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P. Krieg, D. Melton (1984)
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.Nucleic acids research, 12 18
T. Ford-Holevinski, B. Agranoff, N. Radin (1983)
An inexpensive, microcomputer-based, video densitometer for quantitating thin-layer chromatographic spots.Analytical biochemistry, 132 1
Itoh Itoh, Slemmon Slemmon, Hawke Hawke, Williamson Williamson, Morita Morita, Itakura Itakura, Roberts Roberts, Shively Shively, Crawford Crawford, Salvaterra Salvaterra (1986)
Cloning of Drosophila choline acetyltransferase cDNAProc Natl Acad Sci USA, 83
P. Cohen (1988)
Review Lecture: Protein phosphorylation and hormone actionProceedings of the Royal Society of London. Series B. Biological Sciences, 234
White White, Cavallito Cavallito (1970)
Choline acetyltransferaseEnzyme mechanism and mode of inhibition by a styrylpyridine analog. Biochim Biophys Acata, 206
Maria Melis, G. Demopulos, V. NAJFELDt, Jun-wu Zhang, M. Brice, Thalia PAPAYANNOPOULOUt, G. Stamatoyannopoulos (1987)
A chromosome 11-linked determinant controls fetal globin expression and the fetal-to-adult globin switch.Proceedings of the National Academy of Sciences of the United States of America, 84 22
K. Fukada (1985)
Purification and partial characterization of a cholinergic neuronal differentiation factor.Proceedings of the National Academy of Sciences of the United States of America, 82 24
Dietz Dietz, Salvaterra Salvaterra (1980)
Purification and peptide mapping of rat brain choline acetyltransferaseJ Biol Chem, 255
H. Pelham, R. Jackson (1976)
An efficient mRNA-dependent translation system from reticulocyte lysates.European journal of biochemistry, 67 1
U. Gubler, B. Hoffman (1983)
A simple and very efficient method for generating cDNA libraries.Gene, 25 2-3
Cohen Cohen (1988)
Protein phosphorylation and hormone regulationProc R Soc Lond, 234
S. Berrard, Alexis Brice, Friedrich Lottspeich, Axel Braun, Y. Barde, Jacques Mallet (1987)
cDNA cloning and complete sequence of porcine choline acetyltransferase: in vitro translation of the corresponding RNA yields an active protein.Proceedings of the National Academy of Sciences of the United States of America, 84 24
Hawrot Hawrot, Patterson Patterson (1979)
Long‐term culture of dissociated sympathetic neuronsMethods Enzymol, 53
D. Melton, P. Krieg, M. Rebagliati, T. Maniatis, K. Zinn, Michael Green (1984)
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.Nucleic acids research, 12 18
F. Sanger, S. Nicklen, A. Coulson (1977)
DNA sequencing with chain-terminating inhibitors.Proceedings of the National Academy of Sciences of the United States of America, 74 12
Kozak Kozak (1984)
Compilation and analysis of sequences upstream from the translational start site in eucaryotic mRNAsNucleic Acids Res, 12
Swerts Swerts, Weber Weber (1984)
Regulation of enzymes responsible for neurotransmitter synthesis and degradation in cultured rat sympathetic neurons. III. Effects of sodium butyrateDev Biol, 106
I. Black, J. Adler, C. Dreyfus, G. Jonakait, D. Katz, E. LaGamma, K. Markey (1984)
Neurotransmitter plasticity at the molecular level.Science, 225 4668
Feinberg Feinberg, Vogelstein Vogelstein (1983)
A technique for radiolabelling DNA restriction fragment to high specific activityAnal Biochem, 132
Faucon Biguet Faucon Biguet, Buda Buda, Lamouroux Lamouroux, Samolyk Samolyk, Mallet Mallet (1986)
Time course of the changes of TH mRNA in rat brain and adrenal medulla after a single injection of reserpineEMBO J, 5
G. Blankenhorn (1976)
Nicotinamide-dependent one-electron and two-electron (flavin) oxidoreduction: thermodynamics, kinetics, and mechanism.European journal of biochemistry, 67 1
Swerts Swerts, Le Van Thaï Le Van Thaï, Vigny Vigny, Weber Weber (1983)
Regulation of enzymes responsible for neurotransmitter synthesis and degradation in cultured rat sympathetic neurons. I. Effects of muscle conditioned mediumDev Biol, 100
A. Feinberg, B. Vogelstein (1983)
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.Analytical biochemistry, 132 1
B. Raynaud, N. Faucon‐Biguet, S. Vidal, J. Mallet, M. Weber (1987)
The use of a tyrosine-hydroxylase cDNA probe to study the neurotransmitter plasticity of rat sympathetic neurons in culture.Developmental biology, 119 2
R. Zigmond (1985)
Biochemical consequences of synaptic stimulation: The regulation of tyrosine hydroxylase activity by multiple transmittersTrends in Neurosciences, 8
F. Fonnum (1975)
A rapid radiochemical method for the determination of choline acetyltransferaseJournal of Neurochemistry, 24
A. Lamouroux, N. Biguet, D. Samolyk, A. Privat, J. Salomon, J. Pujol, J. Mallet (1982)
Identification of cDNA clones coding for rat tyrosine hydroxylase antigen.Proceedings of the National Academy of Sciences of the United States of America, 79 12
Potter Potter, Landis Landis, Matsumoto Matsumoto, Furshpan Furshpan (1986)
Synaptic functions in rat sympathetic neurons in microcultures. II. Adrenergic/cholinergic dual status and plasticityJ Neurosci, 6
B. Grima, A. Lamouroux, Ois Blanot, N. Biguet, Jacques Mallet (1985)
Complete coding sequence of rat tyrosine hydroxylase mRNA.Proceedings of the National Academy of Sciences of the United States of America, 82 2
Björklund Björklund, Hökfelt Hökfelt, Goldstein Goldstein, Terenius Terenius, Olson Olson (1985)
Appearence of the noradrenergic markers tyrosine hydroxylase and neuropeptide Y in cholinergic nerves of the iris following sympathectomyJ Neurosci, 5
P. Deininger (1983)
Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis.Analytical biochemistry, 129 1
R. Saiki, S. Scharf, F. Faloona, K. Mullis, G. Horn, H. Erlich, N. Arnheim (1985)
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.Science, 230 4732
S. Berrard, N. Biguet, Danielle Gregoire, F. Blanot, Julian Smith, J. Mallet (1986)
Synthesis of catalytically active choline acetyltransferase in Xenopus oocytes injected with messenger RNA from rat central nervous systemNeuroscience Letters, 72
P. Lomedico, G. Saunders (1976)
Preparation of pancreatic mRNA: cell-free translation of an insulin-immunoreactive polypeptide.Nucleic acids research, 3 2
A cDNA clone encoding the complete sequence of an active, rat choline acetyltransferase (ChoAcTase; acetyl‐CoA:choline O‐acetyltransferase, EC 2.3.1.6) has been isolated. Analysis of the deduced amino acid sequence reveals 85% and 31% identity with the porcine and Drosophila melanogaster enzymes, respectively. To further elucidate the molecular basis of neurotransmitter‐related phenotypic plasticity, the expression of ChoAcTase mRNA was compared with that of tyrosine hydroxylase (TH; tyrosine 3‐monooxygenase, L‐tyrosine, tetrahydropteridine: oxygen oxidoreductase (3‐hydroxylating), EC 1.14.16.2), in neurons from superior cervical ganglia grown in the following conditions: (1) normal medium, (2) high K+ medium, and (3) normal medium supplemented with 50% muscle‐conditioned medium (CM). TH mRNA was expressed in all three media; its level rose in high K+ and decreased strikingly in the presence of CM. ChoAcTase mRNA could be visualized in CM, but fell to undetectable levels in normal and high K+ media. These results suggest that translational or post‐translational mechanisms do not play a major role for the modulation of neurotransmitter‐associated phenotype.
Journal of Neuroscience Research – Wiley
Published: Jul 1, 1989
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