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Macrophages at the skeletal tissue–device interface of loosened prosthetic devices express bone‐related genes and their products

Macrophages at the skeletal tissue–device interface of loosened prosthetic devices express... Aseptic loosening of prosthetic arthroplasty is the most common reason for implant failure in adult orthopaedic reconstruction. At the interface of aseptic loosened prostheses, there is an abundance of particle‐activated macrophages and other inflammatory cells. The role of these particle‐laden macrophages in the osteogenic arm of the remodeling skeleton in this pathological condition is poorly understood. Molecular signaling by mesenchymal cells and mononuclear inflammatory cells residing in the interfacial tissues between bone and cement or prosthetic material of aseptically loosened joint prostheses was studied using in situ hybridization and immunohistochemical techniques. We found that a range of collagenous and noncollagenous matrix proteins, including osteopontin, osteocalcin, bone sialoprotein, and type I collagen, were produced in the periprosthetic tissue by foamy macrophages, as well as nearby osteogenic cells. The former accumulated in profusion in the three zones of interfacial tissues: pseudomembranous, fibrous, and osseous. Spindle mesenchymal cells in the fibrous zone failed to express any of the osteogenic mRNAs or proteins sought. The expression of bone‐related genes and proteins by foamy macrophages at the interface of an aseptic loosened prosthesis may contribute to the disturbance of bone remodeling at this site. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 65A: 109–117, 2003 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biomedical Materials Research Part A Wiley

Macrophages at the skeletal tissue–device interface of loosened prosthetic devices express bone‐related genes and their products

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References (32)

Publisher
Wiley
Copyright
"Copyright © 2003 Wiley Subscription Services, Inc., A Wiley Company"
ISSN
1549-3296
eISSN
1552-4965
DOI
10.1002/jbm.a.10441
pmid
12635160
Publisher site
See Article on Publisher Site

Abstract

Aseptic loosening of prosthetic arthroplasty is the most common reason for implant failure in adult orthopaedic reconstruction. At the interface of aseptic loosened prostheses, there is an abundance of particle‐activated macrophages and other inflammatory cells. The role of these particle‐laden macrophages in the osteogenic arm of the remodeling skeleton in this pathological condition is poorly understood. Molecular signaling by mesenchymal cells and mononuclear inflammatory cells residing in the interfacial tissues between bone and cement or prosthetic material of aseptically loosened joint prostheses was studied using in situ hybridization and immunohistochemical techniques. We found that a range of collagenous and noncollagenous matrix proteins, including osteopontin, osteocalcin, bone sialoprotein, and type I collagen, were produced in the periprosthetic tissue by foamy macrophages, as well as nearby osteogenic cells. The former accumulated in profusion in the three zones of interfacial tissues: pseudomembranous, fibrous, and osseous. Spindle mesenchymal cells in the fibrous zone failed to express any of the osteogenic mRNAs or proteins sought. The expression of bone‐related genes and proteins by foamy macrophages at the interface of an aseptic loosened prosthesis may contribute to the disturbance of bone remodeling at this site. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 65A: 109–117, 2003

Journal

Journal of Biomedical Materials Research Part A – Wiley

Published: Jan 1, 2003

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