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Inhibition by various antipsychotic drugs of the G‐protein‐activated inwardly rectifying K + (GIRK) channels expressed in Xenopus oocytes

Inhibition by various antipsychotic drugs of the G‐protein‐activated inwardly rectifying K +... To investigate the effects of various chemical classes of antipsychotic drugs: haloperidol, thioridazine, pimozide and clozapine, on the G‐protein‐activated inwardly rectifying K+ (GIRK) channels, we carried out Xenopus oocyte functional assays with GIRK1 and GIRK2 mRNAs or GIRK1 and GIRK4 mRNAs. In oocytes co‐injected with GIRK1 and GIRK2 mRNAs, application of each of the various antipsychotic drugs immediately caused a reduction of inward currents through the basally active GIRK channels. These responses were not observed in the presence of 3 mM Ba2+, which blocks the GIRK channels. In addition, in uninjected oocytes, none of the drugs tested produced any significant current response. These results indicate that all the antipsychotic drugs tested inhibited the brain‐type GIRK1/2 heteromultimeric channels. Furthermore, similar results were obtained in oocytes co‐injected with GIRK1 and GIRK4 mRNAs, indicating that the antipsychotic drugs also inhibited the cardiac‐type GIRK1/4 heteromultimeric channels. All the drugs tested inhibited, in a concentration‐dependent manner, both types of GIRK channels with varying degrees of potency and effectiveness at micromolar concentrations. Only pimozide caused slight inhibition of these channels at nanomolar concentrations. We conclude that the various antipsychotic drugs acted as inhibitors at the brain‐type and cardiac‐type GIRK channels. Our results suggest that inhibition of both types of GIRK channels by these drugs underlies some of the side effects, in particular seizures and sinus tachycardia, observed in clinical practice. British Journal of Pharmacology (2000) 129, 1716–1722; doi:10.1038/sj.bjp.0703224 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png British Journal of Pharmacology Wiley

Inhibition by various antipsychotic drugs of the G‐protein‐activated inwardly rectifying K + (GIRK) channels expressed in Xenopus oocytes

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References (52)

Publisher
Wiley
Copyright
2000 British Pharmacological Society
ISSN
0007-1188
eISSN
1476-5381
DOI
10.1038/sj.bjp.0703224
pmid
10780978
Publisher site
See Article on Publisher Site

Abstract

To investigate the effects of various chemical classes of antipsychotic drugs: haloperidol, thioridazine, pimozide and clozapine, on the G‐protein‐activated inwardly rectifying K+ (GIRK) channels, we carried out Xenopus oocyte functional assays with GIRK1 and GIRK2 mRNAs or GIRK1 and GIRK4 mRNAs. In oocytes co‐injected with GIRK1 and GIRK2 mRNAs, application of each of the various antipsychotic drugs immediately caused a reduction of inward currents through the basally active GIRK channels. These responses were not observed in the presence of 3 mM Ba2+, which blocks the GIRK channels. In addition, in uninjected oocytes, none of the drugs tested produced any significant current response. These results indicate that all the antipsychotic drugs tested inhibited the brain‐type GIRK1/2 heteromultimeric channels. Furthermore, similar results were obtained in oocytes co‐injected with GIRK1 and GIRK4 mRNAs, indicating that the antipsychotic drugs also inhibited the cardiac‐type GIRK1/4 heteromultimeric channels. All the drugs tested inhibited, in a concentration‐dependent manner, both types of GIRK channels with varying degrees of potency and effectiveness at micromolar concentrations. Only pimozide caused slight inhibition of these channels at nanomolar concentrations. We conclude that the various antipsychotic drugs acted as inhibitors at the brain‐type and cardiac‐type GIRK channels. Our results suggest that inhibition of both types of GIRK channels by these drugs underlies some of the side effects, in particular seizures and sinus tachycardia, observed in clinical practice. British Journal of Pharmacology (2000) 129, 1716–1722; doi:10.1038/sj.bjp.0703224

Journal

British Journal of PharmacologyWiley

Published: Apr 1, 2000

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