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J. Sambrook, E.F. Fritsch, T. Maniatis (1989)
A Labaratory Manual, 2nd Edn.Carbohydr. Res.
M.J.M. Ebskamp, I.M. Van Der Merr, B.A. Sprionk, P.J. Weisbeek, S.J.M. Smeekens (1994)
Accumulation of fructose polymers in transgenic tobacco.New Phytol., 12
N. Sprenger, K. Bortlik, A. Brandt, T. Boller, A. Wiemken (1995)
Purification, cloning, and functional expression of sucrose: fructan 6‐fructosyltransferase, a key enzyme of fructan synthesis in barley. ProceedingsPlant Physiol., 92
E. Klann, S. Yelle, A.B. Bennett (1992)
Tomato fruit acid invertase complementary DNA.Plant Physiol., 99
R. Horsch, J. Fry, N. Hoffman, D. Eichholtz, S. Rogers, R. Fraley (1985)
A simple and general method for transferring genes into plants.Science, 227 4691
A.J. Koops, H.H. Jonker (1994)
Purification and characterization of the enzymes of fructan biosynthesis in tubers of Helianthus tuberosus‘Colombia’. I. Fructan: fructan 1‐fructosyltransferase.Plant Cell, 45
S. Huber (1989)
Biochemical Mechanism for Regulation of Sucrose Accumulation in Leaves during Photosynthesis.Plant physiology, 91 2
Charles Strom (1988)
A three allele restriction fragment length polymorphism within the human Col2A1 gene.Nucleic acids research, 16 18
J. Edelman, T.G. Jefford (1968)
The mechanism of fructosan metabolism in higher plants as exemplified in Helianthus tuberosus.Transgen. Res., 67
N. Sprenger, L. Schellenbaum, K. Van Dun, T. Boller, A. Wiemken (1996)
Fructan synthesis in transgenic tobacco and chicory plants expressing barley sucrose: fructan 6‐fructosyltransferase.Z. Pflanzenphysiol., 400
A. Koops, H. Jonker (1996)
Purification and Characterization of the Enzymes of Fructan Biosynthesis in Tubers of Helianthus tuberosus Colombia (II. Purification of Sucrose:Sucrose 1-Fructosyltransferase and Reconstitution of Fructan Synthesis in Vitro with Purified Sucrose:Sucrose 1-Fructosyltransferase and Fructan:Fructan 1-, 110
C. Unger, M. Hardegger, S. Lienhard, A. Sturm (1994)
cDNA Cloning of Carrot (Daucus carota) Soluble Acid [beta]-Fructofuranosidases and Comparison with the Cell Wall Isoenzyme, 104
M. Bevan (1984)
Binary Agrobacterium vectors for plant transformation.New Phytol., 12
W. Wagner, F. Keller, A. Wiemken (1983)
Fructan Metabolism in Cereals: Induction in Leaves and Compartmentation in Protoplasts and VacuolesZeitschrift für Pflanzenphysiologie, 112
N. Sprenger, K. Bortlik, A. Brandt, T. Boller, A. Wiemken (1995)
Purification, cloning, and functional expression of sucrose:fructan 6-fructosyltransferase, a key enzyme of fructan synthesis in barley.Proceedings of the National Academy of Sciences of the United States of America, 92 25
C.W.E. Darwen, P. John (1989)
Localization of the enzymes of fructan metabolism in vacuoles isolated by a mechanical method from tubers of Jerusalem artichoke (Helianthus tuberosus L.).Bio/Technology, 89
V.A. Reddy, F. Maley (1990)
Identification of an active‐site residue in yeast invertase by affinity labeling and site‐directed mutagenesis.Natl Acad. Sci. USA, 265
J. Pen, L. Molendijk, W. Quax, P. Sijmons, A. Ooyen, P. Elzen, K. Rietveld, A. Hoekema (1992)
Production of Active Bacillus licheniformis Alpha-Amylase in Tobacco and its Application in Starch LiquefactionBio/Technology, 10
R. Höfgen, L. Willmitzer (1988)
Storage of competent cells for Agrobacterium transformation.Nucleic acids research, 16 20
P. Dey, R. Dixon (1985)
Biochemistry of Storage Carbohydrates in Green Plants
Agamia E.H. (1984)
Effects of some post harvest treatments on artichoke heads.Beitr. Tropisch. Landwirtsch. Veterinarmed., 22
F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl (1994)
Current Protocols in Molecular BiologyNucl. Acids Res.
Robert Sévenier, R. Hall, I. Meer, Hanny Hakkert, A. Tunen, A. Koops (1998)
High level fructan accumulation in a transgenic sugar beetNature Biotechnology, 16
A. Koops, H. Jonker (1994)
Purification and characterization of the enzymes of fructan biosynthesis in tubers of Helianthus tuberosus ‘Colombia’ I. Fructan: fructan fructosyl transferaseJournal of Experimental Botany, 45
S.C. Huber (1989)
Biochemical mechanism for regulation of sucrose accumulation in leaves during photosynthesis.J. Exp. Bot., 9
J. Rosenfeld, J. Capdevielle, J. Guillemot, P. Ferrara (1992)
In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis.Analytical biochemistry, 203 1
Norbert Sprenger, Luisa Schellenbaum, K. Dun, T. Boller, A. Wiemken (1997)
Fructan synthesis in transgenic tobacco and chicory plants expressing barley sucrose:fructan 6‐fructosyltransferaseFEBS Letters, 400
I.M. Van Der Meer, M.J.M. Ebskamp, R.G.J. Visser, P.J. Weisbeek, J.C.M. Smeekens (1994)
Fructan as a new carbohydrate sink in transgenic potato plants.Bio/Technology, 6
C.J. Nelson, D. Smith (1986)
Fructans: their nature and occurrence.Annu. Rev. Plant Physiol. Plant Mol. Biol., 5
J. Rosenfeld, J. Capdevielle, J.C. Guillemot, P. Ferrara (1992)
In‐gel digestion of proteins for internal sequence analysis after one‐ or two‐ dimensional gel electrophoresis.FEBS Lett., 203
J. Edelman, T. Jeeeord (1968)
THE MECHANISIM OF FRUCTOSAN METABOLISM IN HIGHER PLANTS AS EXEMPLIFIED IN HELIANTHUS TUBEROSUSNew Phytologist, 67
V. Reddy, F. Maley (1990)
Identification of an active-site residue in yeast invertase by affinity labeling and site-directed mutagenesis.The Journal of biological chemistry, 265 19
Ingrid Meer, M. Ebskamp, Richard Visser, P. Weisbeek, S. Smeekens (1994)
Fructan as a New Carbohydrate Sink in Transgenic Potato Plants.The Plant cell, 6
E. Klann, S. Yelle, A. Bennett (1992)
Tomato fruit Acid invertase complementary DNA : nucleotide and deduced amino Acid sequences.Plant physiology, 99 1
M. Ebskamp, I. Meer, B. Spronk, P. Weisbeek, S. Smeekens (1994)
Accumulation of Fructose Polymers in Transgenic TobaccoBio/Technology, 12
A. Sturm, M. Chrispeels (1990)
cDNA cloning of carrot extracellular beta-fructosidase and its expression in response to wounding and bacterial infection.The Plant cell, 2
R. Sévenier, R. Hall, I.M. Van Der Meer, J.C. Hakkert, A.J. Van Tunen, A.J. Koops (1998)
Fructan beet.Plant Cell
A.J. Cairns (1993)
Evidence for the de novo synthesis of fructan by enzymes from higher plants: a reappraisal of the SST/FFT model.Plant Physiol., 123
M. Bevan (1984)
Binary Agrobacterium vectors for plant transformation.Nucleic acids research, 12 22
F. Ausubel (1989)
Short protocols in molecular biology : a compendium of methods from Current protocols in molecular biology
F.A. Van Engelen, J.W. Molthoff, A.J. Conner, J.‐P. Nap, A. Pereira, W.J. Stiekema (1995)
pBINPLUS: an improved plant transformation vector based on pBIN19.New Phytol., 4
A. Fuchs (1993)
Science and Technology of FructansNucl. Acids Res.
A. Straathof, A. Kieboom, H. Bekkum (1986)
Invertase-catalysed fructosyl transfer in concentrated solutions of sucrose.Carbohydrate research, 146 1
G.A.F. Hendry (1993)
Evolutionary origins and natural functions of fructans – a climatological, biogeographic and mechanistic appraisal.Science, 123
R. Höfgen, L. Willmitzer (1988)
Storage of competent cells for Agrobacterium transformation.Plant Physiol., 16
S. Hendry, L. Boddy, D. Lonsdale (1993)
Interactions between callus cultures of European beech, indigenous ascomycetes and derived fungal extracts.The New phytologist, 123 3
C. Darwen, P. John (1989)
Localization of the Enzymes of Fructan Metabolism in Vacuoles Isolated by a Mechanical Method from Tubers of Jerusalem Artichoke (Helianthus tuberosus L.).Plant physiology, 89 2
C. Pollock, A. Cairns (1991)
Fructan metabolism in grasses and cereals, 42
To study the regulation of fructan synthesis in plants, we isolated two full‐size cDNA clones encoding the two enzymes responsible for fructan biosynthesis in Jerusalem artichoke ( Helianthus tuberosus ): 1‐sucrose:sucrose fructosyl transferase (1‐SST) and 1‐fructan:fructan fructosyl transferase (1‐FFT). Both enzymes have recently been purified to homogeneity from Jerusalem artichoke tubers (Koops and Jonker (1994) J. Exp. Bot. 45, 1623–1631; Koops and Jonker (1996) Plant Physiol. 110, 1167–1175) and their amino acid sequences have been partially determined. Using RT–PCR and primers based on these sequences, specific fragments of the genes were amplified from tubers of Jerusalem artichoke. These fragments were used as probes to isolate the cDNAs encoding 1‐SST and 1‐FFT from a tuber‐specific λZAP library. The deduced amino acid sequences of both cDNAs perfectly matched the sequences of the corresponding purified proteins. At the amino acid level, the cDNA sequences showed 61% homology to each other and 59% homology to tomato vacuolar invertase. Based on characteristics of the deduced amino acid sequence, the first 150 bp of both genes encode a putative vacuolar targeting signal. Southern blot hybridization revealed that both 1‐SST and 1‐FFT are likely to be encoded by single‐copy genes. Expression studies based on RNA blot analysis showed organ‐specific and developmental expression of both genes in growing tubers. Lower expression was detected in flowers and in stem. In other organs, including leaf, roots and dormant tubers, no expression could be detected. In tubers, the spatial and developmental expression correlates with the accumulation of fructans. Using the 1‐sst and 1‐fft cDNAs, chimeric genes were constructed driven by the CaMV 35S promoter. Analysis of transgenic petunia plants carrying these constructs showed that both cDNAs encode functional fructosyltransferase enzymes. Plants transformed with the 35S‐ 1‐sst construct accumulated the oligofructans 1‐kestose (GF2), 1,1‐nystose (GF3) and 1,1,1‐fructosylnystose (GF4). Plants transformed with the 35S‐ 1‐fft construct did not accumulate fructans, probably because of the absence of suitable substrates for 1‐FFT, i.e. fructans with a degree of polymerization ≥ 3 (GF2, GF3, etc.). Nevertheless, protein extracts from these transgenic plants were able to convert GF3, when added as a substrate, into fructans with a higher degree of polymerization. Progeny of crosses between a 35S‐ 1‐sst ‐containing plant and a 35S‐ 1‐fft‐ containing plant, showed accumulation of high‐molecular‐weight fructans in old, senescent leaves. Based on the comparison of the predicted amino acid sequences of 1‐sst and 1‐fft with those of other plant fructosyl transferase genes, we postulate that both plant fructan genes have evolved from plant invertase genes.
The Plant Journal – Wiley
Published: Aug 1, 1998
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