Access the full text.
Sign up today, get DeepDyve free for 14 days.
T. Bozoǧlu, M. Özilgen, U. Bakir (1987)
Survival kinetics of lactic acid starter cultures during and after freeze dryingEnzyme and Microbial Technology, 9
A. Nussinovitch (1997)
Immobilization and encapsulation
B. Hall, A. McLoughlin, K. Leung, J. Trevors, Hung Lee (1998)
Transport and survival of alginate‐encapsulated and free lux‐lac marked Pseudomonas aeruginosa UG2Lr cells in soilFEMS Microbiology Ecology, 26
(1998)
Effects of Four Cryopro - tectants in Combination with Two Vehicle Solutions on Cultured Vascular Endothelial Cells
J. Fages (2004)
An optimized process for manufacturing anAzospirillum inoculant for cropsApplied Microbiology and Biotechnology, 32
Y. Bashan (1986)
Alginate Beads as Synthetic Inoculant Carriers for Slow Release of Bacteria That Affect Plant GrowthApplied and Environmental Microbiology, 51
(1992)
Use of Alginate and Other Carriers for Encapsulation of Microbial Cells for Use in Soil
D. Goldmeier, G. Scullard, M. Kapembwa, H. Lamba, G. Frize (2002)
Does increased aromatase activity in adipose fibroblasts cause low sexual desire in patients with HIV lipodystrophy?Sexually Transmitted Infections, 78
C. Champagne, N. Gardner, L. Soulignac, J. Innocent (2000)
The production of freeze‐dried immobilized cultures of Streptococcus thermophilus and their acidification properties in milkJournal of Applied Microbiology, 88
J. Fages (1992)
An Industrial View of Azospirillum Inoculants: Formulation and Application TechnologySymbiosis, 13
I. Katkov, N. Katkova, J. Critser, P. Mazur (1998)
Mouse spermatozoa in high concentrations of glycerol: chemical toxicity vs osmotic shock at normal and reduced oxygen concentrations.Cryobiology, 37 4
(1976)
Use of the scanning electron microscope for structural studies on soils and soil components
L. Chernin, A. Brandis, Z. Ismailov, I. Chet (1996)
Pyrrolnitrin Production by an Enterobacter agglomerans Strain with a Broad Spectrum of Antagonistic Activity Towards Fungal and Bacterial PhytopathogensCurrent Microbiology, 32
J. Elsas, A. Dijkstra, J. Govaert, J. Veen (1986)
Survival of Pseudomonas fluorescens and Bacillus subtilis introduced into two soils of different texture in field microplotsFems Microbiology Letters, 38
Y. Bashan (1998)
INOCULANTS OF PLANT GROWTH-PROMOTING BACTERIA FOR USE IN AGRICULTUREBiotechnology Advances, 16
C. Forni, E. Tel-or, E. Bar, M. Caiola (1991)
Effects of antibiotic treatments on Azolla-Anabaena and ArthrobacterPlant and Soil, 137
F. Barbirato, S. Astruc, P. Soucaille, Carole Camarasa, Jean Salmon, A. Bories (1997)
Anaerobic pathways of glycerol dissimilation by Enterobacter agglomerans CNCM 1210: limitations and regulations.Microbiology, 143 ( Pt 7)
T. Howdieshell, N. Bhalla, J. Dipiro, T. Kuske, R. Baisden (1995)
Effects of free glycerol contained in intravenous fat emulsion on plasma triglyceride determination.JPEN. Journal of parenteral and enteral nutrition, 19 2
C. Champagne, F. Mondou, Y. Raymond, E. Brochu (1996)
Effect of Immobilization in Alginate on the Stability of Freeze-Dried Bifidobacterium longumBioscience and microflora, 15
L. Chernin, Z. Ismailov, S. Haran, I. Chet (1995)
Chitinolytic Enterobacter agglomerans Antagonistic to Fungal Plant PathogensApplied and Environmental Microbiology, 61
L. Kearney, M. Upton, Aiden Loughlin (1990)
Enhancing the Viability of Lactobacillus plantarum Inoculum by Immobilizing the Cells in Calcium-Alginate Beads Incorporating CryoprotectantsApplied and Environmental Microbiology, 56
D. Rassis, I. Saguy, A. Nussinovitch (1998)
Physical Properties of Alginate−Starch Cellular SpongesJournal of Agricultural and Food Chemistry, 46
M. Ak, A. Nussinovitch, O. Campanella, M. Peleg (1989)
Crosslinking Rates of Thermally Preset Alginate GelsBiotechnology Progress, 5
J. Trevors, J. Elsas, Hung Lee, A. Wolters (1993)
Survival ofalginate-ecapsulated Pseudomonas fluorescens cells in soilApplied Microbiology and Biotechnology, 39
J. Elsas, J. Trevors, D. Jain, A. Wolters, C. Heijnen, L. Overbeek (1992)
Survival of, and root colonization by, alginate-encapsulated Pseudomonas fluorescens cells following introduction into soilBiology and Fertility of Soils, 14
I. Kim, Y. Baek, Y. Yoon (1996)
EFFECTS OF DEHYDRATION MEDIA AND IMMOBILIZATION IN CALCIUM-ALGINATE ON THE SURVIVAL OF LACTOBACILLUS CASEI AND BIFIDOBACTERIUM BIFIDUM, 18
C. Champagne, N. Gardner, E. Brochu, Y. Beaulieu (1991)
The Freeze-Drying of Lactic Acid Bacteria. A ReviewCanadian Institute of Food Science and Technology journal, 24
J. Whipps (1997)
Developments in the Biological Control of Soil-borne Plant PathogensAdvances in Botanical Research, 26
Y. Bashan, H. Levanony (1988)
Adsorption of the Rhizosphere Bacterium Azospirillum brasilense Cd to Soil, Sand and Peat ParticlesMicrobiology, 134
J. Elsas, L. Overbeek (1993)
Bacterial Responses to Soil Stimuli
Y. Tal, J. Rijn, A. Nussinovitch (1999)
Improvement of mechanical and biological properties of freeze-dried denitrifying alginate beads by using starch as a filler and carbon sourceApplied Microbiology and Biotechnology, 51
E. Paul, J. Fages, P. Blanc, G. Goma, A. Pareilleux (1993)
Survival of alginate-entrapped cells of Azospirillum lipoferum during dehydration and storage in relation to water propertiesApplied Microbiology and Biotechnology, 40
A. Nussinovitch, Z. Gershon (1997)
Alginate—oil spongesFood Hydrocolloids, 11
Improved viability of Gram‐negative bacteria during freeze‐dehydration, storage, and soil inoculation is of crucial importance to their efficient application. The chitinolytic Pantoae ( Enterobacter) agglomeransstrain IC1270, a potential biocontrol agent of soil‐borne plant‐pathogenic fungi, was used as a model organism to study the efficacy of freeze‐dried alginate‐based beads (macrocapsules) as possible carriers for immobilized Gram‐negative bacterial cells. These macrocapsules were produced by freeze‐dehydration of alginate gel spherical beads, in which different amounts of bacteria, glycerol, and colloidal chitin were entrapped. Subsequent drying produced different unexpected structures, pore‐size distributions, and changes in the outer and inner appearance of the resultant dried cellular solid. With increasing glycerol content, the proportion of larger pores increased. These structures can be related to changes in the slow‐release properties of the dried beads. The amount of glycerol in the beads differed from that in the alginate solution as a result of leakage during the beads' preparation and dehydration. Entrapping 109 cells per bead produced from alginate solution containing 30% glycerol and 1% chitin resulted in improved (in comparison to other studies) survival prospects (95%) during freeze‐drying. Moreover, immobilization of the bacterium sharply improved its survival in nonsterile irrigated and dry soils compared to bacteria in a water suspension. The results suggest that optimized conservation of Gram‐negative bacteria in dry glycerol‐containing alginate‐based cellular solids is not only possible but applicable for a variety of uses.
Biotechnology Progress – Wiley
Published: Jan 1, 2002
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.