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The two RNA polymerases encoded by the nuclear and the plastid compartments transcribe distinct groups of genes in tobacco plastids

The two RNA polymerases encoded by the nuclear and the plastid compartments transcribe distinct... The plastid genome in photosynthetic higher plants encodes subunits of an Escherichia coli‐like RNA polymerase (PEP) which initiates transcription from E.coli σ70‐type promoters. We have previously established the existence of a second nuclear‐encoded plastid RNA polymerase (NEP) in photosynthetic higher plants. We report here that many plastid genes and operons have at least one promoter each for PEP and NEP (Class II transcription unit). However, a subset of plastid genes, including photosystem I and II genes, are transcribed from PEP promoters only (Class I genes), while in some instances (e.g. accD) genes are transcribed exclusively by NEP (Class III genes). Sequence alignment identified a 10 nucleotide NEP promoter consensus around the transcription initiation site. Distinct NEP and PEP promoters reported here provide a general mechanism for group‐specific gene expression through recognition by the two RNA polymerases. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The EMBO Journal Wiley

The two RNA polymerases encoded by the nuclear and the plastid compartments transcribe distinct groups of genes in tobacco plastids

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References (26)

Publisher
Wiley
Copyright
Copyright © 2013 Wiley Periodicals, Inc
ISSN
0261-4189
eISSN
1460-2075
DOI
10.1093/emboj/16.13.4041
pmid
9233813
Publisher site
See Article on Publisher Site

Abstract

The plastid genome in photosynthetic higher plants encodes subunits of an Escherichia coli‐like RNA polymerase (PEP) which initiates transcription from E.coli σ70‐type promoters. We have previously established the existence of a second nuclear‐encoded plastid RNA polymerase (NEP) in photosynthetic higher plants. We report here that many plastid genes and operons have at least one promoter each for PEP and NEP (Class II transcription unit). However, a subset of plastid genes, including photosystem I and II genes, are transcribed from PEP promoters only (Class I genes), while in some instances (e.g. accD) genes are transcribed exclusively by NEP (Class III genes). Sequence alignment identified a 10 nucleotide NEP promoter consensus around the transcription initiation site. Distinct NEP and PEP promoters reported here provide a general mechanism for group‐specific gene expression through recognition by the two RNA polymerases.

Journal

The EMBO Journal – Wiley

Published: Jan 1, 1997

Keywords: ; ; ; ;

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