Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/springer-journals/expression-of-a-fusion-protein-containing-human-epidermal-growth-ZCkDdBzHUH
References (19)
-
M. Date, H. Itaya, H. Matsui, Y. Kikuchi (2006)
Secretion of human epidermal growth factor by Corynebacterium glutamicumLetters in Applied Microbiology, 42
-
J. Lee, G. Kim, J. Lee, H. Jun, J. Yu, I. Kong (1998)
Isolation and sequence analysis of metalloprotease gene from Vibrio mimicus.Biochimica et biophysica acta, 1384 1
-
Jia Yin, Keping Xu, Jing Zhang, Ashok Kumar, F. Yu (2007)
Wound-induced ATP release and EGF receptor activation in epithelial cellsJournal of Cell Science, 120
-
CM Overall (2001)
Matrix metalloproteinase substrate binding domains, modules and exosites. Overview and experimental strategiesMethods Mol Biol, 151
-
S. Werner, R. Grose (2003)
Regulation of wound healing by growth factors and cytokines.Physiological reviews, 83 3
-
J. Schlessinger, A. Schreiber, A. Levi, I. Lax, T. Libermann, Y. Yarden (1983)
Regulation of cell proliferation by epidermal growth factor.CRC critical reviews in biochemistry, 14 2
-
A. Sahasrabuddhe, Neesar Ahmed, M. Krishnasastry (2006)
Stress-induced phosphorylation of caveolin-1 and p38, and down-regulation of EGFr and ERK by the dietary lectin jacalin in two human carcinoma cell lines, 11
-
Yi-lin Liu, Liangzhu Huang, Wen-Po Lin, Chung-Chin Tsai, T. Lin, Yu-hua Hu, Hquan-Shin Chen, Jun-Ming Han, H. Wang, Yu-Tien Liu (2006)
Secretion of biologically active human epidermal growth factor from Escherichia coli using Yersinia pestis Caf1 signal peptide.Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi, 39 5
-
T. Ishikawa, H. Terai, T. Kitajima (2001)
Production of a biologically active epidermal growth factor fusion protein with high collagen affinity.Journal of biochemistry, 129 4
-
C. Overall (2001)
Matrix MetaIIoproteinase Substrate Binding Domains, Modules and ExositesMethods of Molecular Biology, 151
-
S. Nakagawa, S. Yoshida, Y. Hirao, S. Kasuga, T. Fuwa (1985)
Biological effects of biosynthetic human EGF on the growth of mammalian cells in vitro.Differentiation; research in biological diversity, 29 3
-
T. Ishikawa, H. Terai, Takashi Yamamoto, K. Harada, T. Kitajima (2003)
Delivery of a growth factor fusion protein having collagen-binding activity to wound tissues.Artificial organs, 27 2
-
Jong-Hee Lee, Sun-Hee Ahn, Eun-Mi Lee, Young-Ok Kim, Sang‐Jun Lee, I. Kong (2003)
Characterization of the enzyme activity of an extracellular metalloprotease (VMC) from Vibrio mimicus and its C-terminal deletions.FEMS microbiology letters, 223 2
-
Seung-Yeol Shin, Jong-Hee Lee, S. Huh, Young-Seo Park, Jin-Man Kim, I. Kong (2000)
Overexpression and characterization of Vibrio mimicus metalloproteaseJournal of Microbiology and Biotechnology, 10
-
Jong-Hee Lee, Sun-Hee Ahn, Eun-Mi Lee, Seung-Ha Jeong, Young-Ok Kim, Sang‐Jun Lee, I. Kong (2005)
The FAXWXXT motif in the carboxyl terminus of Vibrio mimicus metalloprotease is involved in binding to collagenFEBS Letters, 579
-
J. Imanishi, K. Kamiyama, I. Iguchi, M. Kita, C. Sotozono, S. Kinoshita (2000)
Growth factors: importance in wound healing and maintenance of transparency of the corneaProgress in Retinal and Eye Research, 19
-
J. Hong, H. Jung, Y. Kim (2006)
Recombinant Human Epidermal Growth Factor (EGF) to Enhance Healing for Diabetic Foot UlcersAnnals of Plastic Surgery, 56
-
Xin'ai Chen, P. Cen, Jishuang Chen (2005)
Enhanced production of human epidermal growth factor by a recombinant Escherichia coli integrated with in situ exchange of acetic acid by macroporous ion-exchange resin.Journal of bioscience and bioengineering, 100 5
-
N. Nishi, O. Matsushita, K. Yuube, H. Miyanaka, A. Okabe, F. Wada (1998)
Collagen-binding growth factors: production and characterization of functional fusion proteins having a collagen-binding domain.Proceedings of the National Academy of Sciences of the United States of America, 95 12
- Publisher
- Springer Journals
- Copyright
- Copyright © 2008 by Springer Science+Business Media B.V.
- Subject
- Life Sciences; Biochemistry, general; Applied Microbiology; Biotechnology; Microbiology
- ISSN
- 0141-5492
- eISSN
- 1573-6776
- DOI
- 10.1007/s10529-008-9863-6
- pmid
- 18931974
- Publisher site
- See Article on Publisher Site
Abstract
Human epidermal growth factor (hEGF) is a polypeptide of 53 amino acids, is an important autocrine/paracrine factor in the human body, and is used in the pharmaceutical and cosmetics industries. We constructed a fusion hEGF protein with a collagen-binding domain (CBD) composed of 33 amino acids from Vibrio mimicus metalloprotease (VMCBD). The CBD segment of the metalloprotease was fused at the C terminus of the hEGF protein. The recombinant fusion protein was expressed in Escherichia coli and purified. The purified hEGF protein promoted greater growth of human/A-431 cells than did the control hEGF. The fusion EGF protein also showed collagen-binding activity with type I collagen. In contrast, hEGF did not bind to type I collagen. These results suggest that recombinant hEGF protein fused to VMCBD may be able to remain for a long period at injured epidermal tissue acting as a healing agent.
Journal
Biotechnology Letters – Springer Journals
Published: Oct 19, 2008