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Binary Vectors for Sense and Antisense Expression of Arabidopsis ESTs

Binary Vectors for Sense and Antisense Expression of Arabidopsis ESTs Our laboratory is interested in devising methods to identify functions for the vast numbers of arabidopsis genes now available. For this purpose, we have constructed a set of binary vectors that will allow the quick production of transgenic arabidopsis plants containing either sense or antisense copies of EST clones obtained from the PRL2 library. These vectors are based on the pSLJ series containing the bialophos resistance (BAR) gene that confers resistance to the herbicide BASTA. In addition, our vectors contain a 35S CaMV promoter-polylinker-nos terminator cassette that allows the direct cloning of arabidopsis ESTs in either antisense (pAOV and pAOV2) or sense (pSOV and pSOV2) orientation. We also describe the construction of two additional vectors conferring BASTA resistance and containing the pBluescript polylinker in both orientations inserted between the 35S CaMV promoter and nos terminator (pKMB and pSMB). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Reporter Springer Journals

Binary Vectors for Sense and Antisense Expression of Arabidopsis ESTs

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References (8)

Publisher
Springer Journals
Copyright
Copyright © 1998 by Kluwer Academic Publishers
Subject
Life Sciences; Plant Sciences; Plant Breeding/Biotechnology; Proteomics; Metabolomics; Bioinformatics
ISSN
0735-9640
eISSN
1572-9818
DOI
10.1023/A:1007520410190
Publisher site
See Article on Publisher Site

Abstract

Our laboratory is interested in devising methods to identify functions for the vast numbers of arabidopsis genes now available. For this purpose, we have constructed a set of binary vectors that will allow the quick production of transgenic arabidopsis plants containing either sense or antisense copies of EST clones obtained from the PRL2 library. These vectors are based on the pSLJ series containing the bialophos resistance (BAR) gene that confers resistance to the herbicide BASTA. In addition, our vectors contain a 35S CaMV promoter-polylinker-nos terminator cassette that allows the direct cloning of arabidopsis ESTs in either antisense (pAOV and pAOV2) or sense (pSOV and pSOV2) orientation. We also describe the construction of two additional vectors conferring BASTA resistance and containing the pBluescript polylinker in both orientations inserted between the 35S CaMV promoter and nos terminator (pKMB and pSMB).

Journal

Plant Molecular Biology ReporterSpringer Journals

Published: Sep 1, 1998

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