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Preservation of Chitinolytic Pantoae agglomerans in a Viable Form by Cellular Dried Alginate‐Based Carriers

Preservation of Chitinolytic Pantoae agglomerans in a Viable Form by Cellular Dried... Improved viability of Gram‐negative bacteria during freeze‐dehydration, storage, and soil inoculation is of crucial importance to their efficient application. The chitinolytic Pantoae ( Enterobacter) agglomeransstrain IC1270, a potential biocontrol agent of soil‐borne plant‐pathogenic fungi, was used as a model organism to study the efficacy of freeze‐dried alginate‐based beads (macrocapsules) as possible carriers for immobilized Gram‐negative bacterial cells. These macrocapsules were produced by freeze‐dehydration of alginate gel spherical beads, in which different amounts of bacteria, glycerol, and colloidal chitin were entrapped. Subsequent drying produced different unexpected structures, pore‐size distributions, and changes in the outer and inner appearance of the resultant dried cellular solid. With increasing glycerol content, the proportion of larger pores increased. These structures can be related to changes in the slow‐release properties of the dried beads. The amount of glycerol in the beads differed from that in the alginate solution as a result of leakage during the beads' preparation and dehydration. Entrapping 109 cells per bead produced from alginate solution containing 30% glycerol and 1% chitin resulted in improved (in comparison to other studies) survival prospects (95%) during freeze‐drying. Moreover, immobilization of the bacterium sharply improved its survival in nonsterile irrigated and dry soils compared to bacteria in a water suspension. The results suggest that optimized conservation of Gram‐negative bacteria in dry glycerol‐containing alginate‐based cellular solids is not only possible but applicable for a variety of uses. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Progress Wiley

Preservation of Chitinolytic Pantoae agglomerans in a Viable Form by Cellular Dried Alginate‐Based Carriers

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References (33)

Publisher
Wiley
Copyright
Copyright © 2002 American Institute of Chemical Engineers (AIChE)
ISSN
8756-7938
eISSN
1520-6033
DOI
10.1021/bp025532t
pmid
12467443
Publisher site
See Article on Publisher Site

Abstract

Improved viability of Gram‐negative bacteria during freeze‐dehydration, storage, and soil inoculation is of crucial importance to their efficient application. The chitinolytic Pantoae ( Enterobacter) agglomeransstrain IC1270, a potential biocontrol agent of soil‐borne plant‐pathogenic fungi, was used as a model organism to study the efficacy of freeze‐dried alginate‐based beads (macrocapsules) as possible carriers for immobilized Gram‐negative bacterial cells. These macrocapsules were produced by freeze‐dehydration of alginate gel spherical beads, in which different amounts of bacteria, glycerol, and colloidal chitin were entrapped. Subsequent drying produced different unexpected structures, pore‐size distributions, and changes in the outer and inner appearance of the resultant dried cellular solid. With increasing glycerol content, the proportion of larger pores increased. These structures can be related to changes in the slow‐release properties of the dried beads. The amount of glycerol in the beads differed from that in the alginate solution as a result of leakage during the beads' preparation and dehydration. Entrapping 109 cells per bead produced from alginate solution containing 30% glycerol and 1% chitin resulted in improved (in comparison to other studies) survival prospects (95%) during freeze‐drying. Moreover, immobilization of the bacterium sharply improved its survival in nonsterile irrigated and dry soils compared to bacteria in a water suspension. The results suggest that optimized conservation of Gram‐negative bacteria in dry glycerol‐containing alginate‐based cellular solids is not only possible but applicable for a variety of uses.

Journal

Biotechnology ProgressWiley

Published: Jan 1, 2002

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