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Spatio‐temporal analysis of mitotic activity with a labile cyclin–GUS fusion protein

Spatio‐temporal analysis of mitotic activity with a labile cyclin–GUS fusion protein Plant growth responds rapidly to developmental and environmental signals, but the underlying changes in cell division activity are poorly understood. A labile cyclin‐GUS reporter was developed to facilitate the spatio‐temporal analysis of cell division patterns. The chimeric reporter protein is turned over every cell cycle and hence its histochemical activity accurately reports individual mitotic cells. Using Arabidopsis plants transformed with cyclin‐GUS, we visualized patterns of mitotic activity in wounded leaves which suggest a role for cell division in structural reinforcement. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

Spatio‐temporal analysis of mitotic activity with a labile cyclin–GUS fusion protein

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References (18)

Publisher
Wiley
Copyright
Copyright © 1999 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
DOI
10.1046/j.1365-313x.1999.00620.x
Publisher site
See Article on Publisher Site

Abstract

Plant growth responds rapidly to developmental and environmental signals, but the underlying changes in cell division activity are poorly understood. A labile cyclin‐GUS reporter was developed to facilitate the spatio‐temporal analysis of cell division patterns. The chimeric reporter protein is turned over every cell cycle and hence its histochemical activity accurately reports individual mitotic cells. Using Arabidopsis plants transformed with cyclin‐GUS, we visualized patterns of mitotic activity in wounded leaves which suggest a role for cell division in structural reinforcement.

Journal

The Plant JournalWiley

Published: Nov 1, 1999

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