Access the full text.
Sign up today, get DeepDyve free for 14 days.
Peng Liang, A. Pardee (1992)
Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.Science, 257 5072
Peng Liang, Lidia Averboukh, A. Pardee (1993)
Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization.Nucleic acids research, 21 14
F. Li, E. Barnathan, K. Karikó (1994)
Rapid method for screening and cloning cDNAs generated in differential mRNA display: application of northern blot for affinity capturing of cDNAs.Nucleic acids research, 22 9
E. Lasserre, T. Bouquin, José Hernández, J. Pech, C. Balagué, J. Bull (1996)
Structure and expression of three genes encoding ACC oxidase homologs from melon (Cucumis melo L.)Molecular and General Genetics MGG, 251
R. Vögeli-Lange, N. Bürckert, T. Boller, A. Wiemken (1996)
Rapid selection and classification of positive clones generated by mRNA differential display.Nucleic acids research, 24 7
David Bauer, Heiko Muüller, J. Reich, H. Riedel, V. Ahrenkiel, P. Warthoe, Michael Strauss (1993)
Identification of differentially expressed mRNA species by an improved display technique (DDRT-PCR).Nucleic acids research, 21 18
Lunjun Mou, H. Miller, Jing Li, E. Wang, L. Chalifour (1994)
Improvements to the differential display method for gene analysis.Biochemical and biophysical research communications, 199 2
L. Mou, H. Miller, J. Li, E. Wang, L. Chalifour (1994)
Improvements to the differential display method for gene analysis. Biochem. BiophysRes. Comm., 199
A. Hamilton, Grantley Lycett, D. Grierson (1990)
Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plantsNature, 346
(1987)
Current protocols in molecular biology
H. Zhang, R. Zhang, P. Liang (1996)
Differential screening of gene expression difference enriched by differential display.Nucleic acids research, 24 12
The high percentage of false positives generated by differential display (as high as 85%) has previously limited the potential of the method. This report describes an efficient methodology that enables false positives to be discarded prior to cloning, via reverse Northern analysis. This first step of the screening also allows the detection of putative low abundance differential clones. Following cloning, a second reverse Northern combined with partial DNA sequencing and RT-PCR detection allows isolation of all differential cDNAs including very low abundance clones. Use of the sequential screening procedure described here led to the isolation of novel tomato genes responding to the plant hormone ethylene while minimising labor and materials input.
Plant Molecular Biology Reporter – Springer Journals
Published: Sep 29, 2004
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.