Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Multiple‐locus variable number tandem repeats analysis for genetic fingerprinting of pathogenic bacteria

Multiple‐locus variable number tandem repeats analysis for genetic fingerprinting of pathogenic... DNA fingerprinting has attracted considerable interest as means for identifying, tracing and preventing the dissemination of infectious agents. Various methods have been developed for typing of pathogenic bacteria, which differ in discriminative power, reproducibility and ease of interpretation. During recent years a typing method, which uses the information provided by whole genome sequencing of bacterial species, has gained increased attention. Short sequence repeat (SSR) motifs are known to undergo frequent variation in the number of repeated units through cellular mechanisms most commonly active during chromosome replication. A class of SSRs, named variable number of tandem repeats (VNTRs), has proven to be a suitable target for assessing genetic polymorphisms within bacterial species. This review attempts to give an overview of bacterial agents where VNTR‐based typing, or multiple‐locus variant‐repeat analysis (MLVA) has been developed for typing purposes, together with addressing advantages and drawbacks associated with the use of tandem repeated DNA motifs as targets for bacterial typing and identification. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Electrophoresis Wiley

Multiple‐locus variable number tandem repeats analysis for genetic fingerprinting of pathogenic bacteria

Lindstedt, Bjørn‐Arne
Electrophoresis , Volume 26 (13) – Jul 1, 2005
Download PDF
16 pages

Loading next page...
 
/lp/wiley/multiple-locus-variable-number-tandem-repeats-analysis-for-genetic-QvVls6n1LV

References (150)

Publisher
Wiley
Copyright
"Copyright © 2005 Wiley Subscription Services, Inc., A Wiley Company"
ISSN
0173-0835
eISSN
1522-2683
DOI
10.1002/elps.200500096
pmid
15937984
Publisher site
See Article on Publisher Site

Abstract

DNA fingerprinting has attracted considerable interest as means for identifying, tracing and preventing the dissemination of infectious agents. Various methods have been developed for typing of pathogenic bacteria, which differ in discriminative power, reproducibility and ease of interpretation. During recent years a typing method, which uses the information provided by whole genome sequencing of bacterial species, has gained increased attention. Short sequence repeat (SSR) motifs are known to undergo frequent variation in the number of repeated units through cellular mechanisms most commonly active during chromosome replication. A class of SSRs, named variable number of tandem repeats (VNTRs), has proven to be a suitable target for assessing genetic polymorphisms within bacterial species. This review attempts to give an overview of bacterial agents where VNTR‐based typing, or multiple‐locus variant‐repeat analysis (MLVA) has been developed for typing purposes, together with addressing advantages and drawbacks associated with the use of tandem repeated DNA motifs as targets for bacterial typing and identification.

Journal

Electrophoresis – Wiley

Published: Jul 1, 2005

Keywords: ; ; ;

There are no references for this article.