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O. Linhart, D. Gela, M. Flajšhans, P. Duda, M. Rodina, V. Novak (2000)
Alcalase enzyme treatment for elimination of egg stickiness in tench, Tinca tinca L.Aquaculture, 191
Linhart (1995)
Biology of gametes and artificial reproduction in common tench (Tinca tinca L.)Review. Pol. Arch. Hydrobiol., 42
O. Linhart, Ludĕk Šĕtch, Ján Svarc, M. Rodina, Jean Audebert, J. Grecu, R. Billard (2002)
The culture of the European catfish, Silurus glanis, in the Czech Republic and in FranceAquatic Living Resources, 15
Summary The experiment showed different results after a short (2 min) enzyme alcalase Merck EC 3.4.21.14 (5.0 ml L−1 concentration) treatment of tench eggs in contrast to the traditional methods of eliminating egg stickiness involving milk solution (50 g L−1) treatment for 70 min followed by the addition of a talc suspension (33 g L−1) for 10 min or treatment by fine clay suspension (20 g L−1) for 60 min or talc suspension (33 g L−1) for 80 min. The alcalase enzyme treatment resulted in decreased egg stickiness compared with the conventional milk/clay/talc treatments, indicated by lower duration of egg incubation and higher hatching rates (anova for hatching rate, P < 0.0084). The highest hatching rate (93.2%) was achieved using the enzyme; the lowest (31.3%) was using a talc suspension (control hatching rate was 86.2%). Duration of egg incubation at degree‐days (D°) after enzyme treatment (58.6 D°) was about 4–5 h shorter than the classical method using milk solution and talc suspension (63–65 D°). Prolongation in the latter classical method may also be explained by a hardening of the egg envelopes.
Journal of Applied Ichthyology – Wiley
Published: Jun 1, 2003
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