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K. Mouline, A. Véry, F. Gaymard, Jossia Boucherez, Guillaume Pilot, M. Devic, D. Bouchez, J. Thibaud, H. Sentenac (2002)
Pollen tube development and competitive ability are impaired by disruption of a Shaker K(+) channel in Arabidopsis.Genes & development, 16 3
D. Preuss, B. Lemieux, Grace Yen, R. Davis (1993)
A conditional sterile mutation eliminates surface components from Arabidopsis pollen and disrupts cell signaling during fertilization.Genes & development, 7 6
S. Hashida, Hideyuki Takahashi, M. Kawai‐Yamada, H. Uchimiya (2007)
Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltransferase (AtNMNAT) is required for pollen tube growth.The Plant journal : for cell and molecular biology, 49 4
D. Honys, D. Twell (2003)
Comparative Analysis of the Arabidopsis Pollen Transcriptome
G.P. Copenhaver, K.C. Keith, D. Preuss (2000)
Tetrad analysis in higher plants. A budding technologyJ. Bioenerg. Biomembr., 124
C. Pina, F. Pinto, J.A. Feijo, J.D. Becker (2005)
Gene family analysis of the Arabidopsis pollen transcriptome reveals biological implications for cell growth, division control, and gene expression regulationField Crops Res., 138
K. Mouline, A.A. Very, F. Gaymard, J. Boucherez, G. Pilot, M. Devic, D. Bouchez, J.B. Thibaud, H. Sentenac (2002)
Pollen tube development and competitive ability are impaired by disruption of a Shaker K(+) channel in ArabidopsisPhysiol Plant., 16
K.R. Shivanna, H.F. Linskens, M. Cresti (1991)
Pollen viability and pollen vigorPlant Cell, 81
S.A. Johnson‐Brousseau, S. McCormick (2004)
A compendium of methods useful for characterizing Arabidopsis pollen mutants and gametophytically‐expressed genesAnn. Bot., 39
L. Young, R. Wilen, P. Bonham‐Smith (2004)
High temperature stress of Brassica napus during flowering reduces micro- and megagametophyte fertility, induces fruit abortion, and disrupts seed production.Journal of experimental botany, 55 396
Y.F. Wang, L.M. Fan, W.Z. Zhang, W. Zhang, W.H. Wu (2004)
Ca2+‐permeable channels in the plasma membrane of Arabidopsis pollen are regulated by actin microfilamentsJ. Exp. Bot., 136
A. Procissi, A. Guyon, E.S. Pierson, A. Giritch, B. Knuiman, O. Grandjean, C. Tonelli, J. Derksen, G. Pelletier, S. Bonhomme (2003)
KINKY POLLEN encodes a SABRE‐like protein required for tip growth in Arabidopsis and conserved among eukaryotesSci. STKE, 36
G. Copenhaver, K. Keith, D. Preuss (2000)
Tetrad analysis in higher plants. A budding technology.Plant physiology, 124 1
J. Feijó, J. Sainhas, T. Holdaway-Clarke, M. Cordeiro, J. Kunkel, P. Hepler (2000)
Cellular oscillations and the regulation of growth: the pollen tube paradigmBioEssays, 23
H. Pasonen, M. Käpylä (1998)
Pollen–pollen interactions in Betula pendula in vitroNew Phytologist, 138
J. Exp. Bot
Azarov (1990)
9Arabidopsis Inf. Serv., 27
J. Brewbaker, S. Majumder (1961)
CULTURAL STUDIES OF THE POLLEN POPULATION EFFECT AND THE SELF‐INCOMPATIBILITY INHIBITIONAmerican Journal of Botany, 48
J. Heslop-Harrison, Y. Heslop-Harrison, K. Shivanna (1984)
The evaluation of pollen quality, and a further appraisal of the fluorochromatic (FCR) test procedureTheoretical and Applied Genetics, 67
T. Murashige, F. Skoog (1962)
A revised medium for rapid growth and bio assays with tobacco tissue culturesPhysiologia Plantarum, 15
L. Taylor, P. Hepler (1997)
POLLEN GERMINATION AND TUBE GROWTH.Annual review of plant physiology and plant molecular biology, 48
K. Robinson, M. Messerli (2002)
Pulsating Ion Fluxes and Growth at the Pollen Tube TipScience's STKE, 2002
D. Honys, D. Twell (2004)
Transcriptome analysis of haploid male gametophyte development in ArabidopsisGenome Biology, 5
A. Procissi, A. Guyon, Elisabeth Pierson, A. Giritch, B. Knuiman, O. Grandjean, Chiara Tonelli, Jan Derksen, Georges Pelletier, Sandrine Bonhomme (2003)
KINKY POLLEN encodes a SABRE-like protein required for tip growth in Arabidopsis and conserved among eukaryotes.The Plant journal : for cell and molecular biology, 36 6
J. Heslop‐Harrison, Y. Heslop‐Harrison, K.R. Shivanna (1984)
The evaluation of pollen quality, and a further appraisal of the fluorochromatic (Fcr) test procedurePlant Physiol., 67
J. Mascarenhas (1993)
Molecular Mechanisms of Pollen Tube Growth and Differentiation.The Plant cell, 5
Yong-Fei Wang, L. Fan, Wen-Zheng Zhang, Wei Zhang, Wei-Hua Wu (2004)
Ca2+-Permeable Channels in the Plasma Membrane of Arabidopsis Pollen Are Regulated by Actin Microfilaments1Plant Physiology, 136
J.H. Crowe, L.M. Crowe, F.A. Hoekstra (1989)
Phase transitions and permeability changes in dry membranes during rehydrationAnnu. Rev. Physiol., 21
Sheila Johnson-Brousseau, S. McCormick (2004)
A compendium of methods useful for characterizing Arabidopsis pollen mutants and gametophytically-expressed genes.The Plant journal : for cell and molecular biology, 39 5
J. Becker, Leonor Boavida, J. Carneiro, M. Haury, J. Feijó (2003)
Transcriptional Profiling of Arabidopsis Tissues Reveals the Unique Characteristics of the Pollen Transcriptome1[w]Plant Physiology, 133
M.A. Messerli, K.R. Robinson (1998)
Cytoplasmic acidification and current influx follow growth pulses of Lilium longiflorum pollen tubesJ. Cell Sci., 16
M. Messerli, K. Robinson (1998)
Cytoplasmic acidification and current influx follow growth pulses of Lilium longiflorum pollen tubesPlant Journal, 16
M.P. Herrero, R.R. Johnson (1980)
High‐temperature stress and pollen viability of maizeTheor. Appl. Genet., 20
A. Larden, A. Triboi-Blondel (1994)
Freezing injury to ovules, pollen and seeds in winter rapeJournal of Experimental Botany, 45
The Initiative (2000)
Analysis of the genome sequence of the flowering plant Arabidopsis thalianaNature, 408
S.N. Hashida, H. Takahashi, M. Kawai‐Yamada, H. Uchimiya (2007)
Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltransferase (AtNMNAT) is required for pollen tube growthAnn. Rev. Cell Dev. Biol., 49
H.L. Pasonen, M. Kapyla (1998)
Pollen–pollen interactions in Betula pendula in vitroArabidopsis Inf. Serv., 138
Y.‐F. Chen, Y. Matsubayashi, Y. Sakagami (2000)
Peptide growth factor phytosulfokine-α contributes to the pollen population effectPlanta, 211
P. Hepler, L. Vidali, A. Cheung (2001)
Polarized cell growth in higher plants.Annual review of cell and developmental biology, 17
R. Cerović, D. Ružić, N. Micic (2000)
Viability of plum ovules at different temperaturesAnnals of Applied Biology, 137
A.Y. Cheung, H.M. Wu (2007)
Structural and functional compartmentalization in pollen tubesField Crops Res., 58
S. Mansfield, L. Briarty (1991)
Early embryogenesis in Arabidopsis thaliana. II. The developing embryoBotany, 69
J. Bioenerg. Biomembr
S. Sato, M. Kamiyama, T. Iwata, N. Makita, H. Furukawa, H. Ikeda (2006)
Moderate increase of mean daily temperature adversely affects fruit set of Lycopersicon esculentum by disrupting specific physiological processes in male reproductive developmentPlant Physiol., 97
A. Lardon, A.M. Triboiblondel (1994)
Freezing‐injury to ovules, pollen and seeds in winter rapePlant Cell, 45
K. Shivanna, J. Heslop-Harrison (1981)
Membrane state and pollen viabilityAnnals of Botany, 47
(1981)
Effect of high-temperature on pollen grain germination, pollen-tube growth, and seed yield of chinese cabbage
D.P. Singh, A.M. Jermakow, S.M. Swain (2002)
Gibberellins are required for seed development and pollen tube growth in ArabidopsisPlant Physiol., 14
J. Derksen, B. Knuiman, K. Hoedemaekers, A. Guyon, S. Bonhomme, E.S. Pierson (2002)
Growth and cellular organization of Arabidopsis pollen tubes in vitroJ. Exp. Bot., 15
M. Messerli, Gaudenz Danuser, Kenneth Robinson (1999)
Pulsatile influxes of H+, K+ and Ca2+ lag growth pulses of Lilium longiflorum pollen tubes.Journal of cell science, 112 ( Pt 10)
N. Kozai, K. Beppu, R. Mochioka, U. Boonprakob, S. Subhadrabandhu, I. Kataoka (2004)
Adverse effects of high temperature on the development of reproductive organs in ‘Hakuho’ peach treesThe Journal of Horticultural Science and Biotechnology, 79
M. Morrison (1993)
Heat stress during reproduction in summer rapeBotany, 71
(2005)
Genetic dissection of cell-to-cell interactions in sexual reproduction in Arabidopsis thaliana
P.K. Hepler, L. Vidali, A.Y. Cheung (2001)
Polarized cell growth in higher plantsCrop Sci., 17
K. Shivanna, H. Linskens, M. Cresti (2004)
Pollen viability and pollen vigorTheoretical and Applied Genetics, 81
A. Cheung, Hen-Ming Wu (2006)
Structural and functional compartmentalization in pollen tubes.Journal of experimental botany, 58 1
P. Taylor, J. Glover, M. Lavithis, S. Craig, M. Singh, R. Knox, E. Dennis, A. Chaudhury (1998)
Genetic control of male fertility in Arabidopsis thaliana: structural analyses of postmeiotic developmental mutantsPlanta, 205
Rex Cole, Lukáš Synek, V. Žárský, J. Fowler (2005)
SEC8, a Subunit of the Putative Arabidopsis Exocyst Complex, Facilitates Pollen Germination and Competitive Pollen Tube Growth1[w]Plant Physiology, 138
Y. Chen, Y. Matsubayashi, Y. Sakagami (2000)
Peptide growth factor phytosulfokine-alpha contributes to the pollen population effect.Planta, 211 5
H. Jakobsen, H. Martens (1994)
Influence of Temperature and Ageing of Ovules and Pollen on Reproductive Success in Trifolium repens L.Annals of Botany, 74
J. Brewbaker (1967)
THE DISTRIBUTION AND PHYLOGENETIC SIGNIFICANCE OF BINUCLEATE AND TRINUCLEATE POLLEN GRAINS IN THE ANGIOSPERMSAmerican Journal of Botany, 54
José Feijó, J. Sainhas, G. Hackett, Joseph Kunkel, P. Hepler (1999)
Growing Pollen Tubes Possess a Constitutive Alkaline Band in the Clear Zone and a Growth-dependent Acidic TipThe Journal of Cell Biology, 144
M.A. Messerli, G. Danuser, K.R. Robinson (1999)
Pulsatile influxes of H+, K+ and Ca2+ lag growth pulses of Lilium longiflorum pollen tubesCan. J. Bot., 112 (Pt 10)
A.S. Azarov, B.I. Tokarev, A.E. Netchepurenko (1990)
Effect of sodium chloride on pollen germination and pollen tube growth in vitro in Arabidopsis thaliana (L.) HeynhArabidopsis Inf. Serv., 27
L. Fan, Yong-Fei Wang, Hong Wang, Wei-Hua Wu (2001)
In vitro Arabidopsis pollen germination and characterization of the inward potassium currents in Arabidopsis pollen grain protoplasts.Journal of experimental botany, 52 361
I. Steinebrunner, Jian Wu, Yu Sun, A. Corbett, S. Roux (2003)
Disruption of Apyrases Inhibits Pollen Germination in Arabidopsis1Plant Physiology, 131
H. Clarke, K. Siddique (2004)
Response of chickpea genotypes to low temperature stress during reproductive developmentField Crops Research, 90
M. Herrero, R. Johnson (1980)
High Temperature Stress and Pollen Viability of Maize 1Crop Science, 20
J. Derksen, B. Knuiman, Karin Hoedemaekers, A. Guyon, S. Bonhomme, E. Pierson (2002)
Growth and cellular organization of Arabidopsis pollen tubes in vitroSexual Plant Reproduction, 15
(2007)
The Plant Journal
Hai Li, Yakang Lin, Rachel Heath, M. Zhu, Zhenbiao Yang (1999)
Control of Pollen Tube Tip Growth by a Rop GTPase–Dependent Pathway That Leads to Tip-Localized Calcium InfluxPlant Cell, 11
J. Brewbaker, S.K. Majumder (1961)
Cultural studies of pollen population effect and self‐incompatibility inhibitionAnn. Appl. Biol., 48
José Feijó, Rui Malhó, G. Obermeyer (1995)
Ion dynamics and its possible role during in vitro pollen germination and tube growthProtoplasma, 187
J. Crowe, L. Crowe, F. Hoekstra (1989)
Phase transitions and permeability changes in dry membranes during rehydrationJournal of Bioenergetics and Biomembranes, 21
P.V.V. Prasad, K.J. Boote, L.H. Allen, J.E. Sheehy, J.M.G. Thomas (2006)
Species, ecotype and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature stressGenes Dev., 95
V.G. Kakani, K.R. Reddy, S. Koti, T.P. Wallace, P.V.V. Prasad, V.R. Reddy, D. Zhao (2005)
Differences in in vitro pollen germination and pollen tube growth of cotton cultivars in response to high temperatureJ. Hort. Sci. Biotech., 96
Daphne Preuss, Seung Rhee, Ronald Davis (1994)
Tetrad analysis possible in Arabidopsis with mutation of the QUARTET (QRT) genes.Science, 264 5164
(1988)
In vitro germination and storage of trinucleate Arabidopsis thaliana (L.) pollen grains
Plant Physiol
S. Sato, M. Kamiyama, T. Iwata, N. Makita, H. Furukawa, H. Ikeda (2006)
Moderate increase of mean daily temperature adversely affects fruit set of Lycopersicon esculentum by disrupting specific physiological processes in male reproductive development.Annals of botany, 97 5
V. Kakani, K. Reddy, S. Koti, T. Wallace, P. Prasad, V. Reddy, Duli Zhao (2005)
Differences in in vitro pollen germination and pollen tube growth of cotton cultivars in response to high temperature.Annals of botany, 96 1
(2003)
AtSTP 6 , a New Pollen-Specific H-Monosaccharide Symporter from Arabidopsis 1
H.B. Jakobsen, H. Martens (1994)
Influence of temperature and aging of ovules and pollen on reproductive success in Trifolium repens LPlant J., 74
P. Prasad, K. Boote, L. Allen, J. Sheehy, Jean Thomas (2006)
Species, ecotype and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature stressField Crops Research, 95
D. Honys, D. Twell (2004)
Transcriptome analysis of haploid male gametophyte development in ArabidopsisAnn. Bot., 5
C. Pina, F. Pinto, J. Feijó, J. Becker (2005)
Gene Family Analysis of the Arabidopsis Pollen Transcriptome Reveals Biological Implications for Cell Growth, Division Control, and Gene Expression Regulation1[w]Plant Physiology, 138
H.J. Clarke, K.H.M. Siddique (2004)
Response of chickpea genotypes to low temperature stress during reproductive developmentPlant Physiol., 90
R. Arteca, J. Arteca (2000)
A novel method for growing Arabidopsis thaliana plants hydroponicallyPhysiologia Plantarum, 108
M. Thorsness, M. Kandasamy, M. Nasrallah, J. Nasrallah (1993)
Genetic Ablation of Floral Cells in Arabidopsis.The Plant cell, 5
D. Singh, A. Jermakow, S. Swain (2002)
Gibberellins Are Required for Seed Development and Pollen Tube Growth in Arabidopsis Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.003046.The Plant Cell Online, 14
M.K. Thorsness, M.K. Kandasamy, M.E. Nasrallah, J.B. Nasrallah (1993)
Genetic ablation of floral cells in ArabidopsisPlant Physiol., 5
J.H. Crowe, F.A. Hoekstra, L.M. Crowe (1992)
AnhydrobiosisSex. Plant Reprod., 54
J. Scholz-Starke, M. Büttner, N. Sauer (2003)
AtSTP6, a New Pollen-Specific H+-Monosaccharide Symporter from Arabidopsis1Plant Physiology, 131
K.R. Shivanna, J. Heslop‐Harrison (1981)
Membrane state and pollen viabilityTheor. Appl. Genet., 47
I. Steinebrunner, J. Wu, Y. Sun, A. Corbett, S.J. Roux (2003)
Disruption of apyrases inhibits pollen germination in ArabidopsisAnnu. Rev. Plant Physiol. Plant Mol. Biol., 131
T. Murashige, F. Skoog (1962)
A revised medium for rapid growth and bioassays with tobacco tissue culturesNew Phytol., 15
H. Li, Y. Lin, R.M. Heath, M.X. Zhu, Z. Yang (1999)
Control of pollen tube tip growth by a Rop GTPase‐dependent pathway that leads to tip‐localized calcium influxCan. J. Bot., 11
(1990)
Effect of sodium chloride on pollen germination and pollen tube growth in vitro in Arabidopsis thaliana (L.) Heynh. Arabidopsis Inf
Despite much effort, a robust protocol for in vitro germination of Arabidopsis thaliana pollen has been elusive. Here we show that controlled temperatures, a largely disregarded factor in previous studies, and a simple optimized medium, solidified or liquid, yielded pollen germination rates above 80% and pollen tube lengths of hundreds of microns, with both Columbia and Landsberg erecta (Ler) ecotypes. We found that pollen germination and tube growth were dependent on pollen density in both liquid and solid medium. Pollen germination rates were not substantially affected by flower or plant age. The quartet1 mutation negatively affected pollen germination, especially in the Ler ecotype. This protocol will facilitate functional analyses of insertional mutants affecting male gametophyte function, and should allow detailed gene expression analyses during pollen tube growth. Arabidopsis thaliana can now be included on the list of plant species that are suitable models for physiological studies of pollen tube elongation and tip growth.
The Plant Journal – Wiley
Published: Nov 1, 2007
Keywords: ; ; ; ; ;
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