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R. Wyse, Ewald Komor (1984)
Mechanism of amino Acid uptake by sugarcane suspension cells.Plant physiology, 76 4
T. Taji, C. Ohsumi, S. Iuchi, M. Seki, M. Kasuga, Masatomo Kobayashi, K. Yamaguchi-Shinozaki, K. Shinozaki (2002)
Important roles of drought- and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana.The Plant journal : for cell and molecular biology, 29 4
K. Breitkreuz, B. Shelp, Wolf Fischer, R. Schwacke, D. Rentsch (1999)
Identification and characterization of GABA, proline and quaternary ammonium compound transporters from Arabidopsis thalianaFEBS Letters, 450
B. Hirner, Wolf Fischer, Doris Rentsch, M. Kwart, Wolf Frommer (1998)
Developmental control of H+/amino acid permease gene expression during seed development of Arabidopsis.The Plant journal : for cell and molecular biology, 14 5
F. Chaudhry, R. Reimer, D. Križaj, D. Barber, J. Storm-Mathisen, D. Copenhagen, R. Edwards (1999)
Molecular Analysis of System N Suggests Novel Physiological Roles in Nitrogen Metabolism and Synaptic TransmissionCell, 99
H. Lam, K. Coschigano, C. Schultz, Rosana Melo-Oliveira, G. Tjaden, I. Oliveira, Nora Ngai, M. Hsieh, G. Coruzzi (1995)
Use of Arabidopsis mutants and genes to study amide amino acid biosynthesis.The Plant cell, 7
J.C. Jauniaux, M. Grenson (1990)
GAP1, the general amino acid permease gene of Saccharomyces cerevisiae. Nucleotide sequence, protein similarity with the other bakers yeast amino acid permeases, and nitrogen catabolite repressionPlant Physiol., 29
J. Harper, L. Manney, M. Sussman (1994)
The plasma membrane H+-ATPase gene family in Arabidopsis: genomic sequence of AHA10 which is expressed primarily in developing seedsMolecular and General Genetics MGG, 244
M. Sugawara, T. Nakanishi, Y.J. Fei, W. Huang, M.E. Ganapathy, F.H. Leibach, V. Ganapathy (2000)
Cloning of an amino acid transporter with functional characteristics and tissue expression pattern identical to that of system APlant Sci. Lett., 29
G. Lohaus, H. Winter, B. Riens, H. Heldt (1995)
Further Studies of the Phloem Loading Process in Leaves of Barley and Spinach. The Comparison of Metabolite Concentrations in the Apoplastic Compartment with those in the Cytosolic Compartment and in the Sieve Tubes1, 108
P. Wipf, U. Ludewig, M. Tegeder, D. Rentsch, W Koch, W.B. Frommer (2002)
Conservation of amino acid transporters in fungi, plants and animalPlant Physiol., 29
D. Bush (1993)
Proton-Coupled Sugar and Amino Acid Transporters in Plants, 44
H. Pfanz, K.J. Dietz (1987)
A fluorescence method for the determination of the apoplastic proton concentration in intact leaf tissuesProc. Natl Acad. Sci. USA, 29
M. Bennett, A. Marchant, Haydn Green, S. May, Sally Ward, P. Millner, A. Walker, B. Schulz, K. Feldmann (1996)
Arabidopsis AUX1 Gene: A Permease-Like Regulator of Root GravitropismScience, 273
A. Bel (1984)
Quantification of the xylem-to-phloem transfer of amino acids by use of inulin [14C]carboxylic acid as xylem transport markerPlant Science Letters, 35
H. Kikuchi, D. Fujimoto (1973)
Multiplicity of histone deacetylase from calf thymusFEBS Letters, 29
D. Rentsch, B. Hirner, E. Schmelzer, W.B. Frommer (1996)
Salt stress‐induced proline transporters and salt stress‐repressed broad specificity amino acid permeases identified by suppression of a yeast amino acid permease‐targeting mutantPlant Physiol., 29
B. Hirner, W.N. Fischer, D. Rentsch, M. Kwart, W.B. Frommer (1998)
Developmental control of H+/amino acid permease gene expression during seed development of ArabidopsisEur. J. Biochem., 29
K. Weston, J. Hall, L. Williams (1995)
Characterization of amino-acid transport in Ricinus communis roots using isolated membrane vesiclesPlanta, 196
N. Dewitt, B. Hong, M. Sussman, J. Harper (1996)
Targeting of Two Arabidopsis H+-ATPase Isoforms to the Plasma Membrane, 112
B. Riens, G. Lohaus, D. Heineke, H.W. Heldt (1991)
Amino acid and sucrose content determined in the cytosolic, chloroplastic, and vacuolar compartments and in the phloem sap of spinach leavesJ. Biol. Chem., 29
K. Boorer, Wolf Fischer (1997)
Specificity and Stoichiometry of the ArabidopsisH+/Amino Acid Transporter AAP5*The Journal of Biological Chemistry, 272
Wolf Fischer, M. Kwart, S. Hummel, W. Frommer (1995)
Substrate Specificity and Expression Profile of Amino Acid Transporters (AAPs) in Arabidopsis(*)The Journal of Biological Chemistry, 270
Wolf Frommer, S. Hummel, J. Riesmeier (1993)
Expression cloning in yeast of a cDNA encoding a broad specificity amino acid permease from Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America, 90 13
A. Marchant, J. Kargul, S. May, P. Muller, A. Delbarre, C. Perrot-Rechenmann, M. Bennett (1999)
AUX1 regulates root gravitropism in Arabidopsis by facilitating auxin uptake within root apical tissuesThe EMBO Journal, 18
Z. Li, D. Bush (1992)
Structural determinants in substrate recognition by proton-amino acid symports in plasma membrane vesicles isolated from sugar beet leaves.Archives of biochemistry and biophysics, 294 2
T. Kinraide, B. Etherton (1980)
Electrical evidence for different mechanisms of uptake for basic, neutral, and acidic amino acids in oat coleoptiles.Plant physiology, 65 6
B. Riens, G. Lohaus, D. Heineke, H. Heldt (1991)
Amino Acid and sucrose content determined in the cytosolic, chloroplastic, and vacuolar compartments and in the Phloem sap of spinach leaves.Plant physiology, 97 1
M. Kwart, B. Hirner, S. Hummel, Wolf Frommer (1993)
Differential expression of two related amino acid transporters with differing substrate specificity in Arabidopsis thaliana.The Plant journal : for cell and molecular biology, 4 6
J. Allen, S. Elledge (1994)
A family of vectors that facilitate transposon and insertional mutagenesis of cloned genes in yeastYeast, 10
G. Lohaus, M. Burba, H. Heldt (1994)
Comparison of the contents of sucrose and amino acids in the leaves, phloem sap and taproots of high and low sugar-producing hybrids of sugar beet (Beta vulgaris L.)Journal of Experimental Botany, 45
G. Galili, O. Shaul, A. Perl, B. Karchi (1995)
Seed Development and GerminationMol. Gen. Genet.
Zhen-Chang Li, Daniel Bush (1990)
DeltapH-Dependent Amino Acid Transport into Plasma Membrane Vesicles Isolated from Sugar Beet Leaves: I. Evidence for Carrier-Mediated, Electrogenic Flux through Multiple Transport Systems.Plant physiology, 94 1
D.D. Loo, T. Zeuthen, G. Chandy, E.M. Wright (1996)
Cotransport of water by the Na+/glucose cotransporterEMBO J., 29
G. Lohaus, M. Burba, H.W. Heldt (1994)
Comparison of the contents of sucrose and amino acids in the leaves, phloem sap and taproots of high and low sugar‐producing hybrids of sugar beet (Beta vulgaris L.)Bot. Acta, 29
Z.C. Li, D.R. Bush (1990)
ΔpH‐Dependent amino acid transport into plasma membrane vesicles isolated form sugar beet leaves. I. Evidence for carrier‐mediated electronic flux through multiple leavesPlant Physiol., 29
A. Marchant, J. Kargul, S.T. May, P. Muller, A. Delbarre, C. Perrot‐Rechenmann, M.J. Bennett (1999)
AUX1 regulates root gravitropism in Arabidopsis by facilitating auxin uptake within root apical tissuesPlant J., 29
D. Wipf, U. Ludewig, M. Tegeder, D. Rentsch, Wolfgang Koch, W. Frommer (2002)
Conservation of amino acid transporters in fungi, plants and animals.Trends in biochemical sciences, 27 3
J.F. Harper, L. Manney, M.R. Sussman (1994)
The 1plasma membrane H+ ATPase gene family in Arabidopsis: Genomic sequence of AHA10 which is expressed primarily in developing seedsPlant J., 29
T.B. Kinraide, B. Etherton (1980)
Electrical evidence for different mechanisms of uptake for basic, neutral, and acidic amino acids in oat coleoptilesPlant J., 29
D. Loo, T. Zeuthen, G. Chandy, Ernest Wright (1996)
Cotransport of water by the Na+/glucose cotransporter.Proceedings of the National Academy of Sciences of the United States of America, 93 23
K. Boorer, W. Frommer, D. Bush, M. Kreman, D. Loo, E. Wright (1996)
Kinetics and Specificity of a H/Amino Acid Transporter from Arabidopsis thaliana(*)The Journal of Biological Chemistry, 271
M. Sugawara, T. Nakanishi, Y. Fei, Wei-qun Huang, M. Ganapathy, F. Leibach, V. Ganapathy (2000)
Cloning of an Amino Acid Transporter with Functional Characteristics and Tissue Expression Pattern Identical to That of System A*The Journal of Biological Chemistry, 275
Z.C. Li, D.R. Bush (1992)
Structural determinants in substrate recognition by proton‐amino acid symporters in plasma membrane vesicles isolated from sugar beet leavesJ. Exp. Bot., 29
H. Winter, D. Robinson, H. Heldt (1993)
Subcellular volumes and metabolite concentrations in barley leavesPlanta, 191
M. Minet, M. Dufour, F. Lacroute (1992)
Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs.The Plant journal : for cell and molecular biology, 2 3
Zhen-Chang Li, Daniel Bush (1991)
DeltapH-Dependent Amino Acid Transport into Plasma Membrane Vesicles Isolated from Sugar Beet (Beta vulgaris L.) Leaves: II. Evidence for Multiple Aliphatic, Neutral Amino Acid Symports.Plant physiology, 96 4
R. Dohmen, A. Strasser, Cornelia Höner, C. Hollenberg (1991)
An efficient transformation procedure enabling long‐term storage of competent cells of various yeast generaYeast, 7
J. Jauniaux, M. Grenson (1990)
GAP1, the general amino acid permease gene of Saccharomyces cerevisiae. Nucleotide sequence, protein similarity with the other bakers yeast amino acid permeases, and nitrogen catabolite repression.European journal of biochemistry, 190 1
Z.C. Li, D.R. Bush (1991)
ΔpH‐Dependent amino acid transport into plasma membrane vesicles isolated form sugar beet leaves. II. Evidence for multiple aliphatic, neutral amino acid symportersArch. Biochem. Biophys., 29
Wolf Frommer, S. Hummel, Michael Unseld, Olaf Ninnemann (1995)
Seed and vascular expression of a high-affinity transporter for cationic amino acids in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America, 92 26
H. Pfanz, K. Dietz (1987)
A Fluorescence Method for the Determination of the Apoplastic Proton Concentration in Intact Leaf tissuesJournal of Plant Physiology, 129
Li Chen, Daniel Bush (1997)
LHT1, A Lysine- and Histidine-Specific Amino Acid Transporter in Arabidopsis, 115
H.M. Lam, K. Coschigano, C. Schultz, R. Melo‐Oliveira, G. Tjaden, I. Oliveira, N. Ngai, M.H. Hsieh, G. Coruzzi (1995)
Use of Arabidopsis mutants and genes to study amide amino acid biosynthesisBot. Acta, 29
R. Reimer, F. Chaudhry, A. Gray, R. Edwards (2000)
Amino acid transport system A resembles system N in sequence but differs in mechanism.Proceedings of the National Academy of Sciences of the United States of America, 97 14
Doris Rentsch, B. Hirner, E. Schmelzer, Wolf Frommer (1996)
Salt stress-induced proline transporters and salt stress-repressed broad specificity amino acid permeases identified by suppression of a yeast amino acid permease-targeting mutant.The Plant cell, 8
J. Pate, P. Sharkey, O. Lewis (2004)
Xylem to phloem transfer of solutes in fruiting shoots of legumes, studied by a phloem bleeding techniquePlanta, 122
R.J. Reimer, F.A. Chaudhry, A.T. Gray, R.H. Edwards (2000)
Amino acid transport System A resembles System N in sequence but differs in mechanismPlant Cell, 29
W. Fischer, B. André, D. Rentsch, Sylvia Krolkiewicz, M. Tegeder, K. Breitkreuz, W. Frommer (1998)
Amino acid transport in plantsTrends in Plant Science, 3
W.N. Fischer, è.B. Andr, D. Rentsch, S. Krolkiewicz, M. Tegeder, K. Breitkreuz, W.B. Frommer (1998)
Plant amino acid transportTrends Plant Sci., 29
K. Leidreiter, A. Kruse, D. Heineke, David Robinson, H. Heldt (1995)
Subcellular Volumes and Metabolite Concentrations in Potato (Solanum tuberosum cv. Désirée) Leaves1, 108
Amides and acidic amino acids represent the major long distance transport forms of organic nitrogen. Six amino acid permeases (AAPs) from Arabidopsis mediating transport of a wide spectrum of amino acids were isolated. AAPs are distantly related to plasma membrane amino acid transport systems N and A and to vesicular transporters such as VGAT from mammals. A detailed comparison of the properties by electrophysiology after heterologous expression in Xenopus oocytes shows that, although capable of recognizing and transporting a wide spectrum of amino acids, individual AAPs differ with respect to specificity. Apparent substrate affinities are influenced by structure and net charge and vary by three orders of magnitude. AAPs mediate cotransport of neutral amino acids with one proton. Uncharged forms of acidic and basic amino acids are cotransported with one proton. Since all AAPs are differentially expressed, different tissues may be supplied with a different spectrum of amino acids. AAP3 and AAP5 are the only transporters mediating efficient transport of the basic amino acids. In vivo competition shows that the capability to transport basic amino acids in planta might be overruled by excess amides and acidic amino acids in the apoplasm. With the exception of AAP6, AAPs do not recognize aspartate; only AAP6 has an affinity for aspartate in the physiologically relevant range. This property is due to an overall higher affinity of AAP6 for neutral and acidic amino acids. Thus AAP6 may serve a different role either in cooperating with the lower affinity systems to acquire amino acids in the low concentration range, as a system responsible for aspartate transport or as an uptake system from the xylem. In agreement, a yeast mutant deficient in acidic amino acid uptake at low aspartate concentrations was complemented only by AAP6. Taken together, the AAPs transport neutral, acidic and cationic amino acids, including the major transport forms, i.e. glutamine, asparagine and glutamate. Increasing proton concentrations strongly activate transport of amino acids. Thus the actual apoplasmic concentration of amino acids and the pH will determine what is transported in vivo, i.e. major amino acids such as glutamine, asparagine, and glutamate will be mobilized preferentially.
The Plant Journal – Wiley
Published: Mar 1, 2002
Keywords: ; ;
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