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Kevin Powell, A. Gatehouse, V. Hilder, E. Damme, W. Peumans, J. Boonjawat, K. Horsham, J. Gatehouse (1995)
Different antimetabolic effects of related lectins towards nymphal stages of Nilaparvata lugensEntomologia Experimentalis et Applicata, 75
J. Balzarini, D. Schols, Johan, Neyts, Els Damme, W. Peumans, '. Erikdeclercq (1991)
Alpha-(1-3)- and alpha-(1-6)-D-mannose-specific plant lectins are markedly inhibitory to human immunodeficiency virus and cytomegalovirus infections in vitroAntimicrobial Agents and Chemotherapy, 35
G. Hester, H. Kaku, I. Goldstein, C. Wright (1995)
Structure of mannose-specific snowdrop (Galanthus nivalis) lectin is representative of a new plant lectin familyNature Structural Biology, 2
(1995)
The mannose binding monocot lectins and their genes
E. Damme, W. Peumans (1993)
Cell-Free Synthesis of Lectins
E. Damme, K. Smeets, Iris Engelborghs, Helen Aelbers, J. Balzarini, A. Pusztai, F. Leuven, I. Goldstein, W. Peumans (1993)
Cloning and characterization of the lectin cDNA clones from onion, shallot and leekPlant Molecular Biology, 23
E. Damme, K. Smeets, S. Torrekens, F. Leuven, I. Goldstein, W. Peumans (1992)
The closely related homomeric and heterodimeric mannose-binding lectins from garlic are encoded by one-domain and two-domain lectin genes, respectively.European journal of biochemistry, 206 2
M. Kozak (1981)
Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.Nucleic acids research, 9 20
V. Hilder, Kevin Powell, A. Gatehouse, J. Gatehouse, L. Gatehouse, Y. Shi, William Hamilton, A. Merryweather, Christine Newell, J. Timans, Willy Peumans, E. Damme, Donald Boulter (2005)
Expression of snowdrop lectin in transgenic tobacco plants results in added protection against aphidsTransgenic Research, 4
R. Mierendorf, D. Pfeffer (1987)
Direct sequencing of denatured plasmid DNA.Methods in enzymology, 152
Y. Rahbé, N. Sauvion, G. Febvay, W. Peumans, A. Gatehouse (1995)
Toxicity of lectins and processing of ingested proteins in the pea aphid Acyrthosiphon pisumEntomologia Experimentalis et Applicata, 76
R. Mierendorf, D. Pfeffer (1987)
[58] Direct sequencing of denatured plasmid DNAMethods in Enzymology, 152
J. Damme, K. Smeets, S. Torrekens, F. Leuven, W. Peumans (1993)
The mannose-specific lectins from ramsons (Allium ursinum L.) are encoded by three sets of genes.European journal of biochemistry, 217 1
L. Lemesle-Varloot, B. Henrissat, C. Gaboriaud, C. Gaboriaud, V. Bissery, A. Morgat, A. Morgat, J. Mornon (1990)
Hydrophobic cluster analysis: procedures to derive structural and functional information from 2-D-representation of protein sequences.Biochimie, 72 8
C. Gaboriaud, V. Bissery, T. Benchetrit, J. Mornon (1987)
Hydrophobic cluster analysis: An efficient new way to compare and analyse amino acid sequencesFEBS Letters, 224
G. Hester, H. Kaku, I. Goldstein, C. Wright (1995)
Erratum: Structure of mannose-specific snowdrop (Galanthus nivalis) lectin is representative of a new plant lectin familyNature Structural Biology, 2
E. Damme, H. Kaku, F. Perini, I. Goldstein, B. Peeters, F. Yagi, B. Decock, W. Peumans (1991)
Biosynthesis, primary structure and molecular cloning of snowdrop (Galanthus nivalis L.) lectin.European journal of biochemistry, 202 1
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
F. Sanger, S. Nicklen, A. Coulson (1977)
DNA sequencing with chain-terminating inhibitors.Proceedings of the National Academy of Sciences of the United States of America, 74 12
M. Dubois, K. Gilles, J. Hamilton, P. Rebers, F. Smith (1956)
Colorimetric Method for Determination of Sugars and Related SubstancesAnalytical Chemistry, 28
G. Heijne (1986)
A new method for predicting signal sequence cleavage sites.Nucleic Acids Research, 14
Two novel lectins were isolated from roots and leaves of garlic. Characterization of the purified proteins indicated that the leaf lectin ASAL is a dimer of two identical subunits of 12 kDa, which closely resembles the leaf lectins from onion, leek and shallot with respect to its molecular structure and agglutination activity. In contrast, the root lectin ASARI, which is a dimer of subunits of 15 kDa, strongly differs from the leaf lectin with respect to its agglutination activity. cDNA cloning of the leaf and root lectins revealed that the deduced amino acid sequences of ASAL and ASARI are virtually identical. Since both lectins have identical N-terminal sequences the larger Mr of the ASARI subunits implies that the root lectin has an extra sequence at its C-terminus. These results not only demonstrate that virtually identical precursor polypeptides are differently processed at their C-terminus in roots and leaves but also indicate that differential processing yields mature lectins with strongly different biological activities. Further screening of the cDNA library for garlic roots also yielded a cDNA clone encoding a protein composed of two tandemly arrayed lectin domains. Since the presumed two-domain root lectin has not been isolated yet, its possible relationship to the previously described two-domain bulb lectin could not be studied at the protein level.
Plant Molecular Biology – Springer Journals
Published: Sep 29, 2004
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