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Bacillus subtilis spores: A novel microparticle adjuvant which can instruct a balanced Th1 and Th2 immune response to specific antigen

Bacillus subtilis spores: A novel microparticle adjuvant which can instruct a balanced Th1 and... There is a current need for safe, cheap, and effective vaccine adjuvants, to combine with sub‐unit antigens to enhance their immunogenicity. In this study we have used probiotic Bacillus subtilis spores, known to be safe and fully tolerated by ingestion in man, and explored their ability to influence the magnitude and diversity of immune responses induced against two model antigens, tetanus toxoid fragment C (TT) and ovalbumin (OVA) in mice. The results show that B. subtilis spores not only increased antibody and T cell responses to a co‐administered soluble antigen, but also broadened them, to include both antigen‐specific CD4+ and CD8+ T cell responses as well as complement and non‐complement fixing antibody isotypes. Furthermore, following intranasal immunization, spores augmented specific IgA to co‐administered antigen both in the local respiratory and distal vaginal mucosa, as well as increased antigen‐specific IgG antibody in draining LN and blood. Collectively, these data demonstrate that naturally occurring, non‐pathogenic, non‐commensal spores of B. subtilis both instruct and augment polyvalent immune responses and highlight their clinical potential in future vaccines to generate broad‐based immunity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Immunology Wiley

Bacillus subtilis spores: A novel microparticle adjuvant which can instruct a balanced Th1 and Th2 immune response to specific antigen

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References (52)

Publisher
Wiley
Copyright
Copyright © 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
ISSN
0014-2980
eISSN
1521-4141
DOI
10.1002/eji.200636875
pmid
17474150
Publisher site
See Article on Publisher Site

Abstract

There is a current need for safe, cheap, and effective vaccine adjuvants, to combine with sub‐unit antigens to enhance their immunogenicity. In this study we have used probiotic Bacillus subtilis spores, known to be safe and fully tolerated by ingestion in man, and explored their ability to influence the magnitude and diversity of immune responses induced against two model antigens, tetanus toxoid fragment C (TT) and ovalbumin (OVA) in mice. The results show that B. subtilis spores not only increased antibody and T cell responses to a co‐administered soluble antigen, but also broadened them, to include both antigen‐specific CD4+ and CD8+ T cell responses as well as complement and non‐complement fixing antibody isotypes. Furthermore, following intranasal immunization, spores augmented specific IgA to co‐administered antigen both in the local respiratory and distal vaginal mucosa, as well as increased antigen‐specific IgG antibody in draining LN and blood. Collectively, these data demonstrate that naturally occurring, non‐pathogenic, non‐commensal spores of B. subtilis both instruct and augment polyvalent immune responses and highlight their clinical potential in future vaccines to generate broad‐based immunity.

Journal

European Journal of Immunology – Wiley

Published: Jun 1, 2007

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