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In vivo comparison of xenoestrogens using rainbow trout vitellogenin induction as a screening system

In vivo comparison of xenoestrogens using rainbow trout vitellogenin induction as a screening system Vitellogenin induction in rainbow trout after intraperitoneal injection of test compounds was investigated. Vitellogenin quantification was performed by a direct sandwich enzyme‐linked immunosorbant assay (ELISA) based on polyclonal, affinity purified antibodies. The system was used as a short‐term in vivo screen for estrogenicity and as a tool for establishing the priority of compounds to be tested by water exposure of fish. Representatives from the group of xenoestrogens, bisphenols, phthalates, and pesticides were tested. The relative potency order bisphenol A > bisphenol A dimethacrylate, o,p′‐DDT > butylbenzylphthalate was established, while no significant vitellogenin response was seen with dibutylphthalate, tetrabromobisphenol A, and methoxychlor. Of these, tetrabromobisphenol A has not previously been tested for estrogenicity in vivo. Earlier findings of diminished estrogenic activity of ortho‐substituted compounds was extended to bisphenols since ortho‐bromination of bisphenol A abolished the activity of the parent molecule. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Environmental Toxicology & Chemistry Wiley

In vivo comparison of xenoestrogens using rainbow trout vitellogenin induction as a screening system

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References (46)

Publisher
Wiley
Copyright
Copyright © 2000 SETAC
ISSN
0730-7268
eISSN
1552-8618
DOI
10.1002/etc.5620190721
Publisher site
See Article on Publisher Site

Abstract

Vitellogenin induction in rainbow trout after intraperitoneal injection of test compounds was investigated. Vitellogenin quantification was performed by a direct sandwich enzyme‐linked immunosorbant assay (ELISA) based on polyclonal, affinity purified antibodies. The system was used as a short‐term in vivo screen for estrogenicity and as a tool for establishing the priority of compounds to be tested by water exposure of fish. Representatives from the group of xenoestrogens, bisphenols, phthalates, and pesticides were tested. The relative potency order bisphenol A > bisphenol A dimethacrylate, o,p′‐DDT > butylbenzylphthalate was established, while no significant vitellogenin response was seen with dibutylphthalate, tetrabromobisphenol A, and methoxychlor. Of these, tetrabromobisphenol A has not previously been tested for estrogenicity in vivo. Earlier findings of diminished estrogenic activity of ortho‐substituted compounds was extended to bisphenols since ortho‐bromination of bisphenol A abolished the activity of the parent molecule.

Journal

Environmental Toxicology & ChemistryWiley

Published: Jul 1, 2000

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