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Developmental regulation of Islet‐1 mRNA expression during neuronal differentiation in embryonic zebrafish

Developmental regulation of Islet‐1 mRNA expression during neuronal differentiation in embryonic... Islet‐1 (Isl‐1) is a LIM domain/homeodomain‐type transcription regulator that has been originally identified as an insulin gene enhancer binding protein. Isl‐1 is also expressed by subsets of neurons in the central nervous system of rat and chick embryos. We have cloned the Isl‐1 cDNA from zebrafish and examined its expression pattern using in situ hybridization to whole‐mount embryos. Isl‐1 mRNA first appears immediately after gastrulation in the polster, the cranial ganglia, and in Rohon‐Beard neurons and ventromedial cells of the spinal cord. The expression by the ventromedial cells is segmentally repeated and becomes restricted to the one or two cells slightly anterior to the segment borders. Double staining by in situ hybridization and an antibody which stains most axons suggested that these segmentally distributed cells may be either the rostral primary motoneuron (RoP) or middle primary motoneuron (MiP). This raises a possibility that Isl‐1 may be involved during determination of subtype identities of the primary motoneurons. Furthermore, the specific Isl‐1 mRNA expression in the spinal cord is under the control of the somites, since mutant embryo with defective somite failed to maintain this pattern. © 1994 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Developmental Dynamics Wiley

Developmental regulation of Islet‐1 mRNA expression during neuronal differentiation in embryonic zebrafish

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References (41)

Publisher
Wiley
Copyright
Copyright © 1994 Wiley‐Liss, Inc.
ISSN
1058-8388
eISSN
1097-0177
DOI
10.1002/aja.1001990102
pmid
8167375
Publisher site
See Article on Publisher Site

Abstract

Islet‐1 (Isl‐1) is a LIM domain/homeodomain‐type transcription regulator that has been originally identified as an insulin gene enhancer binding protein. Isl‐1 is also expressed by subsets of neurons in the central nervous system of rat and chick embryos. We have cloned the Isl‐1 cDNA from zebrafish and examined its expression pattern using in situ hybridization to whole‐mount embryos. Isl‐1 mRNA first appears immediately after gastrulation in the polster, the cranial ganglia, and in Rohon‐Beard neurons and ventromedial cells of the spinal cord. The expression by the ventromedial cells is segmentally repeated and becomes restricted to the one or two cells slightly anterior to the segment borders. Double staining by in situ hybridization and an antibody which stains most axons suggested that these segmentally distributed cells may be either the rostral primary motoneuron (RoP) or middle primary motoneuron (MiP). This raises a possibility that Isl‐1 may be involved during determination of subtype identities of the primary motoneurons. Furthermore, the specific Isl‐1 mRNA expression in the spinal cord is under the control of the somites, since mutant embryo with defective somite failed to maintain this pattern. © 1994 Wiley‐Liss, Inc.

Journal

Developmental Dynamics – Wiley

Published: Jan 1, 1994

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