Access the full text.
Sign up today, get DeepDyve free for 14 days.
P. Martínez-Culebras, A. Lázaro, P. Campos, C. Jordá (2002)
A RT–PCR Assay Combined with RFLP Analysis for Detection and Differentiation of Isolates of Pepino Mosaic Virus (PepMV) from TomatoEuropean Journal of Plant Pathology, 108
Roger Jones, R. Koenig, D. Lesemann (1980)
Pepino mosaic virus, a new potexvirus from pepino (Solanum muricatum)Annals of Applied Biology, 94
(2000)
Occurrence of Pepino mosaic virus in European tomato crops: identification, etiology and epidemiology
J. Aguilar, M. Hernández-Gallardo, J. Cénis, A. Lacasa, Miguel Aranda (2002)
Complete sequence of the Pepino mosaic virus RNA genomeArchives of Virology, 147
S. Soler, J. Prohens, M. Díez, F. Nuez (2002)
Natural Occurrence of Pepino mosaic virus in Lycopersicon Species in Central and Southern PeruJournal of Phytopathology, 150
Carmen Mansilla, F. Sánchez, F. Ponz (2003)
The Diagnosis of the Tomato Variant of Pepino Mosaic Virus: An IC-RT-PCR ApproachEuropean Journal of Plant Pathology, 109
P. Roggero, V. Masenga, R. Lenzi, F. Coghe, S. Ena, S. Winter (2001)
First report of Pepino mosaic virus in tomato in ItalyPlant Pathology, 50
R. Page (1996)
TreeView: an application to display phylogenetic trees on personal computersComputer applications in the biosciences : CABIOS, 12 4
J. Verhoeven, R. Vlugt, J. Roenhorst (2003)
High Similarity Between Tomato Isolates of Pepino mosaic Virus Suggests a Common OriginEuropean Journal of Plant Pathology, 109
Jai-Hong Cheng, Chi-Weng Peng, Y. Hsu, Ching-Hsiu Tsai (2002)
The Synthesis of Minus-Strand RNA of Bamboo Mosaic Potexvirus Initiates from Multiple Sites within the Poly(A) TailJournal of Virology, 76
C. Jordá, A. Pérez, P. Martínez-Culebras, P. Abad, A. Lacasa, M. Guerrero (2001)
First Report of Pepino mosaic virus on Tomato in Spain.Plant disease, 85 12
A. Cotillon, M. Girard, S. Ducouret (2002)
Complete nucleotide sequence of the genomic RNA of a French isolate of Pepino mosaic virus (PepMV)Archives of Virology, 147
R. Vlugt, C. Stijger, J. Verhoeven, D. Lesemann (2000)
First Report of Pepino Mosaic Virus on Tomato.Plant disease, 84 1
(1997)
Gapped BLAST and PSI-BLAST: A new
C. French, M. Bouthillier, M. Bernardy, G. Ferguson, M. Sabourin, R. Johnson, C. Masters, S. Godkin, R. Mumford (2001)
First Report of Pepino mosaic virus in Canada and the United States.Plant disease, 85 10
(1993)
PHYLIP: Phylogeny Inference Package (ver 3.5c). Distributed by the author. Department of Genetics, University of Washington, Seattle Three distinct US isolates of PepMV
R. Vlugt, M. Berendsen (2002)
Development of a General Potexvirus Detection MethodEuropean Journal of Plant Pathology, 108
C. Jordá, A. Pérez, P. Culebras, A. Lacasa (2001)
First Report of Pepino mosaic virus on Natural Hosts.Plant disease, 85 12
Adrian Gibbs, John Armstrong, Anne Mackenzie, Georg Weiller (1998)
The GPRIME package: computer programs for identifying the best regions of aligned genes to target in nucleic acid hybridisation-based diagnostic tests, and their use with plant viruses.Journal of virological methods, 74 1
L. Baratova, N. Grebenshchikov, E. Dobrov, A. Gedrovich, I. Kashirin, A. Shishkov, A. Efimov, L. Järvekülg, Y. Radavsky, M. Saarma (1992)
The organization of potato virus X coat proteins in virus particles studied by tritium planigraphy and model building.Virology, 188 1
(2003)
Detection and characterization of a US isolate of Pepino mosaic virus
R. Mumford, E. Metcalfe (2001)
The partial sequencing of the genomic RNA of a UK isolate of Pepino mosaic virus and the comparison of the coat protein sequence with other isolates from Europe and PeruArchives of Virology, 146
J. Thompson, D. Higgins, T. Gibson (1994)
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.Nucleic acids research, 22 22
Detlef Gussow, T. Clackson (1989)
Direct clone characterization from plaques and colonies by the polymerase chain reaction.Nucleic acids research, 17 10
Three strains of Pepino mosaic virus (PepMV) found in the US have been cloned and sequenced by RT-PCR using total RNA from infected tissue as template, and degenerate potexvirus- and PepMV species- and isolate-specific primers. Despite limited source material, the complete nucleotide sequences (6413 and 6410 nts, respectively) of two isolates, PepMV-US1 and PepMV-US2, were obtained and analyzed using total RNA from less than 0.2 g of a pooled infected tomato leaf sample from Arizona. Sequence of the 3′-end of the third isolate from infected fresh tomato fruits from Maryland (PepMV-US3) was also determined. The genome organizations of PepMV-US1 and US2 were typical of the genus Potexvirus , with the following reading frame order: ORF 1, encoding a putative replicase; ORFs 2–4, triple gene block proteins (TGBp) 1–3; and ORF 5, coat protein (CP). Gene-for-gene comparison between PepMV-US1 and US2 revealed the following amino acid identities: 91% in replicase, 89% in TGBp1, 92% in TGBp2, 85% in TGBp3, and 93% in the CP; with an overall nucleotide identity of 86%. Nucleotide sequence comparisons between US1 and US2 and the European isolates showed only 79–82% identity, whereas the identity among the European isolates was over 99%. Sequence comparisons and phylogenetic analysis indicate that PepMV-US1 and US2 are distinctly different from the European isolates, while the CP of PepMV-US3 is nearly identical to the European isolates. The results presented also suggest that TGBp1 and TGBp3 are more suitable than either the replicase or coat protein gene products for discriminating PepMV isolates.
Archives of Virology – Springer Journals
Published: Jun 1, 2005
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.