Access the full text.
Sign up today, get DeepDyve free for 14 days.
J. Fry, A. Barnason, R. Horsch (1987)
Transformation of Brassica napus with Agrobacterium tumefaciens based vectorsPlant Cell Reports, 6
W. Kysely, J. Myers, P. Lazzeri, G. Collins, H. Jacobsen (1987)
Plant regeneration via somatic embryogenesis in pea (Pisum sativum L.)Plant Cell Reports, 6
R. Deblaere, B. Bytebier, H. Greve, F. Deboeck, J. Schell, M. Montagu, Jan Leemans (1985)
Efficient octopine Ti plasmid-derived vectors for Agrobacterium- mediated gene transfer to plantsNucleic acids research, 13 13
E. Shahin, R. Simpson (1986)
Gene Transfer System for PotatoHortScience
K. Weising, Jeff Schell, Günter Kahl (1988)
Foreign genes in plants: transfer, structure, expression, and applications.Annual review of genetics, 22
D. Evans (1986)
CHAPTER 21 – Case Histories of Genetic Variability in Vitro: Tomato
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual
J. Puonti-Kaerlas, T. Eriksson, P. Engström (1990)
Production of transgenic pea (Pisum sativum L.) plants by Agrobacterium tumefaciens — mediated gene transferTheoretical and Applied Genetics, 80
L. Natali, A. Cavallini (1987)
Regeneration of pea (Pisum sativum L.) plantlets by in vitro culture of immature embryosPlant Breeding, 99
G. Vervliet, M. Holsters, H. Teuchy, M. Montagu, J. Schell (1975)
Characterization of different plaque-forming and defective temperate phages in Agrobacterium.The Journal of general virology, 26 1
G. Gheysen, P. D'Haese, M. Montagu, J. Schell (1985)
DNA Flux Across Genetic Barriers: The Crown Gall Phenomenon, 2
K. De, S. Roy (1985)
Morphogenetic investigation on pea under in vitro conditionsBulletin of the Torrey Botanical Club, 112
R. Ahmed, S. Gupta, P. Ghosh (1987)
The Cytological Status of Plants Regenerated from Shoot‐Meristem Culture of Pisum sativum LPlant Breeding, 98
S. McCormick, Jeanne Niedermeyer, J. Fry, A. Barnason, R. Horsch, R. Fraley (1986)
Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciensPlant Cell Reports, 5
J. Manners (1988)
Transgenic plants of the tropical pasture legume stylosanthes humilisPlant Science, 55
Paul Christou, William Swain, Ning-Sun Yang, D. Mccabe (1989)
Inheritance and expression of foreign genes in transgenic soybean plants.Proceedings of the National Academy of Sciences of the United States of America, 86 19
P. Elzen, J. Townsend, Kathleen Lee, J. Bedbrook (1985)
A chimaeric hygromycin resistance gene as a selectable marker in plant cellsPlant Molecular Biology, 5
L. Pijnacker, K. Walch, M. Ferwerda (1986)
Behaviour of chromosomes in potato leaf tissue cultured in vitro as studied by BrdC-Giemsa labellingTheoretical and Applied Genetics, 72
A. Matthysse, J. Torrey (1967)
Nutritional Requirements for Polyploid Mitoses in Cultured Pea Root SegmentsPhysiologia Plantarum, 20
T. Murashige, F. Skoog (1962)
A revised medium for rapid growth and bio assays with tobacco tissue culturesPhysiologia Plantarum, 15
M. Lulsdorf, H. Rempel, Jennie Jackson, D. Baliski, S. Hobbs (2004)
Optimizing the production of transformed pea (Pisum sativum L.) callus using disarmed Agrobacterium tumefaciens strainsPlant Cell Reports, 9
K. Ramulu (1986)
CHAPTER 23 – Case Histories of Genetic Variability in Vitro: Potato
A. Karp (1986)
Chromosome Variation in Plants Regenerated From Protoplasts and Cultured Plant Tissues, 20
E. Haute, Henk Joos, M. Maes, G. Warren, M. Montagu, Jozef Schell (1983)
Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens.The EMBO Journal, 2
A. Kathen, H. Jacobsen (1990)
Agrobacterium tumefaciens-mediated transformation of Pisum sativum L. using binary and cointegrate vectorsPlant Cell Reports, 9
J. Puonti-Kaerlas, P. Stabel, T. Eriksson (1989)
Transformation of pea (Pisum sativum L.) byAgrobacterium tumefaciensPlant Cell Reports, 8
M. Holsters, B. Silva, F. Vliet, C. Genetello, M. Block, P. Dhaese, A. Depicker, Dirk Inzé, Gilbert Engler, Raimundo Villarroel, M. Montagu, Jozef Schell (1980)
The functional organization of the nopaline A. tumefaciens plasmid pTiC58.Plasmid, 3 2
O. Gamborg, R. Miller, K. Ojima (1968)
Nutrient requirements of suspension cultures of soybean root cells.Experimental cell research, 50 1
L. Natali, A. Cavallini (1987)
Nuclear cytology of callus and plantlets regenerated from pea (Pisum sativum L.) meristemsProtoplasma, 141
A. Rubluo, K. Kartha, L. Mroginski, J. Dyck (1984)
Plant Regeneration from Pea Leaflets Cultured in vitro and Genetic Stability of Regenerants.Journal of plant physiology, 117 2
Jeffrey Miller (1972)
Experiments in molecular genetics
Patricia Zambryski (1988)
Basic processes underlying Agrobacterium-mediated DNA transfer to plant cells.Annual review of genetics, 22
M. Hinchee, Dannette Connor-Ward, C. Newell, R. Mcdonnell, Shirley Sato, C. Gasser, D. Fischhoff, D. Re, R. Fraley, R. Horsch (1988)
Production of Transgenic Soybean Plants Using Agrobacterium-Mediated DNA TransferBio/Technology, 6
A. Gould, P. King (1984)
Control of the cell cycle in cultured plant cellsCritical Reviews in Plant Sciences, 1
T. Pickardt, M. Meixner, V. Schade, O. Schieder (1991)
Transformation of Vicia narbonensis via Agrobacterium-mediated gene transferPlant Cell Reports, 9
122 84 84 3 4 J. Puonti-Kaerlas T. Eriksson P. Engström Department of Physiological Botany University of Uppsala Box 540 S-751 21 Uppsala Sweden Summary An analysis of the progeny of primary transgenic pea plants in terms of transmission of the transferred DNA, fertility and morphology is presented. A transformation system developed for pea that allows the regeneration of fertile transgenic pea plants from calli selected for antibiotic resistance was used. Expiants from axenic shoot cultures were co-cultivated with a nononcogenic Agrobacterium tumefaciens strain carrying a gene encoding hygromycin phosphotransferase as selectable marker, and transformed callus could be selected on callus-inducing media containing 15 mg/l hygromycin. After several passages on regeneration medium, shoot organogenesis could be reproducibly induced on the hygromycin resistant calli, and the regenerated shoots could subsequently be rooted and transferred to the greenhouse, where they proceeded to flower and set seed. The transmission of the introduced gene into the progeny of the regenerated transgenic plants was studied over two generations, and stable transmission was shown to take place. The transgenic nature of the calli and regenerated plants and their progeny was confirmed by DNA and RNA analysis. The DNA and ploidy levels of the progeny plants and primary regenerants were studied by chromosome analysis, and the offspring of the primary transformants were evaluated morphologically.
TAG Theoretical and Applied Genetics – Springer Journals
Published: Jul 1, 1992
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.