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- Publisher
- Springer Journals
- Copyright
- Copyright © 1987 by Springer-Verlag
- Subject
- Life Sciences; Biotechnology; Agriculture; Biochemistry, general; Plant Biochemistry; Plant Sciences; Plant Genetics & Genomics
- ISSN
- 0040-5752
- eISSN
- 1432-2242
- DOI
- 10.1007/BF00247546
- pmid
- 24240329
- Publisher site
- See Article on Publisher Site
Abstract
122 74 74 6 6 K. C. Engvild Agricultural Research Department Risø National Laboratory DK-4000 Roskilde Denmark Summary The 36 mutants which did not nodulate and 24 mutants which formed inefficient nodules with no or very low acetylene reduction activity were isolated among 86,000 M 2 -seedlings of ‘Finale’ pea, Pisum sativum L., after treatment with chemical mutagens. One mutant was found for approximately every 50 chlorophyll mutants. Most mutations were induced by ethyl methanesulfonate; some by diethyl sulfate, ethyl nitrosourea and acidified sodium azide. Putative mutants were selected as nitrogen deficient plants, yellowing from the bottom and up, when M 2 seedlings were grown in sand with a Rhizobium mixture and PK fertilizer. The mutants were verified in the M 3 generation by acetylene reduction assay on intact plants.
Journal
TAG Theoretical and Applied Genetics – Springer Journals
Published: Oct 1, 1987