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J. Vane, R. Gryglewski, R. Botting (1987)
The endothelial cell as a metabolic and endocrine organTrends in Pharmacological Sciences, 8
R. Rapoport, S. Waldman, K. Schwartz, R. Winquist, F. Murad (1985)
Effects of atrial natriuretic factor, sodium nitroprusside, and acetylcholine on cyclic GMP levels and relaxation in rat aorta.European journal of pharmacology, 115 2-3
N. Robinson, J. Dabney, W. Weidner, G. Jones, J. Scott (1975)
Vascular responses in the equine digit.American journal of veterinary research, 36 08
Bryant (1992)
Characterisation of alpha-adrenoceptors in the hind limb venous system of the horseBr. J. Pharmacol., 107
C. Kennedy, G. Burnstock (1985)
Evidence for two types of P2-purinoceptor in longitudinal muscle of the rabbit portal vein.European journal of pharmacology, 111 1
G. Rubanyi, J. Romero, P. Vanhoutte (1986)
Flow-induced release of endothelium-derived relaxing factor.The American journal of physiology, 250 6 Pt 2
R. Palmer, D. Ashton, S. Moncada (1988)
Vascular endothelial cells synthesize nitric oxide from L-arginineNature, 333
D. Allen, R. Korthuis, S. Clark (1988)
Evaluation of Starling forces in the equine digit.Journal of applied physiology, 64 4
J. Gordon, W. Martin (1983)
Stimulation of endothelial prostacyclin production plays no role in endothelium‐dependent relaxation of the pig aortaBritish Journal of Pharmacology, 80
Rubanyi (1988)
Heterogeneity of endothelium-dependent responses to acetylcholine in canine femoral arteries and veinsBlood vessels, 25
G. Rubanyi, P. Vanhoutte (1988)
Heterogeneity of endothelium-dependent responses to acetylcholine in canine femoral arteries and veins. Separation of the role played by endothelial and smooth muscle cells.Blood vessels, 25 2
Elliott (1993)
Characterisation of the responses of equine digital veins to adenosine and its analoguesBr. J. Pharmacol., 110
J. Poole, A. Sanders, H. Florey (1958)
The regeneration of aortic endothelium.The Journal of pathology and bacteriology, 75 1
T. Cocks, J. Angus (1983)
Endothelium-dependent relaxation of coronary arteries by noradrenaline and serotoninNature, 305
Angus (1986)
The a adrenoceptor on endothelial cellsFederation Proc., 45
M. Golub, M. Berger, M. Powell (1985)
Adrenergic stimulation of prostacyclin production in the rat tail artery. I. Response to agonists and antagonists.Prostaglandins, leukotrienes, and medicine, 20 3
P. Vanhoutte, V. Miller (1985)
Heterogeneity of Endothelium‐Dependent Responses in Mammalian Blood VesselsJournal of Cardiovascular Pharmacology, 7
W. Martin, N. Cusack, J. Carleton, J. Gordon (1985)
Specificity of P2-purinoceptor that mediates endothelium-dependent relaxation of the pig aorta.European journal of pharmacology, 108 3
R. Rapoport, F. Murad (1983)
Agonist‐Induced Endothelium‐Dependent Relaxation in Rat Thoracic Aorta May Be Mediated through cGMPCirculation Research, 52
L. Ignarro, R. Byrns, G. Buga, K. Wood (1987)
Endothelium‐Derived Relaxing Factor From Pulmonary Artery and Vein Possesses Pharmacologic and Chemical Properties Identical to Those of Nitric Oxide RadicalCirculation Research, 61
W. Martin, R. Furchgott, G. Villani, D. Jothianandan (1986)
Depression of contractile responses in rat aorta by spontaneously released endothelium-derived relaxing factor.The Journal of pharmacology and experimental therapeutics, 237 2
Zhihong Yang, Ludwig, von, Segesser, Erwin Bauer, P. Stulz, M. Turina, Thomas Luischer (1991)
Different activation of the endothelial L-arginine and cyclooxygenase pathway in the human internal mammary artery and saphenous vein.Circulation research, 68 1
I. Sakuma, D. Stuehrt, S. Gross, Carl NATHANt, R. Levi (1988)
Identification of arginine as a precursor of endothelium-derived relaxing factor.Proceedings of the National Academy of Sciences of the United States of America, 85 22
Hood (1978)
Equine laminitis I: Radioisotopic analysis of the hemodynamics of the foot during the acute diseaseJ. equine Med. Surg., 2
G. Burnstock, C. Kennedy (1986)
A Dual Function for Adenosine 5′‐Triphosphate in the Regulation of Vascular Tone: Excitatory Cotransmitter with Noradrenaline from Perivascular Nerves and Locally Released Inhibitory Intravascular AgentCirculation Research, 58
V. Ralevic, G. Burnstock (1988)
Actions mediated by P2‐purinoceptorsubtypes in the isolated perfused mesenteric bed of the ratBritish Journal of Pharmacology, 95
R. Palmer, A. Ferrige, S. Moncada (1987)
Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factorNature, 327
Vallance (1989)
Nitric oxide synthesised from L-arginine mediates endothelium-dependent dilation in human veinsCardiovasc. Res., 23
S. Moncada, R. Palmer, E. Higgs (1991)
Nitric oxide: physiology, pathophysiology, and pharmacology.Pharmacological reviews, 43 2
G. Baxter, R. Laskey, R. Tackett, J. Moore, D. Allen (1989)
In vitro reactivity of digital arteries and veins to vasoconstrictive mediators in healthy horses and in horses with early laminitis.American journal of veterinary research, 50 4
J. Mey, P. Vanhoutte (1982)
Heterogeneous Behavior of the Canine Arterial and Venous Wall: Importance of the EndotheliumCirculation Research, 51
D. Rees, R. Palmer, S. Moncada (1989)
Role of endothelium-derived nitric oxide in the regulation of blood pressure.Proceedings of the National Academy of Sciences of the United States of America, 86 9
Rubanyi Gm, Vanhoutte Pm (1988)
Heterogeneity of endothelium-dependent responses to acetylcholine in canine femoral arteries and veins. Separation of the role played by endothelial and smooth muscle cells., 25
J. McGrath, S. Monaghan, A. Templeton, V. Wilson (1990)
Effects of basal and acetylcholine‐induced release of endothelium‐derived relaxing factor on contraction to α‐adrenoceptor agonists in a rabbit artery and corresponding veinsBritish Journal of Pharmacology, 99
G. Nucci, Richard Gryglewski, Timothy Warner, John Vane (1988)
Receptor-mediated release of endothelium-derived relaxing factor and prostacyclin from bovine aortic endothelial cells is coupled.Proceedings of the National Academy of Sciences of the United States of America, 85 7
R. Gryglewski, R. Palmer, S. Moncada (1986)
Superoxide anion is involved in the breakdown of endothelium-derived vascular relaxing factorNature, 320
U. Pohl, J. Holtz, R. Busse, E. Bassenge (1986)
Crucial role of endothelium in the vasodilator response to increased flow in vivo.Hypertension, 8 1
R. Furchgott, J. Zawadzki (1980)
The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholineNature, 288
Hunt (1991)
The pathophysiology of acute laminitisComp. Cont. pract. vet. Ed., 13
D. Rees, R. Palmer, R. Schulz, H. Hodson, S. Moncada (1990)
Characterization of three inhibitors of endothelial nitric oxide synthase in vitro and in vivoBritish Journal of Pharmacology, 101
I. Fridovich, P. Hagen, J. Murray (1987)
Endothelium-Derived Relaxing Factor: In Search of the Endogenous NitroglycerinPhysiology, 2
G. Mann, J. Pearson, C. Sheriff, V. Toothill (1989)
Expression of amino acid transport systems in cultured human umbilical vein endothelial cells.The Journal of Physiology, 410
R. Miller, M. Mony, V. Schini, P. Schoeffter, J. Stoclet (1984)
Endothelial mediated inhibition of contraction and increase in cyclic GMP levels evoked by the α‐adrenoceptor agonist B‐HT 920 in rat isolated aortaBritish Journal of Pharmacology, 83
G. Martin, M. Bolofo, Heather Giles (1992)
Inhibition of endothelium‐dependent vasorelaxation by arginine analogues: a pharmacological analysis of agonist and tissue dependenceBritish Journal of Pharmacology, 105
M. Randall, Alexander Kay, C. Hiley (1988)
Endothelium‐dependent modulation of the pressor activity of arginine vasopressin in the isolated superior mesenteric arterial bed of the ratBritish Journal of Pharmacology, 95
Summary Isolated equine digital veins were examined in vitro to study the importance of the endothelium in the responses to both vasodilator and vasoconstrictor agents and to characterise the endothelial‐derived mediators involved. Carbachol (Cch; 1 μM) and bradykinin (Bk; 1 nM) caused relaxation of U44069‐induced tone by 79.5 ± 0.35 % and 73.7 ± 4.0 % respectively. Mechanical removal of the endothelium completely prevented relaxant responses to Cch and to Bk showing they were mediated by the endothelium. Treatment of veins with NG‐nitro‐L‐arginine methyl ester (L‐NAME; 30 and 300 μM) inhibited vasorelaxant responses to both Cch and Bk whereas the cyclooxygenase inhibitor, ibuprofen (10 μM) had no inhibitory effect. The inhibitory action of L‐NAME on the relaxations produced by Cch was partly reversed by L‐arginine (3 and 10 mM). Cch‐relaxations were potentiated in the presence of super oxide dismutase (15 units/ml) and inhibited by methylene blue (10 μM). The vasorelaxant effects of ATP (0.01 μM to 0.1 μM) were not dependent on the presence of the endothelium and the selective P2y receptor agonist, 2‐methylthio‐ATP proved to be ineffective as a vasodilator. Removal of the endothelium did not enhance the vasoconstrictor effects of the α1 adrenoceptor agonist phenylephrine (0.01 μM to 0.1 μM) and treatment with L‐NAME (300 μM) did not change the vasoconstrictor responses to 5‐HT (1 nM to 10 μM) or the α2 adrenoceptor agonist BHT‐920 (1 nM to 1 μM). These data suggest the L‐arginine nitric oxide pathway is of primary importance in mediating endothelium dependent relaxations to Cch and BK in equine digital veins but that basal release of nitric oxide is insufficient, under the conditions used in the present study, to modulate responses to vasoconstrictor agents. No functional evidence has been found for P2y‐receptors, α2‐adrenoceptors or 5‐HT receptors on endothelial cells of equine digital veins linked to nitric oxide production.
Equine Veterinary Journal – Wiley
Published: Sep 1, 1994
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