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The singlet oxygen quantum yield (ϕ1o2) of 11 purified fluorescein derivatives was determined by reaction with singlet oxygen acceptors in aqueous and ethanolic solutions; in both solvents ϕ1o2 was enhanced with increasing halogenation. Tryptophan and 2,2,6,6‐tetramethylpiperidone were found to be unadapted for the determination of ϕ1o2, in our systems; however, the use of 9.10‐dipropionic anthracene acid andp‐nitrosodimethylaniline in conjunction with imidazole derivatives was suitable for 1O2 detection in water. Both methods lead to results in excellent agreement. As in ethanol. ϕ1o2, was equal to the triplet state quantum yield (ϕT), the comparison between the two solvents showed that ϕT in water was greater than in ethanol. The comparison between our values obtained with polychromatic light with published data obtained with monochromatic light suggests that the triplet quantum yield of fluorescein derivatives is wavelength independent.
Photochemistry & Photobiology – Wiley
Published: Mar 1, 1983
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