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References (41)
-
Jianru Zuo, Qinglin Niu, N. Chua (2000)
Technical advance: An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants.The Plant journal : for cell and molecular biology, 24 2
-
H. Chung, R. Ferl (1999)
Arabidopsis alcohol dehydrogenase expression in both shoots and roots is conditioned by root growth environment.Plant physiology, 121 2
-
Alberto Martinez, Caroline Sparks, Cliff Hart, John Thompson, Ian Jepson (1999)
Ecdysone agonist inducible transcription in transgenic tobacco plants.The Plant journal : for cell and molecular biology, 19 1
-
S. Böhner, I. Lenk, M. Rieping, Michael Herold, C. Gatz (1999)
Technical advance: transcriptional activator TGV mediates dexamethasone-inducible and tetracycline-inactivatable gene expressionThe Plant journal : for cell and molecular biology, 19 1
-
Farfan (1999)
25Promega Notes, 70
-
M. Sachs, M. Freeling, R. Okimoto (1980)
The anaerobic proteins of maizeCell, 20
-
D. Becker, E. Kemper, J. Schell, R. Masterson (1992)
New plant binary vectors with selectable markers located proximal to the left T‐DNA borderPlant Mol. Biol., 20
-
Bronstein (1994)
172Biotechniques, 17
-
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual -
S. Bohner, I. Kebjm, M. Rieping, M. Herold, C. Gatz (1999)
Transcriptional activator TGV mediates dexamethasone‐inducible and tetracycline‐inactivatable gene expressionPlant J., 19
-
H. Azman. (1999)
A chemically inducible gene expression system for use in Arabidopsis thaliana. -
L. Luzzatto, M. Bessler, B. Rotoli (1997)
Somatic Mutations in Paroxysmal Nocturnal Hemoglobinuria: A Blessing in Disguise?Cell, 88
-
R. Jefferson, T. Kavanagh, M. Bevan (1987)
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.The EMBO Journal, 6
-
(2000)
Bontrol of the coinducer of ethanol catabolism in Aspergillus nidulan s -
W. Bruce, O. Folkerts, C. Garnaat, O. Crasta, Brad Roth, B. Bowen (2000)
Expression Profiling of the Maize Flavonoid Pathway Genes Controlled by Estradiol-Inducible Transcription Factors CRC and PPlant Cell, 12
-
S. Clough, A. Bent (1998)
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.The Plant journal : for cell and molecular biology, 16 6
-
Michael White, Christopher Wood, Andrew Millar (1996)
Real-time imaging of transcription in living cells and tissues.Biochemical Society transactions, 24 3
-
P. Weinmann, M. Gossen, W. Hillen, H. Bujard, C. Gatz (1994)
A chimeric transactivator allows tetracycline-responsive gene expression in whole plants.The Plant journal : for cell and molecular biology, 5 4
-
M. Metzlaff, Michael O'dell, P. Cluster, Richard Flavell (1997)
RNA-Mediated RNA Degradation and Chalcone Synthase A Silencing in PetuniaCell, 88
-
(1999)
Eukaryotic expression: transfection and reporter vectors -
De Wilde (2000)
347Plant Mol Biol., 43
-
Mark Caddick, A. Greenland, lan Jepson, K. Krause, N. Qu, K. Riddell, M. Salter, W. Schuch, U. Sonnewald, A. Tomsett (1998)
An ethanol inducible gene switch for plants used to manipulate carbon metabolismNature Biotechnology, 16
-
J. Odell, F. Nagy, N. Chua (1985)
Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoterNature, 313
-
D. Gwynne, F. Buxton, S. Sibley, R. Davies, R. Lockington, C. Scazzocchio, H. Sealy-Lewis (1987)
Comparison of the cis-acting control regions of two coordinately controlled genes involved in ethanol utilization in Aspergillus nidulans.Gene, 51 2-3
-
M. Salter, Jacqueline Paine, K. Riddell, I. Jepson, A. Greenland, M. Caddick, A. Tomsett (1998)
Characterisation of the ethanol‐inducible alc gene expression system for transgenic plantsPlant Journal, 16
-
C. Gatz, Claus Frohberg, Regina Wendenburg (1992)
Stringent repression and homogeneous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants.The Plant journal : for cell and molecular biology, 2 3
-
Bechtold (1993)
1194Comptes Rend. Acad. Sci. Ser. III, 316
-
N. Bechtold, J. Ellis, G. Pelletier (1993)
In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants, 316
-
T. Aoyama, N. Chua (1997)
A glucocorticoid-mediated transcriptional induction system in transgenic plants.The Plant journal : for cell and molecular biology, 11 3
-
C. De Wilde, H. Van Houdt, S. De Buck, G. Angenon, G. De Jaeger, A. Depicker (2000)
Plants as bioreactors for protein production: avoiding the problem of transgene silencingPlant Mol Biol., 43
-
Becker (1992)
1195Plant Mol. Biol., 20
-
M. Bradford (1976)
A rapid and sensitive method for quantification of microgram quantities of protein using the principle of protein dye bindingAnn. Biochem., 72
-
Johnf Thompson, L. Hayes, David Lloyd (1991)
Modulation of firefly luciferase stability and impact on studies of gene regulation.Gene, 103 2
-
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
-
A. Millar, S. Short, N. Chua, S. Kay (1992)
A novel circadian phenotype based on firefly luciferase expression in transgenic plants.The Plant cell, 4
-
T. Hayakawa, K. Kinoshita, S. Miyaki, H. Fujiwake, S. Ohsuka (1986)
ULTRA‐LOW‐LIGHT LEVEL CAMERA FOR PHOTON COUNTING IMAGINGPhotochemistry and Photobiology, 43
-
R. Dolferus, M. Jacobs, W. Peacock, E. Dennis (1994)
Differential Interactions of Promoter Elements in Stress Responses of the Arabidopsis Adh Gene, 105
-
R. Lockington, H. Sealy-Lewis, C. Scazzocchio, R. Davies (1985)
Cloning and characterization of the ethanol utilization regulon in Aspergillus nidulans.Gene, 33 2
-
I. Bronstein, J. Fortin, J. Voyta, R. Juo, B. Edwards, C. Olesen, N. Lijam, L. Kricka (1994)
Chemiluminescent reporter gene assays: sensitive detection of the GUS and SEAP gene products.BioTechniques, 17 1
-
M. Bevan, W. Barnes, Marry-Dell Chilton (1983)
Structure and transcription of the nopaline synthase gene region of T-DNA.Nucleic acids research, 11 2
-
M. Pickett, D. Gwynne, F. Buxton, R. Elliott, R. Davies, R. Lockington, C. Scazzocchio, H. Sealy-Lewis (1987)
Cloning and characterization of the aldA gene of Aspergillus nidulans.Gene, 51 2-3
- Publisher
- Wiley
- Copyright
- Copyright © 2001 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 0960-7412
- eISSN
- 1365-313X
- DOI
- 10.1046/j.1365-313X.2001.01146.x
- Publisher site
- See Article on Publisher Site
Abstract
Controlled expression of transgenes in plants is key to the characterization of gene function and the regulated manipulation of growth and development. The alc gene‐expression system, derived from the filamentous fungus Aspergillus nidulans, has previously been used successfully in both tobacco and potato, and has potential for use in agriculture. Its value to fundamental research is largely dependent on its utility in Arabidopsis thaliana. We have undertaken a detailed function analysis of the alc regulon in A. thaliana. By linking the alcA promoter to β‐glucuronidase (GUS), luciferase (LUC) and green fluorescent protein (GFP) genes, we demonstrate that alcR‐mediated expression occurs throughout the plant in a highly responsive manner. Induction occurs within one hour and is dose‐dependent, with negligible activity in the absence of the exogenous inducer for soil‐grown plants. Direct application of ethanol or exposure of whole plants to ethanol vapour are equally effective means of induction. Maximal expression using soil‐grown plants occurred after 5 days of induction. In the majority of transgenics, expression is tightly regulated and reversible. We describe optimal strategies for utilizing the alc system in A. thaliana.
Journal
The Plant Journal – Wiley
Published: Oct 1, 2001
Keywords: ; ; ; ;