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Protein synthesis and growth in Octopus vulgaris

Protein synthesis and growth in Octopus vulgaris 227 106 106 2 2 D. F. Houlihan D. N. McMillan C. Agnisola I. Trara Genoino L. Foti Department of Zoology University of Aberdeen AB9 2TN Aberdeen Scotland Stazione Zoologica “Anton Dohrn” I-80121 Napoli Italy Department of General and Environmental Physiology University of Naples I-80134 Napoli Italy Abstract Rates of protein synthesis in the whole body and tissues of Octopus vulgaris collected in September 1985 and 1986 from the Bay of Naples were measured following a flooding dose injection of 3 H phenylalanine. There were stable phenylalanine free pool-specific radioactivities and linear incorporation of radiolabel into arm-tip protein from 10 to 30 min after the injection. In starved individuals there were no significant differences between the fractional rates of protein synthesis of the following tissues: ventricle, brain, branchial heart, arm tip, gill, stomach, arm, renal appendage and mantle. The mean value (± SE) for all the tissues was 3.02 ± 0.17% d −1 . In individuals fed varying amounts of crab, resulting in differing growth rates, there was a linear increase in fractional rates of whole-body protein synthesis with growth rate. A standard 148 g octopus growing at 3.0% d −1 synthesised 0.54 g of protein, with 0.43 g of this protein retained as growth. The proportion of the total protein synthesis which was retained as growth increased with increasing growth rate; at a maximum growth rate of 6% d −1 , over 90% of the protein synthesised was retained as growth. The ventricle, arm tip, gill, arm and mantle also showed similar patterns of a linear increase in fractional rates of protein synthesis with increased growth rates. The RNA concentrations in the whole body and tissues increased with increasing growth rates, but the major change was an increase in the efficiency of translation. It is concluded that rapid growth rates in O. vulgaris are brought about by high rates of protein synthesis and high efficiencies of retention of synthesised protein and, therefore, little protein degradation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Marine Biology Springer Journals

Protein synthesis and growth in Octopus vulgaris

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References (44)

Publisher
Springer Journals
Copyright
Copyright © 1990 by Springer-Verlag
Subject
Life Sciences; Biomedicine general; Oceanography; Ecology; Microbiology; Zoology
ISSN
0025-3162
eISSN
1432-1793
DOI
10.1007/BF01314808
Publisher site
See Article on Publisher Site

Abstract

227 106 106 2 2 D. F. Houlihan D. N. McMillan C. Agnisola I. Trara Genoino L. Foti Department of Zoology University of Aberdeen AB9 2TN Aberdeen Scotland Stazione Zoologica “Anton Dohrn” I-80121 Napoli Italy Department of General and Environmental Physiology University of Naples I-80134 Napoli Italy Abstract Rates of protein synthesis in the whole body and tissues of Octopus vulgaris collected in September 1985 and 1986 from the Bay of Naples were measured following a flooding dose injection of 3 H phenylalanine. There were stable phenylalanine free pool-specific radioactivities and linear incorporation of radiolabel into arm-tip protein from 10 to 30 min after the injection. In starved individuals there were no significant differences between the fractional rates of protein synthesis of the following tissues: ventricle, brain, branchial heart, arm tip, gill, stomach, arm, renal appendage and mantle. The mean value (± SE) for all the tissues was 3.02 ± 0.17% d −1 . In individuals fed varying amounts of crab, resulting in differing growth rates, there was a linear increase in fractional rates of whole-body protein synthesis with growth rate. A standard 148 g octopus growing at 3.0% d −1 synthesised 0.54 g of protein, with 0.43 g of this protein retained as growth. The proportion of the total protein synthesis which was retained as growth increased with increasing growth rate; at a maximum growth rate of 6% d −1 , over 90% of the protein synthesised was retained as growth. The ventricle, arm tip, gill, arm and mantle also showed similar patterns of a linear increase in fractional rates of protein synthesis with increased growth rates. The RNA concentrations in the whole body and tissues increased with increasing growth rates, but the major change was an increase in the efficiency of translation. It is concluded that rapid growth rates in O. vulgaris are brought about by high rates of protein synthesis and high efficiencies of retention of synthesised protein and, therefore, little protein degradation.

Journal

Marine Biology – Springer Journals

Published: Jun 1, 1990

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