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GT‐1 belongs to the class of trihelix DNA‐binding proteins and binds to a promoter sequence found in many different genes. Data presented in this report show that GT‐1 contains a trans‐activation function in yeast and in plant cells. However, in tobacco BY‐2 protoplasts, this activity functions only when an internal region containing the DNA‐binding domain is deleted. Gel‐shift and co‐immunoprecipitation assays have revealed that GT‐1 can interact with and stabilize the TFIIA–TBP–TATA complex. These results suggest that GT‐1 may activate transcription through direct inter‐ action with the transcriptional pre‐initiation complex.
The Plant Journal – Wiley
Published: Jun 1, 1999
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