Access the full text.
Sign up today, get DeepDyve free for 14 days.
K. Williams (1993)
Ifenprodil discriminates subtypes of the N-methyl-D-aspartate receptor: selectivity and mechanisms at recombinant heteromeric receptors.Molecular pharmacology, 44 4
K. Wafford, Corrinna Bain, B. Bourdellès, P. Whiting, J. Kemp (1993)
Preferential co-assembly of recombinant NMDA receptors composed of three different subunits.Neuroreport, 4 12
C. Carter, J. Benavides, P. Legendre, J. Vincent, F. Noel, F. Thuret, K. Lloyd, S. Arbilla, B. Ẑivković, E. Mackenzie (1988)
Ifenprodil and SL 82.0715 as cerebral anti-ischemic agents. II. Evidence for N-methyl-D-aspartate receptor antagonist properties.The Journal of pharmacology and experimental therapeutics, 247 3
J. Garthwaite, A. Brodbelt (1989)
Synaptic activation of N-methyl-d-aspartate and non-N-methyl-d-aspartate receptors in the mossy fibre pathway in adult and immature rat cerebellar slicesNeuroscience, 29
G. Carmignoto, S. Vicini (1992)
Activity-dependent decrease in NMDA receptor responses during development of the visual cortex.Science, 258 5084
Hamill (1981)
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patchesPflügers Arch., 391
J. Mcdonald, M. Johnston (1990)
Physiological and pathophysiological roles of excitatory amino acids during central nervous system developmentBrain Research Reviews, 15
G. Garthwaite, B. Yamini, J. Garthwaite (1987)
Selective loss of Purkinje and granule cell responsiveness to N-methyl-D-aspartate in rat cerebellum during development.Brain research, 433 2
K. Moriyoshi, M. Masu, T. Ishii, R. Shigemoto, N. Mizuno, S. Nakanishi (1991)
Molecular cloning and characterization of the rat NMDA receptorNature, 354
P. Legendre, G. Westbrook (1991)
Ifenprodil blocks N-methyl-D-aspartate receptors by a two-component mechanism.Molecular pharmacology, 40 2
B. Lambolez, E. Audinat, P. Bochet, F. Crépel, J. Rossier (1992)
AMPA receptor subunits expressed by single purkinje cellsNeuron, 9
D. Laurie, P. Seeburg (1994)
Regional and developmental heterogeneity in splicing of the rat brain NMDAR1 mRNA, 14
K. Ikeda, M. Nagasawa, H. Mori, K. Araki, K. Sakimura, Masahiko Watanabe, Y. Inoue, M. Mishina (1992)
Cloning and expression of the ε4 subunit of the NMDA receptor channelFEBS Letters, 313
P. Chomczyński, N. Sacchi (1987)
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.Analytical biochemistry, 162 1
M. Farrant, D. Feldmeyer, Tomoyuki Takahashi, S. Cull-Candy (1994)
NMDA-receptor channel diversity in the developing cerebellumNature, 368
R. Silver, S. Traynelis, S. Cull-Candy (1992)
Rapid-time-course miniature and evoked excitatory currents at cerebellar synapses in situNature, 355
T. Bliss, G. Collingridge (1993)
A synaptic model of memory: long-term potentiation in the hippocampusNature, 361
H. Monyer, R. Sprengel, R. Schoepfer, A. Herb, M. Higuchi, H. Lomelí, N. Burnashev, B. Sakmann, P. Seeburg (1992)
Heteromeric NMDA Receptors: Molecular and Functional Distinction of SubtypesScience, 256
D. Wyllie, A. Mathie, C Symonds, S. Cull‐Candy (1991)
Activation of glutamate receptors and glutamate uptake in identified macroglial cells in rat cerebellar cultures.The Journal of Physiology, 432
P. Bochet, E. Audinat, B. Lambolez, F. Crépel, J. Rossier, M. Iino, K. Tsuzuki, S. Ozawa (1994)
Subunit composition at the single-cell level explains functional properties of a glutamate-gated channelNeuron, 12
R. Balázs, N. Hack, Ole J∅rgensen (1988)
Stimulation of the N-methyl-d-aspartate receptor has a trophic effect on differentiating cerebellar granule cellsNeuroscience Letters, 87
H. Pearson, M. Graham, R. Burgoyne (1992)
Relationship Between Intracellular Free Calcium Concentration and NMDA‐induced Cerebellar Granule Cell Survival In VitroEuropean Journal of Neuroscience, 4
E. Audinat, T. Knöpfel, B. Gähwiler (1990)
Responses to excitatory amino acids of Purkinje cells' and neurones of the deep nuclei in cerebellar slice cultures.The Journal of Physiology, 430
M. Erlander, N. Tillakaratne, S. Feldblum, Neela Patel, A. Tobin (1991)
Two genes encode distinct glutamate decarboxylasesNeuron, 7
Gähwiler (1984)
Slice culture of cerebellar, hippocampal and hypothalamic tissueExperientia, 40
A. Feinberg, B. Vogelstein (1983)
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.Analytical biochemistry, 132 1
Ian Pearce, M. Cambray-Deakin, R. Burgoyne (1987)
Glutamate acting on NMDA receptors stimulates neurite outgrowth from cerebellar granule cellsFEBS Letters, 223
M. Yamazaki, H. Mori, K. Araki, K. Mori, M. Mishina (1992)
Cloning, expression and modulation of a mouse NMDA receptor subunitFEBS Letters, 300
Y. Bessho, H. Nawa, S. Nakanishi (1994)
Selective up-regulation of an nmda receptor subunit mrna in cultured cerebellar granule cells by K+-induced depolarization and nmda treatmentNeuron, 12
H. Meguro, H. Mori, K. Araki, E. Kushiya, T. Kutsuwada, M. Yamazaki, T. Kumanishi, M. Arakawa, K. Sakimura, M. Mishina (1992)
Functional characterization of a heteromeric NMDA receptor channel expressed from cloned cDNAsNature, 357
Egidio D'Angelo, P. Rossi, Vanni Taglietti (1993)
Different proportions of N-methyl-d-aspartate and non-N-methyl-d-aspartate receptor currents at the mossy fibre-granule cell synapse of developing rat cerebellumNeuroscience, 53
H. Monyer, N. Burnashev, D. Laurie, B. Sakmann, P. Seeburg (1994)
Developmental and regional expression in the rat brain and functional properties of four NMDA receptorsNeuron, 12
G. Collingridge, W. Singer (1990)
Excitatory amino acid receptors and synaptic plasticity.Trends in pharmacological sciences, 11 7
(1987)
82.0715 as cerebral anti-ischemic agents
(1984)
The Cerebellum und Neural Control
D. Rossi, N. Slater (1993)
The developmental onset of NMDA receptor-channel activity during neuronal migrationNeuropharmacology, 32
K. Williams, S. Russell, Yu-Min Shen, P. Molinoff (1993)
Developmental switch in the expression of NMDA receptors occurs in vivo and in vitroNeuron, 10
T. Kutsuwada, N. Kashiwabuchi, H. Mori, K. Sakimura, E. Kushiya, K. Araki, H. Meguro, Hisashi Masaki, T. Kumanishi, M. Arakawa, M. Mishina (1992)
Molecular diversity of the NMDA receptor channelNature, 358
T. Ishii, K. Moriyoshi, H. Sugihara, Kazuhiro Sakurada, H. Kadotani, M. Yokoi, C. Akazawa, Ryuichi ShigemotoS, Noboru MizunoS, M. Masu, Shigetada Nakanishie (1993)
Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits.The Journal of biological chemistry, 268 4
Masahiko Watanabe, Y. Inoue, Kenji Sakimura, Masayoshi Mishina (1992)
Developmental changes in distribution of NMDA receptor channel subunit mRNAs.Neuroreport, 3 12
H. Komuro, P. Rakic (1993)
Modulation of neuronal migration by NMDA receptors.Science, 260 5104
P. Stern, P. Béhé, R. Schoepfer, D. Colquhoun (1992)
Single-channel conductances of NMDA receptors expressed from cloned cDNAs: comparison with native receptorsProceedings of the Royal Society of London. Series B: Biological Sciences, 250
The glutamate receptor channels of the N‐methyl‐d‐aspartate (NMDA) subtype are composed of different subunits named NR1 and NR2A‐D. These subunits can combine in different oligomers with diverging properties and their expression is developmentally regulated. We have used rat cerebellar slice cultures to test the involvement of bioelectrical activity and synaptic transmission in the changes in NR2A‐C expression observed in developing granule cells. A correlation between the functional properties of the NMDA receptors and expression of the NR2A‐C mRNAs was obtained in single granule cells by coupling patch‐clamp recording and reverse transcription followed by polymerase chain reaction. Granule cells grown under standard culture conditions expressed mainly NR2A mRNA when examined after 15–40 days in vitro. Consistent with this observation, their responses to NMDA were only weakly reduced by 3 μM ifenprodil, a non‐competitive antagonist which discriminates between NR2A and NR2B subunits in expression systems. In cerebellar cultures chronically exposed to tetrodotoxin to eliminate spontaneous electrical activity, granule cells maintained a predominant expression of NR2B subunits and their responses to NMDA were largely inhibited by 3 μM ifenprodil. These results provide evidence that the expression of the NR2A and B subunits is regulated through an activity‐dependent mechanism leading to the formation of NMDA receptors with different pharmacological properties. Finally, the NR2C subunit, abundantly expressed in vivo by adult granule cells, was only rarely detected in slice cultures, even when excitatory synapses were formed between granule cells and fibres originating from co‐cultured brainstem explants. These data suggest that the induction of NR2C expression observed in vivo requires an additional factor(s) that remains to be identified.
European Journal of Neuroscience – Wiley
Published: Dec 1, 1994
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.