oshima (2007)
Characterization of a new fibroblast cell line from a tail fin of red sea bream, Pagrus major, and phylogenetic relationships of a recent RSIV isolate in Japan.Virus research, 126 1-2
SC Chi, JR Shieh, Sj Lin (2003)
Genetic and antigenic analysis of betanodaviruses isolated from aquatic organisms in Taiwan.Diseases of aquatic organisms, 55 3
Chou (1994)
Investigation on the viral diseases of cultured grouper (Epinephelus sp.) and red sea bream (Pagrus major)Reports on Fish Disease Research (XIV), COA Fisheries Series, 46
S. Chi, C. Lo, G. Kou, P Chang, S. Peng, S Chen (1997)
Mass mortalities associated with viral nervous necrosis (VNN) disease in two species of hatchery‐reared grouper, Epinephelus fuscogutatus and Epinephelus akaara (Temminck & Schlegel)Journal of Fish Diseases, 20
C. Wen, C. Lee, C. Wang, Y. Cheng, Huo-qing Huang (2008)
Development of two cell lines from Epinephelus coioides brain tissue for characterization of betanodavirus and megalocytivirus infectivity and propagationAquaculture, 278
P. Rajan, C. López, Lin Han-You, Yang Huey-Lang (2001)
Vibrio alginolyticus infection in cobia (Rachycentron canadum) cultured in TaiwanBulletin of The European Association of Fish Pathologists, 21
Y. Lai, Y. Lai, Joseph John, Cheng-Hui Lin, Cheng-Hui Lin, I. Guo, S. Chen, K. Fang, Chi-Yao Chang (2003)
Establishment of cell lines from a tropical grouper, Epinephelus awoara (Temminck & Schlegel), and their susceptibility to grouper irido- and nodaviruses.Journal of fish diseases, 26 1
V. Parameswaran, R. Shukla, R. Bhonde, A. Hameed (2007)
Development of a Pluripotent ES-like Cell Line from Asian Sea Bass (Lates calcarifer)—An Oviparous Stem Cell Line Mimicking Viviparous ES CellsMarine Biotechnology, 9
C. Uphoff, H. Drexler (2002)
Comparative PCR analysis for detection of mycoplasma infections in continuous cell linesIn Vitro Cellular & Developmental Biology - Animal, 38
E. Marshall (2005)
Will DNA Bar Codes Breathe Life Into Classification?Science, 307
Y. Lai, S. Murali, H. Ju, M. Wu, I. Guo, S. Chen, K. Fang, C. Chang (2000)
Two iridovirus-susceptible cell lines established from kidney and liver of grouper, Epinephelus awoara (Temminck & Schlegel), and partial characterization of grouper iridovirusJournal of Fish Diseases, 23
E. McLean, G. Salze, S. Craig (2008)
PARASITES, DISEASES AND DEFORMITIES OF COBIACroatian journal of fisheries : Ribarstvo, 66
Songlin Chen, Z. Sha, Hanqing Ye (2003)
Establishment of a pluripotent embryonic cell line from sea perch (Lateolabrax japonicus) embryosAquaculture, 218
Song‐lin Chen, G. Ren, Z. Sha, C. Shi (2004)
Establishment of a continuous embryonic cell line from Japanese flounder Paralichthys olivaceus for virus isolation.Diseases of aquatic organisms, 60 3
Q. Qin, T. Wu, T. Jia, A. Hegde, R. Zhang (2006)
Development and characterization of a new tropical marine fish cell line from grouper, Epinephelus coioides susceptible to iridovirus and nodavirus.Journal of virological methods, 131 1
C. Arnold, Jeffrey Kaiser, G. Holt (2002)
Spawning of Cobia Rachycentron canadum in CaptivityJournal of The World Aquaculture Society, 33
R. Ward, T. Zemlak, B. Innes, P. Last, P. Hebert (2005)
DNA barcoding Australia's fish speciesPhilosophical Transactions of the Royal Society B: Biological Sciences, 360
R. Kane, J. Bartley, J. Stothard, Jozef Vercruysse, David Rollinson, V. Southgate (2002)
Application of single strand conformational polymorphism (SSCP) analysis with fluorescent primers for differentiation of Schistosoma haematobium group species.Transactions of the Royal Society of Tropical Medicine and Hygiene, 96 Suppl 1
X. Xia (2000)
Phylogenetic relationship among horseshoe crab species: effect of substitution models on phylogenetic analyses.Systematic biology, 49 1
S. Lapatra (1996)
The use of serological techniques for virus surveillance and certification of finfishAnnual Review of Fish Diseases, 6
Y. Lai, S. Murali, H. Chiu, H. Ju, Y. Lin, S. Chen, I. Guo, K. Fang, C. Chang (2001)
Propagation of yellow grouper nervous necrosis virus (YGNNV) in a new nodavirus‐susceptible cell line from yellow grouper, Epinephelus awoara (Temminck & Schlegel), brain tissueJournal of Fish Diseases, 24
S.F Chang, G. Ngoh, L. Kueh, Q. Qin, C. Chen, T. Lam, Y. Sin (2001)
Development of a tropical marine fish cell line from Asian seabass (Lates calcarifer) for virus isolationAquaculture, 192
V. Parameswaran, R. Shukla, R. Bhonde, A. Hameed (2006)
Establishment of embryonic cell line from sea bass (Lates calcarifer) for virus isolation.Journal of virological methods, 137 2
Chen (2005)
Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus)Aquaculture, 249
P. Hebert, A. Cywinska, S. Ball, Jeremy deWaard (2003)
Biological identifications through DNA barcodesProceedings of the Royal Society of London. Series B: Biological Sciences, 270
A. Villena (2003)
Applications and needs of fish and shellfish cell culture for disease control in aquacultureReviews in Fish Biology and Fisheries, 13
D. Taylor-Robinson, C. Bébéar (1997)
Antibiotic susceptibilities of mycoplasmas and treatment of mycoplasmal infections.The Journal of antimicrobial chemotherapy, 40 5
J. Quan, Z. Zhuang, Jingyao Deng, J. Dai, Ya-ping Zhang (2004)
Phylogenetic Relationships of 12 Penaeoidea Shrimp Species Deduced from Mitochondrial DNA SequencesBiochemical Genetics, 42
Nobuhito Sone, Hiroyuki Toh (1994)
Membrane-bound Bacillus cytochromes c and their phylogenetic position among bacterial class I cytochromes c.FEMS microbiology letters, 122 3
Guang-Zhou Zhou, Zhengqiu Li, X. Yuan, Qi-ya Zhang (2007)
Establishment, Characterization, and Virus Susceptibility of a New Marine Cell Line from Red Spotted Grouper (Epinephelus akaara)Marine Biotechnology, 9
A. Hameed, V. Parameswaran, R. Shukla, I. Singh, A. Thirunavukkarasu, R. Bhonde (2006)
Establishment and characterization of India's first marine fish cell line (SISK) from the kidney of sea bass (Lates calcarifer)Aquaculture, 257
Hebert Hebert, Cywinska Cywinska, Ball Ball, DeWaard DeWaard (2003)
Biological identifications through DNA barcodesJournal and Proceedings of the Royal Society of New South Wales, 270
Zhengshan Zhao, Yuanan Lu (2006)
Establishment and characterization of two cell lines from bluefin trevally Caranx melampygus.Diseases of aquatic organisms, 68 2
S. Tong, Hong Li, H. Miao (1997)
The establishment and partial characterization of a continuous fish cell line FG-9307 from the gill of flounder Paralichthys olivaceusAquaculture, 156
I. Liao, Tingmiao Huang, Wann-Sheng Tsai, Cheng-Ming Hsueh, Sukwoo Chang, E. Leaño (2004)
Cobia culture in Taiwan: current status and problemsAquaculture, 237
V. Parameswaran, V. Ahmed, R. Shukla, R. Bhonde, A. Hameed (2007)
Development and Characterization of Two New Cell Lines from Milkfish (Chanos chanos) and Grouper (Epinephelus coioides) for Virus IsolationMarine Biotechnology, 9
H. Chou, C. Hsu, T. Peng (1998)
Isolation and Characterization of a Pathogenic Iridovirus from Cultured Grouper (Epinephelus sp.) in TaiwanFish Pathology, 33
D. McIntosh, E. Flaño, T. Grayson, M. Gilpin, Brian Austin, Alberto Villena (1997)
Production of putative virulence factors by Renibacterium salmoninarum grown in cell culture.Microbiology, 143 ( Pt 10)
J. Béjar, J. Porta, J. Borrego, M. Alvarez (2005)
The piscine SAF-1 cell line: genetic stability and labelingMarine Biotechnology, 7
Establishment and characterization of two cobia, Rachycentron canadum, cell lines derived from cobia brain (CB) and cobia fin (CF) are described. Caudal fin and brain from juvenile cobia were dissociated for 30 and 10 min, respectively, in phosphate‐buffered saline containing 0.25% trypsin at 25 °C. The optimal culture condition for both dissociated cells (primary cell culture) was at 28 °C in Leibovitz‐15 medium containing 10% foetal bovine serum. The cells have been sub‐cultured at a ratio of 1:2 for more than 160 passages over a period of 3 years. Origin of the cultured cells was verified by comparison of their sequences of mitochondrial cytochrome oxidase subunit I genes (cox I) with the cox 1 sequence from cobia muscle tissue. The cell lines showed polyploidy. No mycoplasma contamination was detected. Susceptibility to grouper iridovirus was observed for the CB cell line but not the CF cell line. Both cell lines expressed green fluorescent protein after being transfected with green fluorescent reporter gene driven by the cytomegalovirus promoter.
Journal of Fish Diseases – Wiley
Published: Feb 1, 2010
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