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Neurotrophin‐3, but not nerve growth factor, promotes survival of human intestinal mast cells

Neurotrophin‐3, but not nerve growth factor, promotes survival of human intestinal mast cells Abstract Neurotrophins are potent regulators of neuronal cell survival and function. Nerve growth factor (NGF) was shown to reduce apoptosis in cord blood‐derived mast cells. Here, we examined the effect of the neurotrophins NGF and neurotrophin (NT)‐3 on survival and mediator release of human intestinal mast cells. Mast cells isolated from normal intestinal tissue were cultured in the presence of NGF, NT‐3, or stem cell factor (SCF) alone or in the presence of SCF together with each neurotrophin. NGF or NT‐3 alone did not promote mast cell survival. In contrast, mast cell recovery was increased twofold when mast cells were cultured with NT‐3 in addition to SCF for 14 days compared with control. Mast cell recovery was further increased following a combined addition of NT‐3, SCF and IL‐4. NT‐3 mediated mast cell growth was dependent on the primary receptor for NT‐3 TrkC. NGF in combination with SCF or with SCF and IL‐4 showed no effect on mast cell survival. Histamine release and histamine content per mast cell remained unchanged, whereas leukotriene C4 release decreased if mast cells were cultured with NGF or NT‐3 in addition to SCF. In summary, NT‐3 affects mature human mast cells by promoting mast cell survival, whereas NGF does not. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neurogastroenterology & Motility Wiley

Neurotrophin‐3, but not nerve growth factor, promotes survival of human intestinal mast cells

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References (42)

Publisher
Wiley
Copyright
Copyright © 2007 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1350-1925
eISSN
1365-2982
DOI
10.1111/j.1365-2982.2007.00899.x
pmid
17391246
Publisher site
See Article on Publisher Site

Abstract

Abstract Neurotrophins are potent regulators of neuronal cell survival and function. Nerve growth factor (NGF) was shown to reduce apoptosis in cord blood‐derived mast cells. Here, we examined the effect of the neurotrophins NGF and neurotrophin (NT)‐3 on survival and mediator release of human intestinal mast cells. Mast cells isolated from normal intestinal tissue were cultured in the presence of NGF, NT‐3, or stem cell factor (SCF) alone or in the presence of SCF together with each neurotrophin. NGF or NT‐3 alone did not promote mast cell survival. In contrast, mast cell recovery was increased twofold when mast cells were cultured with NT‐3 in addition to SCF for 14 days compared with control. Mast cell recovery was further increased following a combined addition of NT‐3, SCF and IL‐4. NT‐3 mediated mast cell growth was dependent on the primary receptor for NT‐3 TrkC. NGF in combination with SCF or with SCF and IL‐4 showed no effect on mast cell survival. Histamine release and histamine content per mast cell remained unchanged, whereas leukotriene C4 release decreased if mast cells were cultured with NGF or NT‐3 in addition to SCF. In summary, NT‐3 affects mature human mast cells by promoting mast cell survival, whereas NGF does not.

Journal

Neurogastroenterology & MotilityWiley

Published: Apr 1, 2007

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