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- Publisher
- Springer Journals
- Copyright
- Copyright © 2008 by Springer Science+Business Media, LLC
- Subject
- Medicine & Public Health; Pathology ; Pharmacology/Toxicology ; Internal Medicine ; Rheumatology
- ISSN
- 0360-3997
- eISSN
- 1573-2576
- DOI
- 10.1007/s10753-008-9087-x
- pmid
- 18850260
- Publisher site
- See Article on Publisher Site
Abstract
The present study aimed at characterizing the phenotype and functions of adherent synovial fluid (SF) cells derived from rheumatoid arthritis (RA), comparing with fibroblast-like synoviocytes (FLS) derived from RA synovial tissue (ST). Adherent SF-derived cells were spindle-shaped from passages 1–6 under light microscopy. The cell surface marker profile in SF-derived cells from passage 1–6 was similar to that of ST-derived FLS. Levels of MMP-1 and MMP-3 were not significantly different between SF-derived cells and ST-derived FLS (p = 0.20 and p = 0.40, respectively). There was no significant difference in the optical density value between two cell types in the cell invasion assay (p = 0.10). SF-derived adherent cells have a fibroblast-like phenotype from very early culture passages and have the potential to produce MMPs with the invasive capacity to degrade cartilage, identical to ST-derived FLS. Therefore, these cells could substitute for ST-derived FLS in studying the pathogenesis of RA.
Journal
Inflammation – Springer Journals
Published: Oct 11, 2008