Access the full text.
Sign up today, get DeepDyve free for 14 days.
C. Green, J. Needham (1974)
A simple technique for sulphur dressing miceLaboratory Animals, 8
(1972)
H a y a k a w a
M. Greaves, G. Janossy (1972)
Elicitation of Selective T and B Lymphocyte Responses by Cell Surface Binding LigandsImmunological Reviews, 11
J. Cunningham, W. Bruce, H. Webb (1965)
A Convenient 137Cs Unit for Irradiating Cell Suspensions and Small Laboratory AnimalsPhysics in Medicine and Biology, 10
Richard Miller, R. Phillips (1969)
Separation of cells by velocity sedimentationJournal of Cellular Physiology, 73
Darcy Wilson (1967)
QUANTITATIVE STUDIES ON THE MIXED LYMPHOCYTE INTERACTION IN RATSThe Journal of Experimental Medicine, 126
R. Phillips, F. Melchers (1976)
Appearance of functional lymphocytes in fetal liver.Journal of immunology, 117 4
H. Micklem, C. Ford, E. Evans, Joyce Gray (1966)
Interrelationships of myeloid and lymphoid cells: studies with chromosome-marked cells transfused into lethally irradiated miceProceedings of the Royal Society of London. Series B. Biological Sciences, 165
W. Bruce, H. Gaag (1963)
A Quantitative Assay for the Number of Murine Lymphoma Cells capable of Proliferation in vivoNature, 199
(1972)
Phytohemagglutinin and concanavalin A: probes for murine T cell activation and differentiation
J. Parker, D. Metcalf (1974)
Production of colony-stimulating factor in mixed leucocyte cultures.Immunology, 26 5
J. Till, E. McCulloch (1961)
A direct measurement of the radiation sensitivity of normal mouse bone marrow cells.Radiation research, 14
J. Batchelor (1965)
Conceptual Advances in Immunology and Oncology
Richard Miller, R. Gorczynski, L. Lafleur, H. Macdonald, R. Phillips (1975)
Cell Separation Analysis of B and T Lymphocyte DifferentiationImmunological Reviews, 25
(1971)
Two types of ribosomes in mousehamster hybrid cells
P. Nowell, B. Hirsch, D. Fox, Darcy Wilson (1970)
Evidence for the existence of multipotential lympho‐hematopoietic stem cells in the adult ratJournal of Cellular Physiology, 75
D. Barnes, E. Evans, C. Ford, B. West (1968)
Spleen Colonies in Mice: Karyotypic Evidence of Multiple Colonies from Single CellsNature, 219
G. Edwards, R. Miller, R. Phillips (1970)
Differentiation of rosette-forming cells from myeloid stem cells.Journal of immunology, 105 3
H. Vogel, H. Niewisch, G. Matioli (1968)
The self renewal probability of hemopoietic stem cellsJournal of Cellular Physiology, 72
(1972)
Radiation-induced leukemia in C57BL/6J mice
J. Till, E. McCulloch, L. Siminovitch (1964)
A STOCHASTIC MODEL OF STEM CELL PROLIFERATION, BASED ON THE GROWTH OF SPLEEN COLONY-FORMING CELLS.Proceedings of the National Academy of Sciences of the United States of America, 51
E. McCulloch, L. Siminovitch, J. Till (1964)
Spleen-Colony Formation in Anemic Mice of Genotype WWScience, 144
M. Cooper, R. Keightley, Liang‐Yeh Wu, A. Lawton (1973)
Developmental Defects of T and B Cell Lines in HumansImmunological Reviews, 16
C. Ford, J. Hamerton, D. Barnes, J. Loutit (1956)
Cytological Identification of Radiation-ChimærasNature, 177
Kornilova Ap, Henkelman Rm, Ottensmeyer Fp, J. Till (1973)
Investigations of a stochastic model of haemopoiesis.Experimental hematology, 1 6
A. Wu, J. Till, L. Siminovitch, E. McCulloch (1968)
CYTOLOGICAL EVIDENCE FOR A RELATIONSHIP BETWEEN NORMAL HEMATOPOIETIC COLONY-FORMING CELLS AND CELLS OF THE LYMPHOID SYSTEMThe Journal of Experimental Medicine, 127
J. Trentin, N. Wolf, V. Cheng, W. Fahlberg, D. Weiss, R. Bonhag (1967)
Antibody production by mice repopulated with limited numbers of clones of lymphoid cell precursors.Journal of immunology, 98 6
J. Kadish, R. Basch (1976)
Hematopoietic thymocyte precursors. I. Assay and kinetics of the appearance of progenyThe Journal of Experimental Medicine, 143
Ken Shortman, Neil Williams, Peter Adams (1972)
The separation of different cell classes from lymphoid organs. V. Simple procedures for the removal of cell debris. Damaged cells and erythroid cells from lymphoid cell suspensions.Journal of immunological methods, 1 3
J. Andersson, O. Sjöberg, G. Möller (1972)
Mitogens as Probes for Immunocyte Activation and Cellular CooperationImmunological Reviews, 11
A. Wu, A. Wu, J. Till, J. Till, L. Siminovitch, L. Siminovitch, E. McCulloch, E. McCulloch (1967)
A cytological study of the capacity for differentiation of normal hemopoietic colony‐forming cellsJournal of Cellular Physiology, 69
J. Trentin, W. Fahlberg (1962)
An Experimental Model for Studies of Immunological Competence in Irradiated Mice Repopulated with “Clones” of Spleen CellsPlastic and Reconstructive Surgery, 30
(1969)
Physical separation of hemopoietic stem cells forming colonies in culture
(1967)
Radiation Chimaeras. Logos
L. Yung, T. Wyn-Evans, E. Diener (1973)
Ontogeny of the murine immune system: development of antigen recognition and immune responsivenessEuropean Journal of Immunology, 3
H. Micklem, J. Loutit (1967)
Tissue grafting and radiationThe American Journal of the Medical Sciences, 253
The precise relationship between the stem cells for the lymphoid system and those for the blood-forming system is unclear. While it is generally assumed that the hemopoietic stem cell, the spleen colony-forming unit (CFU-S), is also the stem cell for the lymphoid system, there is little evidence for this hypothesis. To investigate the stem cells in these two systems, we irradiated bone marrow cells to induce unique chromosome aberrations in the stem cell population and injected them at limiting dilution into stem cell-deficient recipients. Several months (between 3 and 11) were allowed for the injected cells to repopulate the hemopoietic system. At that time, the bone marrow, spleen, and thymus were examined for a high frequency of cells having the same unique chromosome aberration. The presence of such markers shows that the marker was induced in a cell with extensive proliferative capacity, i.e., a stem cell. In addition, the splenic lymphocytes were stimulated with phytohemagglutinin (PHA) or lipopolysaccharide (LPS) to search for unique chromosomes in dividing T and B cells, respectively. Finally, bone marrow cells were injected into secondary irradiated recipients to determine if the marker occurred in CFU-S and to determine whether or not the same tissue distributions of marked cells could be propogated by bone marrow cells in a second recipient. After examination of 28 primary recipients, it was possible to identify three unique patterns of stem cell regeneration. In one set of mice, a unique chromosome marker was observed in CFU-S and in PHA- and LPS-stimulated cultures. These mice provide direct evidence for a pluripotent stem cell in bone marrow. In addition, two restricted stem cells were identified by this analysis. In three recipients, abnormal karyotypes were found only in myeloid cells and not in B and T lymphocytes. These mice presumably received a marked stem cell restricted to differentiate only into myeloid progeny. In three other recipients, chromosome aberrations were found only in PHA-stimulated cells; CFU-S and cells from LPS cultures did not have cells with the unique chromosome. This pattern suggests that bone marrow contains cells committed to differentiation only into T lymphocytes. For each of the three types of stem cells, secondary recipients had the same cellular distribution of marked cells as the primary recipients. This observation provides further evidence that unique markers can be induced in both pluripotent and restricted stem cells.
The Journal of Experimental Medicine – Rockefeller University Press
Published: Jun 1, 1977
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.