Isolation and characterization of abscisic acid‐deficient Arabidopsis mutants at two new loci
Isolation and characterization of abscisic acid‐deficient Arabidopsis mutants at two new loci
Léon‐Kloosterziel, Karen M.; Gil, Marta Alvarez; Ruijs, Gerda J.; Jacobsen, Steven E.; Olszewski, Neil E.; Schwartz, Steven H.; Zeevaart, Jan A.D.; Koornneef, Maarten
1996-10-01 00:00:00
Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA‐deficiency was caused by two monogenic, recessive mutations, aba2 and aba3, that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA‐deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngThe Plant JournalWileyhttp://www.deepdyve.com/lp/wiley/isolation-and-characterization-of-abscisic-acid-deficient-arabidopsis-WFHVuriExx
Isolation and characterization of abscisic acid‐deficient Arabidopsis mutants at two new loci
Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA‐deficiency was caused by two monogenic, recessive mutations, aba2 and aba3, that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA‐deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.
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