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The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications. FRET microscopy; data analysis; cameleons; dimerization; FRET assays Footnotes ↵ * Abbreviations used in this paper: CAM, calmodulin; EGFR, EGF receptor; FRET, fluorescence resonance energy transfer; MP, multiphoton; SBT, spectral bleedthrough. The online version of this article includes supplemental material. Submitted: 24 October 2002 Accepted: 21 January 2003 Revision received 21 January 2003
The Journal of Cell Biology – Rockefeller University Press
Published: Mar 3, 2003
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