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Porcine malignant hyperthermia carrier detection and chromosomal assignment using a linked probe

Porcine malignant hyperthermia carrier detection and chromosomal assignment using a linked probe Summary. In pigs, the gene for glucosephosphate isomerase (GPI) is linked to the halothane (HAL) gene which is responsible for malignant hyperthermia (MH). A single copy DNA probe, designated GPI8R, has been isolated from a pig genomic library using a porcine GPI cDNA probe. This probe detects, as was the case for the cDNA probe, a five allele polymorphism in Sac1 andPvuII digested pig DNA. Family studies show that this polymorphism is linked to the HAL locus and hence can be used in carrier detection. In situ hybridization with GPI8R assigned the GPI locus to bands p12‐q22 of chromosome 6. We conclude that the HAL linkage group resides on chromosome 6. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Animal Genetics Wiley

Porcine malignant hyperthermia carrier detection and chromosomal assignment using a linked probe

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References (52)

Publisher
Wiley
Copyright
Copyright © 1988 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0268-9146
eISSN
1365-2052
DOI
10.1111/j.1365-2052.1988.tb00809.x
Publisher site
See Article on Publisher Site

Abstract

Summary. In pigs, the gene for glucosephosphate isomerase (GPI) is linked to the halothane (HAL) gene which is responsible for malignant hyperthermia (MH). A single copy DNA probe, designated GPI8R, has been isolated from a pig genomic library using a porcine GPI cDNA probe. This probe detects, as was the case for the cDNA probe, a five allele polymorphism in Sac1 andPvuII digested pig DNA. Family studies show that this polymorphism is linked to the HAL locus and hence can be used in carrier detection. In situ hybridization with GPI8R assigned the GPI locus to bands p12‐q22 of chromosome 6. We conclude that the HAL linkage group resides on chromosome 6.

Journal

Animal GeneticsWiley

Published: Jun 1, 1988

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