Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/wiley/the-dead-box-protein-p68-a-novel-transcriptional-coactivator-of-the-XcjA86SVy0
References (44)
-
K. Rossow, R. Janknecht (2003)
Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300Oncogene, 22
-
R. Iggo, D. Lane (1989)
Nuclear protein p68 is an RNA‐dependent ATPase.The EMBO Journal, 8
-
V. Ogilvie, Brian Wilson, S. Nicol, N. Morrice, Laura Saunders, Glen Barber, F. Fuller-Pace (2003)
The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells.Nucleic acids research, 31 5
-
Suzanne Szak, D. Mays, J. Pietenpol (2001)
Kinetics of p53 Binding to Promoter Sites In VivoMolecular and Cellular Biology, 21
-
C. Venot, M. Maratrat, V. Sierra, E. Conseiller, L. Debussche (1999)
Definition of a p53 transactivation function-deficient mutant and characterization of two independent p53 transactivation subdomainsOncogene, 18
-
Manuel Rodríguez, Jill Thompson, R. Hay, C. Dargemont (1999)
Nuclear Retention of IκBα Protects It from Signal-induced Degradation and Inhibits Nuclear Factor κB Transcriptional Activation*The Journal of Biological Chemistry, 274
-
Andrew Bond, D. Mangus, F. He, A. Jacobson (2001)
Absence of Dbp2p Alters Both Nonsense-Mediated mRNA Decay and rRNA ProcessingMolecular and Cellular Biology, 21
-
H. Uhlmann-Schiffler, O. Rössler, H. Stahl (2002)
The mRNA of DEAD Box Protein p72 Is Alternatively Translated into an 82-kDa RNA Helicase*The Journal of Biological Chemistry, 277
-
S. Guil, R. Gattoni, M. Carrascal, J. Abian, J. Stévenin, M. Bach-Elias (2003)
Roles of hnRNP A1, SR Proteins, and p68 Helicase in c-H-ras Alternative Splicing RegulationMolecular and Cellular Biology, 23
-
Harald Hirling, Martin Scheffner, Tobias Restle, Hans Stahl (1989)
RNA helicase activity associated with the human p68 proteinNature, 339
-
C. Midgley, J. Desterro, M. Saville, Stephanie Howard, A. Sparks, R. Hay, D. Lane (2000)
An N-terminal p14ARF peptide blocks Mdm2-dependent ubiquitination in vitro and can activate p53 in vivoOncogene, 19
-
N. Tanner, P. Linder (2001)
DExD/H box RNA helicases: from generic motors to specific dissociation functions.Molecular cell, 8 2
-
H. Endoh, K. Maruyama, Y. Masuhiro, Yoko Kobayashi, M. Goto, H. Tai, J. Yanagisawa, D. Metzger, S. Hashimoto, S. Kato (1999)
Purification and Identification of p68 RNA Helicase Acting as a Transcriptional Coactivator Specific for the Activation Function 1 of Human Estrogen Receptor αMolecular and Cellular Biology, 19
-
Yili Yin, Charles Stephen, M. Luciani, R. Fåhraeus (2002)
p53 stability and activity is regulated by Mdm2-mediated induction of alternative p53 translation productsNature Cell Biology, 4
-
M. Watanabe, J. Yanagisawa, H. Kitagawa, K. Takeyama, S. Ogawa, Y. Arao, M. Suzawa, Y. Kobayashi, T. Yano, H. Yoshikawa, Y. Masuhiro, S. Kato (2001)
A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor alpha coactivator through the N-terminal activation domain (AF-1) with an RNA coactivator, SRA.The EMBO journal, 20 6
-
G. Webster, N. Perkins (1999)
Transcriptional Cross Talk between NF-κB and p53Molecular and Cellular Biology, 19
-
G. Webster, N. Perkins (1999)
Transcriptional cross talk between NF-kappaB and p53.Molecular and cellular biology, 19 5
-
R. Iggo, D. Jamieson, S. MacNeill, J. Southgate, J. McPheat, D. Lane (1991)
p68 RNA helicase: identification of a nucleolar form and cloning of related genes containing a conserved intron in yeastsMolecular and Cellular Biology, 11
-
S. Courtois, G. Verhaegh, S. North, M. Luciani, P. Lassus, U. Hibner, M. Oren, P. Hainaut (2002)
ΔN-p53, a natural isoform of p53 lacking the first transactivation domain, counteracts growth suppression by wild-type p53Oncogene, 21
-
Gâbor Lamm, S. Nicol, F. Fuller-Pace, A. Lamond (1996)
p72: a human nuclear DEAD box protein highly related to p68.Nucleic acids research, 24 19
-
M. Ford, I. Anton, D. Lane (1988)
Nuclear protein with sequence homology to translation initiation factor eIF-4ANature, 332
-
T. Kodadek (2004)
Faculty Opinions recommendation of Estrogen receptor-alpha directs ordered, cyclical, and combinatorial recruitment of cofactors on a natural target promoter. -
E. Balint, K. Vousden (2001)
Activation and activities of the p53 tumour suppressor proteinBritish Journal of Cancer, 85
-
E. Miyashita, Bin Yang, K. Lam, D. Crawford, D. Thorley-Lawson (1995)
A novel form of Epstein-Barr virus latency in normal B cells in vivoCell, 80
-
R. Métivier, G. Penot, Michaela Hübner, G. Reid, H. Brand, M. Koš, F. Gannon (2003)
Estrogen Receptor-α Directs Ordered, Cyclical, and Combinatorial Recruitment of Cofactors on a Natural Target PromoterCell, 115
-
Miyashita Toshiyuki, John Reed (1995)
Tumor suppressor p53 is a direct transcriptional activator of the human bax geneCell, 80
-
J. Westermarck, C. Weiss, R. Saffrich, J. Kast, A. Musti, Matthias Wessely, W. Ansorge, B. Séraphin, M. Wilm, B. Valdez, D. Bohmann (2002)
The DEXD/H‐box RNA helicase RHII/Gu is a co‐factor for c‐Jun‐activated transcriptionThe EMBO Journal, 21
-
Mirsada Causevic, Mirsada Causevic, R. Hislop, N. Kernohan, F. Carey, R. Kay, R. Steele, F. Fuller-Pace (2001)
Overexpression and poly-ubiquitylation of the DEAD-box RNA helicase p68 in colorectal tumoursOncogene, 20
-
B. Vogelstein, D. Lane, A. Levine (2000)
Surfing the p53 networkNature, 408
-
S. Courtois, G. Verhaegh, S. North, M. Luciani, P. Lassus, U. Hibner, M. Oren, P. Hainaut (2002)
DeltaN-p53, a natural isoform of p53 lacking the first transactivation domain, counteracts growth suppression by wild-type p53.Oncogene, 21 44
-
Michiko Watanabe, J. Yanagisawa, H. Kitagawa, K. Takeyama, S. Ogawa, Y. Arao, M. Suzawa, Yoko Kobayashi, T. Yano, H. Yoshikawa, Y. Masuhiro, S. Kato (2001)
Retracted: A subfamily of RNA‐binding DEAD‐box proteins acts as an estrogen receptor α coactivator through the N‐terminal activation domain (AF‐1) with an RNA coactivator, SRAThe EMBO Journal, 20
-
W. El-Deiry, T. Tokino, V. Velculescu, D. Levy, R. Parsons, J. Trent, D. Lin, W. Mercer, K. Kinzler, B. Vogelstein (1993)
WAF1, a potential mediator of p53 tumor suppressionCell, 75
-
H. Endoh, K. Maruyama, Y. Masuhiro, Y. Kobayashi, M. Goto, H. Tai, J. Yanagisawa, D. Metzger, S. Hashimoto, S. Kato (1999)
Purification and identification of p68 RNA helicase acting as a transcriptional coactivator specific for the activation function 1 of human estrogen receptor alpha.Molecular and cellular biology, 19 8
-
Valérie Deguin-Chambon, M. Vacher, Martial Jullien, E. May, J. Bourdon (2000)
Direct transactivation of c-Ha-Ras gene by p53: evidence for its involvement in p53 transactivation activity and p53-mediated apoptosisOncogene, 19
-
Isabelle lost, M. Dreyfus (1994)
mRNAs can be stabilized by DEAD-box proteinsNature, 372
-
J. Espinosa, R. Verdun, B. Emerson (2003)
p53 functions through stress- and promoter-specific recruitment of transcription initiation components before and after DNA damage.Molecular cell, 12 4
-
E. Harlow, L. Crawford, D. Pim, N. Williamson (1981)
Monoclonal antibodies specific for simian virus 40 tumor antigensJournal of Virology, 39
-
Zhi-Ren Liu (2002)
p68 RNA Helicase Is an Essential Human Splicing Factor That Acts at the U1 snRNA-5′ Splice Site DuplexMolecular and Cellular Biology, 22
-
T. Nakajima, C. Uchida, S. Anderson, Chee-gun Lee, J. Hurwitz, J. Parvin, M. Montminy (1997)
RNA Helicase A Mediates Association of CBP with RNA Polymerase IICell, 90
-
M. Rodriguez, J. Thompson, R. Hay, C. Dargemont (1999)
Nuclear retention of IkappaBalpha protects it from signal-induced degradation and inhibits nuclear factor kappaB transcriptional activation.The Journal of biological chemistry, 274 13
-
J. Dignam, R. Lebovitz, R. Roeder (1983)
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.Nucleic acids research, 11 5
-
Rebecca Stevenson, Stuart Hamilton, D. Maccallum, P. Hall, F. Fuller-Pace (1998)
Expression of the ‘DEAD box’ RNA helicase p68 is developmentally and growth regulated and correlates with organ differentiation/maturation in the fetusThe Journal of Pathology, 184
-
Appropriate anti-mouse, anti-rabbit and anti-goat secondary antibodies were obtained from DAKO -
J. Hsieh, Florence Chan, D. O’Connor, Sibylle Mittnacht, Shan Zhong, Xin Lu (1999)
RB regulates the stability and the apoptotic function of p53 via MDM2.Molecular cell, 3 2
- Publisher
- Wiley
- Copyright
- Copyright © 2013 Wiley Periodicals, Inc
- ISSN
- 0261-4189
- eISSN
- 1460-2075
- DOI
- 10.1038/sj.emboj.7600550
- pmid
- 15660129
- Publisher site
- See Article on Publisher Site
Abstract
The DEAD box RNA helicase, p68, has been implicated in various cellular processes and has been shown to possess transcriptional coactivator function. Here, we show that p68 potently synergises with the p53 tumour suppressor protein to stimulate transcription from p53‐dependent promoters and that endogenous p68 and p53 co‐immunoprecipitate from nuclear extracts. Strikingly, RNAi suppression of p68 inhibits p53 target gene expression in response to DNA damage, as well as p53‐dependent apoptosis, but does not influence p53 stabilisation or expression of non‐p53‐responsive genes. We also show, by chromatin immunoprecipitation, that p68 is recruited to the p21 promoter in a p53‐dependent manner, consistent with a role in promoting transcriptional initiation. Interestingly, p68 knock‐down does not significantly affect NF‐κB activation, suggesting that the stimulation of p53 transcriptional activity is not due to a general transcription effect. This study represents the first report of the involvement of an RNA helicase in the p53 response, and highlights a novel mechanism by which p68 may act as a tumour cosuppressor in governing p53 transcriptional activity.
Journal
The EMBO Journal – Wiley
Published: Sep 9, 2005
Keywords: ; ; ; ;