Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Endocannabinoid transport tightly controls 2‐arachidonoyl glycerol actions in the hippocampus: effects of low temperature and the transport inhibitor AM404

Endocannabinoid transport tightly controls 2‐arachidonoyl glycerol actions in the hippocampus:... The control of endocannabinoid actions on cortical neurons by a putative carrier‐mediated uptake is still poorly understood at the level of synaptic transmission. We investigated the effect of an endocannabinoid, 2‐arachidonoyl glycerol (2‐AG), on inhibitory postsynaptic currents (IPSCs) in hippocampal slices under physiological conditions, and when uptake was altered by a selective blocker or lower temperature. Bath application of 2‐AG (20 µm) caused a 40% reduction in the amplitude of IPSCs evoked in the perisomatic region of CA1 pyramidal neurons at room temperature; this effect could be blocked by a specific CB1 receptor antagonist, AM251. By contrast, a smaller (20%) but significant suppression of inhibitory transmission was found by 2‐AG at 33–35 °C. This reduced blocking effect at physiological temperature could be brought back to 40% by coapplying the endocannabinoid uptake blocker, AM404 (10 or 20 µm) with 2‐AG. In parallel experiments, we measured (3H)2‐AG uptake at different temperatures in primary cultures prepared from cortical neurons. These data confirmed a striking inhibition of (3H)2‐AG uptake at room temperature compared with values observed at 37 °C. Uptake could be significantly modified by anandamide, 2‐AG and AM404, suggesting a common transporter for the two endocannabinoids. These findings together demonstrate the presence of an effective endocannabinoid uptake in cortical neurons, which could considerably alter the spatial and temporal constraints of endocannabinoid signalling at physiological temperature, and which may critically change the interpretation of findings at room temperature. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Neuroscience Wiley

Endocannabinoid transport tightly controls 2‐arachidonoyl glycerol actions in the hippocampus: effects of low temperature and the transport inhibitor AM404

Download PDF
6 pages

Loading next page...
 
/lp/wiley/endocannabinoid-transport-tightly-controls-2-arachidonoyl-glycerol-YMkcqGP9Ie

References (26)

Publisher
Wiley
Copyright
Copyright © 2004 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0953-816X
eISSN
1460-9568
DOI
10.1111/j.0953-816X.2004.03433.x
pmid
15182306
Publisher site
See Article on Publisher Site

Abstract

The control of endocannabinoid actions on cortical neurons by a putative carrier‐mediated uptake is still poorly understood at the level of synaptic transmission. We investigated the effect of an endocannabinoid, 2‐arachidonoyl glycerol (2‐AG), on inhibitory postsynaptic currents (IPSCs) in hippocampal slices under physiological conditions, and when uptake was altered by a selective blocker or lower temperature. Bath application of 2‐AG (20 µm) caused a 40% reduction in the amplitude of IPSCs evoked in the perisomatic region of CA1 pyramidal neurons at room temperature; this effect could be blocked by a specific CB1 receptor antagonist, AM251. By contrast, a smaller (20%) but significant suppression of inhibitory transmission was found by 2‐AG at 33–35 °C. This reduced blocking effect at physiological temperature could be brought back to 40% by coapplying the endocannabinoid uptake blocker, AM404 (10 or 20 µm) with 2‐AG. In parallel experiments, we measured (3H)2‐AG uptake at different temperatures in primary cultures prepared from cortical neurons. These data confirmed a striking inhibition of (3H)2‐AG uptake at room temperature compared with values observed at 37 °C. Uptake could be significantly modified by anandamide, 2‐AG and AM404, suggesting a common transporter for the two endocannabinoids. These findings together demonstrate the presence of an effective endocannabinoid uptake in cortical neurons, which could considerably alter the spatial and temporal constraints of endocannabinoid signalling at physiological temperature, and which may critically change the interpretation of findings at room temperature.

Journal

European Journal of Neuroscience – Wiley

Published: Jun 1, 2004

There are no references for this article.