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Bone marrow interface: Preferential attachment of an osteoblastic marrow stromal cell line

Bone marrow interface: Preferential attachment of an osteoblastic marrow stromal cell line In this study, we report on the cell adhesion properties of marrow stromal cells to extracellular matrix components such as collagen and noncollagenous proteins. The osteoblastic cells and their non‐osteoblastic counter‐parts (MBA series) from the marrow stroma differentially recognized a spectrum of extracellular matrix proteins. The osteoblastic cells, MBA‐15, preferentially attached to bone matrix proteins, whereas fibroendothelial MBA‐2.1 and adipocytic 14F1.1 cells did not. The MBA‐15 cells demonstrated a preference in their attachment to fibronectin > mixture of collagens > bone matrix extracts > collagen type 1 > noncollagenous proteins. Clonal subpopulations derived from the MBA‐15 cell line representing various stages along the osteogenic lineage expressed differential attachment preference. MBA‐15.4, a less differentiated clonal line, was compared to MBA‐15.6, a mature cell line. © 1995 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Cellular Biochemistry Wiley

Bone marrow interface: Preferential attachment of an osteoblastic marrow stromal cell line

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References (55)

Publisher
Wiley
Copyright
Copyright © 1995 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0730-2312
eISSN
1097-4644
DOI
10.1002/jcb.240590204
pmid
8904309
Publisher site
See Article on Publisher Site

Abstract

In this study, we report on the cell adhesion properties of marrow stromal cells to extracellular matrix components such as collagen and noncollagenous proteins. The osteoblastic cells and their non‐osteoblastic counter‐parts (MBA series) from the marrow stroma differentially recognized a spectrum of extracellular matrix proteins. The osteoblastic cells, MBA‐15, preferentially attached to bone matrix proteins, whereas fibroendothelial MBA‐2.1 and adipocytic 14F1.1 cells did not. The MBA‐15 cells demonstrated a preference in their attachment to fibronectin > mixture of collagens > bone matrix extracts > collagen type 1 > noncollagenous proteins. Clonal subpopulations derived from the MBA‐15 cell line representing various stages along the osteogenic lineage expressed differential attachment preference. MBA‐15.4, a less differentiated clonal line, was compared to MBA‐15.6, a mature cell line. © 1995 Wiley‐Liss, Inc.

Journal

Journal of Cellular BiochemistryWiley

Published: Oct 1, 1995

Keywords: ; ; ; ;

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