Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Substances with estrogenic activity in effluents of sewage treatment plants in southwestern Germany. 2. Biological analysis

Substances with estrogenic activity in effluents of sewage treatment plants in southwestern... The proliferation test with human estrogen receptor‐positive MCF‐7 breast cancer cells (E‐Screen assay) was applied for quantitative determination of total estrogenic activity in 24‐h composite effluent samples from 16 municipal and two industrial sewage treatment plants (STPs) in the state of Baden‐Württemberg, southwestern Germany. The estrogenic efficacy relative to the positive control, 17β‐estradiol, was between 26 and 74% (median, 48%) for the 16 municipal STPs. Estradiol equivalent concentrations (EEQs) were between 0.2 and 7.8 ng/L (median, 1.6 ng/L) and, thereby, were lower than those found in a pilot study, which revealed EEQs of greater than 10 ng/L in the effluents of two other STPs. The EEQs in 14 of the 16 effluent samples were very similar (0.9–3.3 ng/L), indicating a rather constant input of estrogenic substances via STPs into rivers. Additional activated charcoal filtration turned out to be very efficient in further eliminating estrogenic activity from effluents. The EEQs of the E‐Screen assay and those calculated from the results of extensive chemical analysis using the estradiol equivalency factors determined for 13 natural and synthetic estrogenic substances were comparable for most of the effluent samples. 17β‐Estradiol, 17α‐ethinylestradiol, and, to a lesser extent, estrone contributed to 90% or more of the EEQ value. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Environmental Toxicology & Chemistry Wiley

Substances with estrogenic activity in effluents of sewage treatment plants in southwestern Germany. 2. Biological analysis

Download PDF
10 pages

Loading next page...
 
/lp/wiley/substances-with-estrogenic-activity-in-effluents-of-sewage-treatment-TJJdgeWbHT

References (58)

Publisher
Wiley
Copyright
Copyright © 2001 SETAC
ISSN
0730-7268
eISSN
1552-8618
DOI
10.1002/etc.5620201002
Publisher site
See Article on Publisher Site

Abstract

The proliferation test with human estrogen receptor‐positive MCF‐7 breast cancer cells (E‐Screen assay) was applied for quantitative determination of total estrogenic activity in 24‐h composite effluent samples from 16 municipal and two industrial sewage treatment plants (STPs) in the state of Baden‐Württemberg, southwestern Germany. The estrogenic efficacy relative to the positive control, 17β‐estradiol, was between 26 and 74% (median, 48%) for the 16 municipal STPs. Estradiol equivalent concentrations (EEQs) were between 0.2 and 7.8 ng/L (median, 1.6 ng/L) and, thereby, were lower than those found in a pilot study, which revealed EEQs of greater than 10 ng/L in the effluents of two other STPs. The EEQs in 14 of the 16 effluent samples were very similar (0.9–3.3 ng/L), indicating a rather constant input of estrogenic substances via STPs into rivers. Additional activated charcoal filtration turned out to be very efficient in further eliminating estrogenic activity from effluents. The EEQs of the E‐Screen assay and those calculated from the results of extensive chemical analysis using the estradiol equivalency factors determined for 13 natural and synthetic estrogenic substances were comparable for most of the effluent samples. 17β‐Estradiol, 17α‐ethinylestradiol, and, to a lesser extent, estrone contributed to 90% or more of the EEQ value.

Journal

Environmental Toxicology & Chemistry – Wiley

Published: Oct 1, 2001

There are no references for this article.