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TMV resistance gene N homologues are linked to Synchytrium endobioticum resistance in potato

TMV resistance gene N homologues are linked to Synchytrium endobioticum resistance in potato The fungus Synchytrium endobioticum , the causal agent of potato wart disease, is subject to world-wide quarKantine regulations due to the production of persistent resting spores and lack of effective chemical control measures. The selection of Synchytrium- resistant potato cultivars may be facilitated by using markers closely linked with a resistance gene or by transferring a cloned gene for resistance into susceptible cultivars. Sen1 , a gene for resistance to Synchytrium endobioticum race 1, was localized on potato chromosome XI in a genomic region which is related to the tobacco genome segment harbouring the N gene for resistance to TMV. Using N as probe, we isolated homologous cDNA clones from a Synchytrium- resistant potato line. The N -homologous sequences of potato identified by RFLP mapping a family of resistance gene-like sequences closely linked with the Sen1 locus. Sequence analysis of two full-length N -homologous cDNA clones revealed the presence of structural domains associated with resistance gene function. One clone (Nl-25) encodes a polypeptide of 61 kDa and harbours a Toll-interleukin like region (TIR) and a putative nucleotide binding site (NBS). The other clone (Nl-27) encodes a polypeptide of 95 kDa and harbours besides the TIR and NBS domains five imperfect leucine-rich repeats (LRRs). Both clones have at their amino terminus a conserved stretch of serine residues that was also found in the N gene, the RPP5 gene from Arabidopsis thaliana and several other resistance gene homologues, suggesting a function in the resistance response. Cloning of the disease resistance locus based on map position and the establishment of PCR-based marker assays to assist selection of wart resistant potato genotypes are discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png TAG Theoretical and Applied Genetics Springer Journals

TMV resistance gene N homologues are linked to Synchytrium endobioticum resistance in potato

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References (35)

Publisher
Springer Journals
Copyright
Copyright © 1999 by Springer-Verlag Berlin Heidelberg
Subject
Legacy
ISSN
0040-5752
eISSN
1432-2242
DOI
10.1007/s001220051083
Publisher site
See Article on Publisher Site

Abstract

The fungus Synchytrium endobioticum , the causal agent of potato wart disease, is subject to world-wide quarKantine regulations due to the production of persistent resting spores and lack of effective chemical control measures. The selection of Synchytrium- resistant potato cultivars may be facilitated by using markers closely linked with a resistance gene or by transferring a cloned gene for resistance into susceptible cultivars. Sen1 , a gene for resistance to Synchytrium endobioticum race 1, was localized on potato chromosome XI in a genomic region which is related to the tobacco genome segment harbouring the N gene for resistance to TMV. Using N as probe, we isolated homologous cDNA clones from a Synchytrium- resistant potato line. The N -homologous sequences of potato identified by RFLP mapping a family of resistance gene-like sequences closely linked with the Sen1 locus. Sequence analysis of two full-length N -homologous cDNA clones revealed the presence of structural domains associated with resistance gene function. One clone (Nl-25) encodes a polypeptide of 61 kDa and harbours a Toll-interleukin like region (TIR) and a putative nucleotide binding site (NBS). The other clone (Nl-27) encodes a polypeptide of 95 kDa and harbours besides the TIR and NBS domains five imperfect leucine-rich repeats (LRRs). Both clones have at their amino terminus a conserved stretch of serine residues that was also found in the N gene, the RPP5 gene from Arabidopsis thaliana and several other resistance gene homologues, suggesting a function in the resistance response. Cloning of the disease resistance locus based on map position and the establishment of PCR-based marker assays to assist selection of wart resistant potato genotypes are discussed.

Journal

TAG Theoretical and Applied Genetics – Springer Journals

Published: Mar 1, 1999

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