Access the full text.
Sign up today, get DeepDyve free for 14 days.
J. Donaldson, R. Klausner (1994)
ARF: a key regulatory switch in membrane traffic and organelle structure.Current opinion in cell biology, 6 4
C. Turner, K. Burridge (1991)
Transmembrane molecular assemblies in cell-extracellular matrix interactions.Current opinion in cell biology, 3 5
H. Radhakrishna, J. Donaldson (1997)
ADP-Ribosylation Factor 6 Regulates a Novel Plasma Membrane Recycling PathwayThe Journal of Cell Biology, 139
E. Clark, J. Brugge (1995)
Integrins and signal transduction pathways: the road taken.Science, 268 5208
Christopher Turner, John Miller (1994)
Primary sequence of paxillin contains putative SH2 and SH3 domain binding motifs and multiple LIM domains: identification of a vinculin and pp125Fak-binding region.Journal of cell science, 107 ( Pt 6)
M. Schaller, J. Parsons (1995)
pp125FAK-dependent tyrosine phosphorylation of paxillin creates a high-affinity binding site for CrkMolecular and Cellular Biology, 15
Akiko Kondo, Akiko Kondo, S. Hashimoto, H. Yano, K. Nagayama, Y. Mazaki, H. Sabe, H. Sabe (2000)
A new paxillin-binding protein, PAG3/Papalpha/KIAA0400, bearing an ADP-ribosylation factor GTPase-activating protein activity, is involved in paxillin recruitment to focal adhesions and cell migration.Molecular biology of the cell, 11 4
C. Turner, J. Glenney, K. Burridge (1990)
Paxillin: a new vinculin-binding protein present in focal adhesionsThe Journal of Cell Biology, 111
Michael Brown, J. Perrotta, C. Turner (1996)
Identification of LIM3 as the principal determinant of paxillin focal adhesion localization and characterization of a novel motif on paxillin directing vinculin and focal adhesion kinase bindingThe Journal of Cell Biology, 135
A. Hall (1998)
Rho GTPases and the actin cytoskeleton.Science, 279 5350
A. Cesare, Simona Paris, C. Albertinazzi, Simona Dariozzi, J. Andersen, M. Mann, R. Longhi, I. Curtis (2000)
p95-APP1 links membrane transport to Rac-mediated reorganization of actinNature Cell Biology, 2
J. Côté, C. Turner, M. Tremblay (1999)
Intact LIM 3 and LIM 4 Domains of Paxillin Are Required for the Association to a Novel Polyproline Region (Pro 2) of Protein-Tyrosine Phosphatase-PEST*The Journal of Biological Chemistry, 274
J. Castresana, M. Saraste (1995)
Does Vav bind to F‐actin through a CH domain?FEBS Letters, 374
Yu Shen, G. Schneider, J. Cloutier, A. Veillette, M. Schaller (1998)
Direct Association of Protein-tyrosine Phosphatase PTP-PEST with Paxillin*The Journal of Biological Chemistry, 273
S. Bagrodia, Stephen Taylor, K. Jordon, L. Aelst, R. Cerione (1998)
A Novel Regulator of p21-activated Kinases*The Journal of Biological Chemistry, 273
C. Wood, C. Turner, P. Jackson, D. Critchley (1994)
Characterisation of the paxillin-binding site and the C-terminal focal adhesion targeting sequence in vinculin.Journal of cell science, 107 ( Pt 2)
Jia Song, Z. Khachikian, H. Radhakrishna, J. Donaldson (1998)
Localization of endogenous ARF6 to sites of cortical actin rearrangement and involvement of ARF6 in cell spreading.Journal of cell science, 111 ( Pt 15)
Shouchun Liu, Sheila Thomas, D. Woodside, D. Rose, W. Kiosses, M. Pfaff, M. Ginsberg (1999)
Binding of paxillin to α4 integrins modifies integrin-dependent biological responsesNature, 402
F. Corpet (1988)
Multiple sequence alignment with hierarchical clustering.Nucleic acids research, 16 22
J. Luis, Rodrfguez Fermtndez, B. Geiger, D. Salomon, I. Sabanay, Margot Z611er, Avri 'ev (1992)
Suppression of tumorigenicity in transformed cells after transfection with vinculin cDNAThe Journal of Cell Biology, 119
S. Frank, Jessica Hatfield, J. Casanova (1998)
Remodeling of the actin cytoskeleton is coordinately regulated by protein kinase C and the ADP-ribosylation factor nucleotide exchange factor ARNO.Molecular biology of the cell, 9 11
E. Clark, W. King, J. Brugge, M. Symons, R. Hynes (1998)
Integrin-mediated Signals Regulated by Members of the Rho Family of GTPasesThe Journal of Cell Biology, 142
Histaka Sabe, Akiko Hata, Masato Okada, Hachiro Nakagawa, Hidesaburo Hanafusa (1994)
Analysis of the binding of the Src homology 2 domain of Csk to tyrosine-phosphorylated proteins in the suppression and mitotic activation of c-Src.Proceedings of the National Academy of Sciences of the United States of America, 91 9
A. Lupas, M. Dyke, J. Stock (1991)
Predicting coiled coils from protein sequencesScience, 252
Jörg Hagmann, Marianne Grob, A. Welman, G. Willigen, M. Burger (1998)
Recruitment of the LIM protein hic-5 to focal contacts of human platelets.Journal of cell science, 111 ( Pt 15)
J. Norman, Dylan Jones, S. Barry, M. Holt, S. Cockcroft, D. Critchley (1998)
ARF1 Mediates Paxillin Recruitment to Focal Adhesions and Potentiates Rho-stimulated Stress Fiber Formation in Intact and Permeabilized Swiss 3T3 FibroblastsThe Journal of Cell Biology, 143
C. Turner (1998)
Molecules in focus PaxillinThe International Journal of Biochemistry & Cell Biology, 30
R. Resnick, Stephen Taylor, Qiong Lin, D. Shalloway (1997)
Phosphorylation of the Src substrate Sam68 by Cdc2 during mitosisOncogene, 15
J. Tidball, Theresa, Keith Burridge (1986)
Talin at myotendinous junctionsThe Journal of Cell Biology, 103
K. Burridge, M. Chrzanowska-Wodnicka (1996)
Focal adhesions, contractility, and signaling.Annual review of cell and developmental biology, 12
C. Nobes, A. Hall (1995)
Rho, Rac, and Cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodiaCell, 81
K. Tachibana, Toshiyasu Sato, N. D’Avirro, C. Morimoto (1995)
Direct association of pp125FAK with paxillin, the focal adhesion- targeting mechanism of pp125FAKThe Journal of Experimental Medicine, 182
C. Turner (2020)
Paxillin.The international journal of biochemistry & cell biology, 30 9
S. Pol, Michael Brown, C. Turner (1998)
Association of Bovine Papillomavirus Type 1 E6 oncoprotein with the focal adhesion protein paxillin through a conserved protein interaction motifOncogene, 16
L. Aelst, C. D’Souza-Schorey (1997)
Rho GTPases and signaling networks.Genes & development, 11 18
R.Hugh Daniels, G. Bokoch (1999)
p21-activated protein kinase: a crucial component of morphological signaling?Trends in biochemical sciences, 24 9
R. Premont, A. Claing, N. Vitale, S. Perry, R. Lefkowitz (2000)
The GIT Family of ADP-ribosylation Factor GTPase-activating ProteinsThe Journal of Biological Chemistry, 275
C. Turner, N. Kramarcy, R. Sealock, K. Burridge (1991)
Localization of paxillin, a focal adhesion protein, to smooth muscle dense plaques, and the myotendinous and neuromuscular junctions of skeletal muscle.Experimental cell research, 192 2
R. Salgia, S. Avraham, E. Pisick, Jian-Liang Li, S. Raja, E. Greenfield, M. Sattler, H. Avraham, J. Griffin (1996)
The Related Adhesion Focal Tyrosine Kinase Forms a Complex with Paxillin in Hematopoietic Cells*The Journal of Biological Chemistry, 271
Michael Brown, J. Perrotta, C. Turner (1998)
Serine and threonine phosphorylation of the paxillin LIM domains regulates paxillin focal adhesion localization and cell adhesion to fibronectin.Molecular biology of the cell, 9 7
B. Kay, M. Williamson, M. Sudol (2000)
The importance of being proline: the interaction of proline‐rich motifs in signaling proteins with their cognate domainsThe FASEB Journal, 14
C. Turner, Michael Brown, J. Perrotta, M. Riedy, S. Nikolopoulos, A. McDonald, S. Bagrodia, S. Thomas, P. Leventhal (1999)
Paxillin LD4 Motif Binds PAK and PIX through a Novel 95-kD Ankyrin Repeat, ARF–GAP Protein: A Role in Cytoskeletal RemodelingThe Journal of Cell Biology, 145
Giancotti (1999)
Integrin signalingScience, 285
I. Correia, D. Chu, Y. Chou, R. Goldman, P. Matsudaira (1999)
Integrating the Actin and Vimentin CytoskeletonsThe Journal of Cell Biology, 146
L. Price, J. Leng, M. Schwartz, G. Bokoch (1998)
Activation of Rac and Cdc42 by integrins mediates cell spreading.Molecular biology of the cell, 9 7
Xiao Tong, Ravi Salgia, Jian-Liang Li, James Griffin, P. Howley (1997)
The Bovine Papillomavirus E6 Protein Binds to the LD Motif Repeats of Paxillin and Blocks Its Interaction with Vinculin and the Focal Adhesion Kinase*The Journal of Biological Chemistry, 272
S. Bagrodia, R. Cerione (1999)
Pak to the future.Trends in cell biology, 9 9
B. Jockusch, P. Bubeck, K. Giehl, M. Kroemker, Jutta Moschner, Martin Rothkegel, M. Rüdiger, K. Schlüter, Gesa Stanke, J. Winkler (1995)
The molecular architecture of focal adhesions.Annual review of cell and developmental biology, 11
K. Carugo, S. Bañuelos, M. Saraste (1997)
Crystal structure of a calponin homology domainNature Structural Biology, 4
Michael Brown, Michael Curtis, C. Turner (1998)
Paxillin LD motifs may define a new family of protein recognition domainsNature Structural &Molecular Biology, 5
S. Craig, R. Johnson (1996)
Assembly of focal adhesions: progress, paradigms, and portents.Current opinion in cell biology, 8 1
Sonia Bañuelos, Matti Saraste, K. Carugo (1998)
Structural comparisons of calponin homology domains: implications for actin binding.Structure, 6 11
J. Donaldson, C. Jackson (2000)
Regulators and effectors of the ARF GTPases.Current opinion in cell biology, 12 4
S. Goldsmith, N. Pokala, W. Shen, A. Fedorov, P. Matsudaira, S. Almo (1997)
The structure of an actin-crosslinking domain from human fimbrinNature Structural Biology, 4
J. Hildebrand, J. Taylor, J. Parsons (1996)
An SH3 domain-containing GTPase-activating protein for Rho and Cdc42 associates with focal adhesion kinaseMolecular and Cellular Biology, 16
E. Manser, T. Loo, C. Koh, Zhou-shen Zhao, Xiang-qun Chen, L. Tan, I. Tan, T. Leung, L. Lim (1998)
PAK kinases are directly coupled to the PIX family of nucleotide exchange factors.Molecular cell, 1 2
Theresia Stradal, W. Kranewitter, S. Winder, M. Gimona (1998)
CH domains revisitedFEBS Letters, 431
Paxillin is a focal adhesion adapter protein involved in the integration of growth factor– and adhesion-mediated signal transduction pathways. Paxillin LD motifs have been demonstrated to bind to several proteins associated with remodeling of the actin cytoskeleton including the focal adhesion kinase, vinculin, and a complex of proteins comprising p95PKL, PIX, and PAK (Turner, C.E., M.C. Brown, J.A. Perrotta, M.C. Riedy, S.N. Nikolopoulos, A.R. McDonald, S. Bagrodia, S. Thomas, and P.S. Leventhal. 1999. J. Cell Biol . 145:851–863). In this study, we report the cloning and initial characterization of a new paxillin LD motif–binding protein, actopaxin. Analysis of the deduced amino acid sequence of actopaxin reveals a 42-kD protein with two calponin homology domains and a paxillin-binding subdomain (PBS). Western blotting identifies actopaxin as a widely expressed protein. Actopaxin binds directly to both F-actin and paxillin LD1 and LD4 motifs. It exhibits robust focal adhesion localization in several cultured cell types but is not found along the length of the associated actin-rich stress fibers. Similar to paxillin, it is absent from actin-rich cell–cell adherens junctions. Also, actopaxin colocalizes with paxillin to rudimentary focal complexes at the leading edge of migrating cells. An actopaxin PBS mutant incapable of binding paxillin in vitro cannot target to focal adhesions when expressed in fibroblasts. In addition, ectopic expression of the PBS mutant and/or the COOH terminus of actopaxin in HeLa cells resulted in substantial reduction in adhesion to collagen. Together, these results suggest an important role for actopaxin in integrin-dependent remodeling of the actin cytoskeleton during cell motility and cell adhesion. actin-binding protein CH domains focal adhesions paxillin hic-5 Footnotes Abbreviations used in this paper: aa, amino acid(s); Arf, ADP-ribosylation factor; CH, calponin homology; FAK, focal adhesion kinase; GST, glutathione S -transferase; HISM, human intestinal smooth muscle; PBS, paxillin-binding subdomain; RT, reverse transcriptase. Submitted: 11 May 2000 Revision requested 31 October 2000 Accepted: 1 November 2000
The Journal of Cell Biology – Rockefeller University Press
Published: Dec 25, 2000
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.